人翻译起始因子eIF4B c端区域的主链共振分配。

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2023-06-27 DOI:10.1007/s12104-023-10141-7

Somnath Mondal, Sabrina Rousseau, Vincent Talenton, Cheikh Ahmadou Bamba Thiam, Mikayel Aznauryan, Cameron D. Mackereth

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引用次数: 0

摘要

真核生物中的翻译起始是蛋白质合成的早期步骤，需要多种因素将核糖体小亚基募集到信使核糖核酸5'非翻译区。一种这样的蛋白质因子是真核翻译起始因子4B（eIF4B），其增加eIF4A RNA解旋酶的活性，并与细胞存活和增殖有关。我们在此报道了与人eIF4B的C末端279个残基相对应的蛋白质骨架化学位移分配。化学位移值的分析确定了先前与RNA结合相连的区域中的一个主要螺旋区域，并证实了整个C末端区域本质上是无序的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Backbone resonance assignments of the C-terminal region of human translation initiation factor eIF4B

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Backbone resonance assignments of the C-terminal region of human translation initiation factor eIF4B

Translation initiation in eukaryotes is an early step in protein synthesis, requiring multiple factors to recruit the ribosomal small subunit to the mRNA 5’ untranslated region. One such protein factor is the eukaryotic translation initiation factor 4B (eIF4B), which increases the activity of the eIF4A RNA helicase, and is linked to cell survival and proliferation. We report here the protein backbone chemical shift assignments corresponding to the C-terminal 279 residues of human eIF4B. Analysis of the chemical shift values identifies one main helical region in the area previously linked to RNA binding, and confirms that the overall C-terminal region is intrinsically disordered.

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.