同时眼内和眼内注射伏立康唑对兔角膜内皮的毒性作用。

IF 1.6 4区 医学 Q3 OPHTHALMOLOGY
Ji Young Moon, Wool Suh, Roo Min Jun, Kyung Eun Han
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引用次数: 0

摘要

目的:观察伏立康唑反复同时注射对兔角膜内皮细胞的毒性。方法:36只新西兰大白兔18只,每组6只,按伏立康唑浓度分为6组(A组,0%;B组,0.05%;C组,0.1%;D组,0.25%;E组,0.5%;F组,1%)。各组分别于第0、3、7天眼内联合注射伏立康唑3次。分别于第0、3、7、10、14天检测角膜混浊等级和角膜中央厚度(CCT)。分别于第0、14天测定内皮细胞计数(ECC)。第14天进行扫描电镜(SEM)和透射电镜(TEM)检查。结果:从第7天开始,F组(1%)的角膜混浊程度明显高于其他各组(A-E组)(p < 0.05)。扫描电镜显示浓度≥0.25%时细胞边界模糊,微绒毛缺失(D-F组)。电镜显示,浓度≥0.1% (C-F组)时内皮细胞微结构发生改变,1%伏立康唑(F组)时内皮细胞出现多个空泡。结论:0.1%及以上浓度的伏立康唑反复同时注射可诱导兔角膜内皮细胞微结构内皮损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Toxicity of simultaneous intrastromal and intracameral injection of voriconazole on corneal endothelium in a rabbit model.

Purpose: To investigate the toxicity of repeated simultaneous intrastromal and intracameral injections of voriconazole in corneal endothelial cells in a rabbit model.

Methods: Thirty-six eyes of 18 New Zealand white rabbits (six eyes per group) were divided into 6 groups according to the concentration of voriconazole (Group A, 0%; Group B, 0.05%; Group C, 0.1%; Group D, 0.25%; Group E, 0.5%; Group F, 1%). A combination of intrastromal and intracameral voriconazole injections were administrated to the eyes of each group three times on days 0, 3, and 7. Corneal clouding grades and central corneal thickness (CCT) were examined on days 0, 3, 7, 10, and 14. The endothelial cell counts (ECC) were measured on days 0 and 14. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were performed on day 14.

Results: Group F (1%) showed more severe corneal clouding than the other groups (Groups A-E) from day 7 (p < 0.05, respectively). There were no significant differences in CCT and ECC among the six groups at any time point (p > 0.05, respectively). SEM revealed blurring of the cell border and loss of microvilli at concentrations ≥0.25% (Groups D-F). TEM revealed microstructural changes in endothelial cells at concentrations ≥0.1% (Groups C-F), and multiple vacuoles were observed at a concentration of 1% voriconazole (Group F).

Conclusions: Repeated simultaneous intrastromal and intracameral voriconazole injections at a concentration of 0.1% or higher induced microstructural endothelial damage in rabbit corneal endothelial cells.

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来源期刊
CiteScore
3.30
自引率
6.20%
发文量
40
审稿时长
1 months
期刊介绍: Cutaneous and Ocular Toxicology is an international, peer-reviewed journal that covers all types of harm to cutaneous and ocular systems. Areas of particular interest include pharmaceutical and medical products; consumer, personal care, and household products; and issues in environmental and occupational exposures. In addition to original research papers, reviews and short communications are invited, as well as concise, relevant, and critical reviews of topics of contemporary significance.
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