植入前牛胚胎中DKK1的KREMEN受体的存在。

Thiago Fernandes Amaral, Yao Xiao, Surawich Jeensuk, Tatiane Silva Maia, Camila J Cuellar, Chloe A Gingerich, Tracy L Scheffler, Peter J Hansen
{"title":"植入前牛胚胎中DKK1的KREMEN受体的存在。","authors":"Thiago Fernandes Amaral,&nbsp;Yao Xiao,&nbsp;Surawich Jeensuk,&nbsp;Tatiane Silva Maia,&nbsp;Camila J Cuellar,&nbsp;Chloe A Gingerich,&nbsp;Tracy L Scheffler,&nbsp;Peter J Hansen","doi":"10.1530/RAF-23-0021","DOIUrl":null,"url":null,"abstract":"<p><p>The WNT inhibitory protein DKK1 has been shown to regulate development of the preimplantation embryo to the blastocyst stage. In cattle, DKK1 increases the number of trophectoderm cells that are the precursor of the placenta. DKK1 can affect cells by blocking WNT signaling through its receptors KREMEN1 and KREMEN2. Here it was shown that the mRNA for KREMEN1 and KREMEN2 decline as the embryo advances in development. Nonetheless, immunoreactive KREMEN1 was identified in blastocysts using Western blotting. DKK1 also decreased amount of immunoreactive CTNNB1 in blastocysts, as would be expected if DKK1 was signaling through a KREMEN-mediated pathway. Thus, it is likely that KREMEN1 functions as a receptor for DKK1 in the preimplantation bovine embryo.</p>","PeriodicalId":21128,"journal":{"name":"Reproduction & Fertility","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10620448/pdf/","citationCount":"0","resultStr":"{\"title\":\"Presence of KREMEN receptors for DKK1 in the preimplantation bovine embryo.\",\"authors\":\"Thiago Fernandes Amaral,&nbsp;Yao Xiao,&nbsp;Surawich Jeensuk,&nbsp;Tatiane Silva Maia,&nbsp;Camila J Cuellar,&nbsp;Chloe A Gingerich,&nbsp;Tracy L Scheffler,&nbsp;Peter J Hansen\",\"doi\":\"10.1530/RAF-23-0021\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The WNT inhibitory protein DKK1 has been shown to regulate development of the preimplantation embryo to the blastocyst stage. In cattle, DKK1 increases the number of trophectoderm cells that are the precursor of the placenta. DKK1 can affect cells by blocking WNT signaling through its receptors KREMEN1 and KREMEN2. Here it was shown that the mRNA for KREMEN1 and KREMEN2 decline as the embryo advances in development. Nonetheless, immunoreactive KREMEN1 was identified in blastocysts using Western blotting. DKK1 also decreased amount of immunoreactive CTNNB1 in blastocysts, as would be expected if DKK1 was signaling through a KREMEN-mediated pathway. Thus, it is likely that KREMEN1 functions as a receptor for DKK1 in the preimplantation bovine embryo.</p>\",\"PeriodicalId\":21128,\"journal\":{\"name\":\"Reproduction & Fertility\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10620448/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reproduction & Fertility\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1530/RAF-23-0021\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproduction & Fertility","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1530/RAF-23-0021","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

WNT抑制蛋白DKK1已被证明可调节植入前胚胎发育至胚泡阶段。在牛中,DKK1增加了作为胎盘前体的滋养外胚层细胞的数量。DKK1可以通过其受体KREMEN1和KREMEN2阻断WNT信号传导来影响细胞。研究表明,KREMEN1和KREMEN2的mRNA随着胚胎发育的进行而下降。尽管如此,免疫反应性KREMEN1在胚泡中通过蛋白质印迹鉴定。DKK1还降低了胚泡中免疫反应性CTNNB1的量,这是可以预期的,如果DKK1通过KREMEN介导的途径发出信号。因此,KREMEN1可能在植入前的牛胚胎中作为DKK1的受体发挥作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Presence of KREMEN receptors for DKK1 in the preimplantation bovine embryo.

Presence of KREMEN receptors for DKK1 in the preimplantation bovine embryo.

Presence of KREMEN receptors for DKK1 in the preimplantation bovine embryo.

Presence of KREMEN receptors for DKK1 in the preimplantation bovine embryo.

The WNT inhibitory protein DKK1 has been shown to regulate development of the preimplantation embryo to the blastocyst stage. In cattle, DKK1 increases the number of trophectoderm cells that are the precursor of the placenta. DKK1 can affect cells by blocking WNT signaling through its receptors KREMEN1 and KREMEN2. Here it was shown that the mRNA for KREMEN1 and KREMEN2 decline as the embryo advances in development. Nonetheless, immunoreactive KREMEN1 was identified in blastocysts using Western blotting. DKK1 also decreased amount of immunoreactive CTNNB1 in blastocysts, as would be expected if DKK1 was signaling through a KREMEN-mediated pathway. Thus, it is likely that KREMEN1 functions as a receptor for DKK1 in the preimplantation bovine embryo.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信