海藻积累聚(3-羟基丁酸酯)的Cobetia sp. IU180733JP01(5-11-6-3)转化偶联方法的建立。

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yuki Umebayashi, Shiori Abe, Miwa Yamada
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引用次数: 0

摘要

由于其生长速度快,细胞密度高,嗜盐性好,因此被认为是工业应用的有用宿主。本研究构建了一种高效的偶联方法,获得了Cobetia sp. IU180733JP01(5-11-6-3)转化体,该转化体可以利用海藻酸盐或海藻废弃物生产生物塑料。采用含2% NaCl的溶原肉汤培养基与大肠埃希菌S17-1(质粒供体)共培养5-11-6-3菌株(质粒受体)。在供体:受体细胞数比为5:1时,获得了最高共轭效率[(2.92±1.37)× 10-3]的转化子。这是第一个报道在Cobetia属中创建重组菌株的研究。这种方法将有助于创造一个平台菌株,用于从海洋生物质中生产生物塑料和其他有用的材料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Construction of a conjugation method for the transformation of Cobetia sp. IU180733JP01 (5-11-6-3) that accumulates poly(3-hydroxybutyrate) from seaweeds.

Cobetia bacteria are considered useful hosts for industrial applications owing to their fast growth, high cell density, and halophilicity. Here, we constructed an efficient conjugation method to obtain Cobetia sp. IU180733JP01 (5-11-6-3) transformants, which can produce bioplastics from alginate or seaweed waste. Lysogeny Broth medium containing 2% NaCl was used to co-cultivate the 5-11-6-3 strain (plasmid recipient) with Escherichia coli S17-1 (plasmid donor). Transformants with the highest conjugation efficiency [(2.92 ± 1.37) × 10-3] were obtained at a donor:recipient cell number ratio of 5:1. This is the first study reporting the creation of recombinant strains in the genus Cobetia. This method will contribute to creating a platform strain for the production of bioplastics and other useful materials from marine biomass.

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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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