Central European Journal of Biology最新文献_第6页

The Effects of SARS CoV-2 nsp13 Mutations on the Structure and Stability of Helicase in Chinese Isolates SARS CoV-2 nsp13突变对中国分离株解旋酶结构和稳定性的影响

Central European Journal of Biology Pub Date : 2022-04-13 DOI: 10.26650/eurjbiol.2022.1061858

Ekrem Akbulut

{"title":"The Effects of SARS CoV-2 nsp13 Mutations on the Structure and Stability of Helicase in Chinese Isolates","authors":"Ekrem Akbulut","doi":"10.26650/eurjbiol.2022.1061858","DOIUrl":"https://doi.org/10.26650/eurjbiol.2022.1061858","url":null,"abstract":"Objective: Coronavirus Disease 2019 (COVID19) is a viral disease caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2). The high mutation propensity of the SARS CoV-2 genome is one of the biggest threats to the long-term validity of treatment options. Helicases are anti-viral targets because of the vital role they play in the viral life cycle. In this study, changes in the protein structure caused by SARS CoV-2 nsp13 mutations were investigated to contribute to the development of effective antiviral drugs. Materials and Methods: Genome data of 298 individuals located in the China location were examined. The mutant model was built using deep learning algorithms. Model quality assessment was done with QMEAN. Protein stability analyses were performed with DynaMut2 and Cutoff Scanning Matrix stability. Changes in substrate affinity were performed with Haddock v2.4. Results: In this study, twenty-eight mutations in nsp13 were identified (23 sense, 5 missense). The changes in protein structure caused by the five missense mutations (Leu14Phe, Arg15Ser, Arg21Ser, Leu235Phe, Ala454Thr) were modeled. The mutations caused a decrease in the stability of SARS CoV-2 helicase (-0.99, -1.66, -1.15, -0.54, and -0.73 for Leu14Phe, Arg15Ser, Arg21Ser, Leu235Phe, Ala454Thr, respectively). The mutations reduced the helicase's affinity to the substrate. The docking scores for wild-type and mutant helicase were -84.4±1.4 kcal.mol-1 and -71.1±6.7 kcal.mol-1, respectively. Conclusion: Helicase mutations caused a decrease in the protein stability and nucleic acid affinity of the SARS CoV-2 helicase. The results provide important data on the development of potential antivirals and the effect of mutation on the functions of viral proteins. © European Journal of Biology.All rights reserved.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81461900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Profiling and Wetting Behaviors of Endemic Salvia absconditiflora Greuter & Burdet (Lamiaceae) Collected from Gypsum Areas 石膏区特有绝育鼠尾草(Lamiaceae)的化学特征和润湿行为

Central European Journal of Biology Pub Date : 2022-01-27 DOI: 10.26650/eurjbiol.2021.1011530

Ayşenur Kayabaş, E. Yıldırım

{"title":"Chemical Profiling and Wetting Behaviors of Endemic Salvia absconditiflora Greuter & Burdet (Lamiaceae) Collected from Gypsum Areas","authors":"Ayşenur Kayabaş, E. Yıldırım","doi":"10.26650/eurjbiol.2021.1011530","DOIUrl":"https://doi.org/10.26650/eurjbiol.2021.1011530","url":null,"abstract":"Objective: Salvia absconditiflora Greuter & Burdet is an endemic plant and survives in nature by adapting to extreme conditions. The aims of this study are to characterize and compare the diversity in the spectral-chemical structure of S. absconditiflora’s plant parts using the FTIR spectroscopy technique, to determine the wettability of the adaxial and abaxial epidermal surfaces of S. abscontidiflora leaves and to interpret whether there is a difference between the contact angle (CA) measurements at the points determined in the surface area of the leaves from the part close to the petiole to the leaf tip. Materials and Methods: The ATR-FTIR spectra for the chemical content of S. absconditiflora were obtained from six different plant parts and information about their chemical compositions was obtained. CA measurements were carried out for the natural events of the leaf area, especially for the water holding capacity or hydrophilic-hydrophobic characteristics. Results: The biochemical fingerprint of S. absconditiflora was determined by the analysis of chemical groups in vegetative and generative plant parts using ATR-FTIR spectroscopy. The CAs showed that the leaf had a hydrophobic character. In addition, leaf hysteresis was determined for each plant part, and it was understood that the lotus effect also appeared in S. absconditiflora. Conclusion: Detailed biochemical profiling, wettability, and hysteresis reports of S. absconditiflora were created for the first time. With this study, important clues about the adaptation of plants to harsh conditions were obtained.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77176276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human T-cell lymphotropic virus type 1 oncogenesis and cell-to-cell spread 人类t细胞嗜淋巴病毒1型肿瘤发生和细胞间扩散

Central European Journal of Biology Pub Date : 2022-01-20 DOI: 10.47672/ejb.913

Tehreem Khalil, Samman Samman, Z. Jawad, Zunaira Hakeem, Aemin Rasheed, Fareeha Sohail, Iqra Asad, Shehreen Sohail, Hamza Rana, S. Saleem

{"title":"Human T-cell lymphotropic virus type 1 oncogenesis and cell-to-cell spread","authors":"Tehreem Khalil, Samman Samman, Z. Jawad, Zunaira Hakeem, Aemin Rasheed, Fareeha Sohail, Iqra Asad, Shehreen Sohail, Hamza Rana, S. Saleem","doi":"10.47672/ejb.913","DOIUrl":"https://doi.org/10.47672/ejb.913","url":null,"abstract":"Exploring host-HTLV-1 interactions and the molecular processes underpinning HTLV-1-mediated carcinogenesis is crucial for establishing effective treatments for viral infection and associated leukemia/lymphoma. Several HTLV-1 proteins have been shown to play important roles in the cellular transformation and immortalization of infected T cells. Through interactions with MAVS, STING, and RIP1, the HTLV-1 oncoprotein Tax suppresses the innate IFN response, resulting in the inhibition of TBK1-mediated phosphorylation of IRF3/IRF7. The HTLV-1 protein HBZ affects genomic integrity and inhibits target cell death and autophagy. Furthermore, it has been discovered that HBZ promotes the growth of ATL cells and aids in the evasion of infected cells from immunosurveillance. It currently appears that the efficacy of an individual's cytotoxic T cell (CTL) response to HTLV-1 is the most important single predictor of that person's provirus load, which can differ by more than 10,000-fold amongst HTLV-1-infected persons. We examine recent improvements in our knowledge of the pathophysiology, or underlying processes, of the illness produced by HTLV-1 infection in this article. Furthermore, we explore the future approach for targeting HTLV-1-associated malignancies and anti-HTLV-1 therapies. The pathogenic agent of adult T-cell leukemia/lymphoma (ATL) is human T-cell lymphotropic virus type 1 (HTLV-1). ATL is a fast-developing clonal tumour of CD4+ T cells which are cellular and viral protein interactions and pave the way for the discovery of new classes of cellular modulators, which may induce Tax oncogenesis and its impact on survival signalling pathways such as the NF-B and PI3K-Akt pathways, therapeutic target opportunities for ATL have been presented in two collaborative studies.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84338774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effects of Petroselinum crispum (Parsley) Extract Against Methotrexate-Induced Hepatotoxicity 欧芹提取物对甲氨蝶呤所致肝毒性的保护作用

Central European Journal of Biology Pub Date : 2021-12-15 DOI: 10.26650/eurjbiol.2021.1023136

B. Ertaş, F. Turan, Dilek Özbeyli, R. Yanardağ, Özlem Saçan, G. Şener

引用次数: 3

Scape, Rhizome and Root Anatomy of Polygonatum Species from Turkey 土耳其黄精属植物的茎、茎和根解剖

Central European Journal of Biology Pub Date : 2021-12-15 DOI: 10.26650/eurjbiol.2021.1026981

Yeter Yeşil Cantürk, F. Özhatay

{"title":"Scape, Rhizome and Root Anatomy of Polygonatum Species from Turkey","authors":"Yeter Yeşil Cantürk, F. Özhatay","doi":"10.26650/eurjbiol.2021.1026981","DOIUrl":"https://doi.org/10.26650/eurjbiol.2021.1026981","url":null,"abstract":"Objective: Polygonatum (Asparagaceae) is a rhizomatous monocotyledon genus that is distributed in the temperate regions of the Northern Hemisphere. The genus is represented by 5 species in Turkey (P. glaberrimum, P. latifolium, P. multiflorum, P. verticillatum and P. orientale). Karyological, palynological, phylogenic, chemotaxonomical and some anatomical studies of Polygonatum species have been carried out previously. However, the detailed anatomical features of the scape, rhizome and root of Polygonatum species grown in Turkey have not been examined before and so the aim of this study is to determine their anatomical characteristics. Also, the study compares the species with each other according to obtained anatomical characteristics and comparing them with previous anatomical studies. Materials and Methods: Investigations were carried out on fresh material collected from wild habitats during July 20092012 in Turkey. Cross-sections of scape, rhizome and roots were cut by hand, detailed photographs were taken under a light microscope, and anatomical structures were drawn in detail with an isograph pen. Results: The anatomical structures of Polygonatum species have monocotyledones features. Significant differences between species were observed, such as the number of costas in the scape, number of xylem arms in the root and the types of vascular bundle in the rhizome. Also, the anatomical characteristics of the species were compared with previous anatomical studies. Conclusion: The anatomical features determined by this study will be useful at the species level as well as at the genus and family level. Hence the obtained data will contribute to the literature.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90670577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant Activities of Eremurus spectabilis M. Bieb. Extracts and Sulfur Compounds 沙鼠抗氧化活性研究。萃取物及含硫化合物

Central European Journal of Biology Pub Date : 2021-12-14 DOI: 10.26650/eurjbiol.2021.1028656

B. Bayrak, R. Yanardağ

{"title":"Antioxidant Activities of Eremurus spectabilis M. Bieb. Extracts and Sulfur Compounds","authors":"B. Bayrak, R. Yanardağ","doi":"10.26650/eurjbiol.2021.1028656","DOIUrl":"https://doi.org/10.26650/eurjbiol.2021.1028656","url":null,"abstract":"Objective: Eremurus spectabilis M. Bieb. is grown in East and Southeast Mediterranean regions of Turkey, and is commonly used as an edible plant in these regions. The aim of the current study was to determine the antioxidant activities of different E. spectabilis M. Bieb. extracts and some sulfur compounds. Materials and Methods: In this study, the antioxidant activities of aqueous, ethanolic, and ethyl acetate extracts of E. spectabilis M. Bieb. and sulfur compounds (α-lipoic acid, cysteamine, cysteine, diallyl sulfide, glutathione, homocysteine, N-acetyl cysteine, vitamin U and 1,4-dithioerythritol) were assessed in several antioxidant tests. These tests included reducing power, cupric ion reducing antioxidant capacity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging, 2,2-diphenyl-1-picrylhydrazyl radical scavenging, N,N-dimethyl-4-phenylenediamine dihydrochloride radical scavenging, superoxide anion radical scavenging, hydroxyl radical scavenging, metal chelating activities and ferric ion reducing antioxidant power. The phytochemical profiles and total phenolic and flavonoid contents of all extracts were investigated. Results: It was found out that the antioxidant activities of all the extracts and sulfur compounds generally increased with concentration increases, the highest antioxidant activity was found in ethyl acetate extract, while the aqueous extract was found to have the lowest antioxidant activity. Diallyl sulfide was found to have the highest antioxidant activity, whereas vitamin U had the lowest antioxidant activity. It was observed that all extracts of E. spectabilis M. Bieb. and the sulfur compounds showed antioxidant activity. Conclusion: The E. spectabilis M. Bieb. and sulfur compounds investigated in this study could be considered as a source of antioxidants.","PeriodicalId":9711,"journal":{"name":"Central European Journal of Biology","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89262922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic Analysis Revealed Underlying Biological Pathways Associated with Hormetic Response of Hormone Positive Breast Cancer Cell Line Exposed to Low-Dose Flavonoid Mixture 蛋白质组学分析揭示了暴露于低剂量类黄酮混合物的激素阳性乳腺癌细胞系辐照反应的潜在生物学途径

Central European Journal of Biology Pub Date : 2021-12-09 DOI: 10.26650/eurjbiol.2021.1017807

M. Tüzüner, A. Ceviz

引用次数: 0

The Activation of PI3K/AKT/mTOR Signaling Pathway in Response to Cabazitaxel Treatment in Metastatic Castration-Resistant Prostate Cancer Cells 卡巴他赛治疗对转移性去势抵抗前列腺癌细胞PI3K/AKT/mTOR信号通路的激活

Central European Journal of Biology Pub Date : 2021-12-07 DOI: 10.26650/eurjbiol.2021.1018938

I. Eryilmaz, G. Güney Eskiler, Ceyda Çolakoğlu, Ü. Egelí, G. Cecener

引用次数: 0

Antitumoral Properties of a Pincer-Type Isonicotinohydrazone-Hg(II) Complex 钳型异烟碱腙-汞(II)配合物的抗肿瘤特性

Central European Journal of Biology Pub Date : 2021-12-07 DOI: 10.26650/eurjbiol.2021.1010029

Burcu Saygıdeğer Demir, Aycan Sezan, Ezgi Derinöz, E. Nas, Mehmet Özerten, G. Mahmoudi, Y. Saygıdeğer

引用次数: 0

Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats 苦瓜醇提物对醋酸致溃疡性结肠炎大鼠的治疗作用

Central European Journal of Biology Pub Date : 2021-12-06 DOI: 10.26650/eurjbiol.2021.1016222

Dilek Özbeyli, A. Şen, A. Aykaç, K. Teralı, Ö. Ç. Çilingir Kaya, Ismail Senkardes, G. Şener

引用次数: 0