Shi yan sheng wu xue bao最新文献_第9页

[Analysis of genetic diversity of 14 species of Cymbidium based on RAPDs and AFLPs]. [基于rapd和aflp的14种蕙兰遗传多样性分析]。

Shi yan sheng wu xue bao Pub Date : 2004-12-01

Hui Zhong Wang, Yu Dong Wang, Xiao Yun Zhou, Qi Cai Ying, Kang Le Zheng

引用次数: 0

[The investigation of Syn and NPY expression in brain tissues of diabetic model rat induced by streptozotocin]. 链脲佐菌素对糖尿病模型大鼠脑组织Syn和NPY表达的影响

Shi yan sheng wu xue bao Pub Date : 2004-12-01

Xiao Ming Zhang, Shu Han, Liang Zhou

引用次数: 0

[Determination of amylose content in transgenic progenies containing an antisense waxy gene and identifying of pure lines in indica rice]. [含反义蜡质基因转基因后代直链淀粉含量的测定及籼稻纯种系的鉴定]。

Shi yan sheng wu xue bao Pub Date : 2004-12-01

Ge Zhi Shen, Xin Qi Wang, Li Qing Yin, Hui Mei Wang

{"title":"[Determination of amylose content in transgenic progenies containing an antisense waxy gene and identifying of pure lines in indica rice].","authors":"Ge Zhi Shen, Xin Qi Wang, Li Qing Yin, Hui Mei Wang","doi":"","DOIUrl":"","url":null,"abstract":"Through genetic transformation mediated by Agrobacterium tumefaciens, an antisense waxy gene was introduced into Longtefu B line, a male sterile maintainer line in indica (Oryza sativa L.). Thirty transgenic plants showed integration of antisense waxy gene into the genome as determined by PCR assay, and twenty eight were confirmed by Southern blotting. T1 seeds from twenty one transgenic plants showed a marked decrease of amylose content, ranging from 3%-13% less than control, and seeds from some transgenic lines exhibited typical waxy phenotype. Six transgenic lines were selected to examine the amylose content in different generations. In the T4 generation, two homozygous lines, Long 3-1-1-1 and Long 5-8-2-1, were selected, with amylose content of 15.9% and 8.4% respectively. The amylose content in these lines is in consistent with the decrease of the accumulation of Wx protein as determined by SDS-PAGE electrophoresis. Cross and subsequent backcross of Long 3-1-1-1 and Long 5-8-2-1 with the Longtefu mare sterile line were performed to determine the changes of amylose content in F1 and B1F1 seeds. The results showed an average amlyose content of 21.4% in F1 seeds with Long 3-1-1-1 as a parent, while only 13.6% with Long 5-8-2-1 as a parent. In addition, the average amylose contents in B1F1 seeds were 17.1% and 9.3% respectively. Our results indicated that during the fertility transfer in the male sterile line, stable transgenic lines with medium or low contents of amylose had direct effects on amylose content in F1 seeds.","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"37 6","pages":"429-36"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25022896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Function of auxin-binding protein gene during cucumber fruit development]. [生长素结合蛋白基因在黄瓜果实发育中的作用]。

Shi yan sheng wu xue bao Pub Date : 2004-12-01

Ji Gang Bai, Xiu Juan Wang, Qian Xun Yin, Yu Zhen Xu, Rui Xue Zhao

引用次数: 0

[Involvement of nitric oxide in the effect of interleukin-2 on the contractility of rat ventricular cardiomyocytes]. 一氧化氮参与白细胞介素-2对大鼠心室心肌细胞收缩性的影响。

Shi yan sheng wu xue bao Pub Date : 2004-12-01

Hui Ping Wang, Qiang Xia, Chun Mei Cao, Yuan Lu

{"title":"[Involvement of nitric oxide in the effect of interleukin-2 on the contractility of rat ventricular cardiomyocytes].","authors":"Hui Ping Wang, Qiang Xia, Chun Mei Cao, Yuan Lu","doi":"","DOIUrl":"","url":null,"abstract":"The aim of the present study was to investigate the effect of interleukin-2 (IL-2) on the contractility in cardiomyocytes and the underlying mechanisms. Ventricular myocytes were isolated from adult male Sprague-Dawley rats. Contractile responses were evaluated by use of the video tracking system. Contractile parameters in cardiomyocytes electrically stimulated at 0.2 Hz included peak velocity of cell shortening (+dL/dtmax), peak velocity of cell relengthening (-dL/dtmax), contractile amplitude (dL), and end-diastolic cell length. Calcium transients of ventricular myocytes were determined by the spectrofluorometric techniques. Dose-dependent inhibition in + dL/dtmax, -dL/dtmax, dL and end-diastolic cell length were induced by IL-2 at 2-1000 U/ml. Pretreatment with the nitric oxide synthase inhibitor N(w)-nitro-L-arginine methyl ester (L-NAME, 100 micromol/L) and soluble guanylyl cyclase inhibitor 1H-[1,2,4]oxadiazolo [4,3a]quinoxalin-1-one (ODQ, 10 micromol/L) attenuated IL-2-induced inhibition of contractility. Aminoguanidine, an inhibitor of inducible nitric oxide synthase, had no effect on the inhibition by IL-2. IL-2 at 200 U/ml decreased the amplitude of electrically induced [Ca2+]i transients of ventricular myocytes. Pretreatment with ODQ diminished IL-2-induced inhibition of amplitude of the calcium transient. In conclusion, the present study indicates a direct action of IL-2 on cardiomyocyte contraction, possibly through an increased NO production, activation of soluble guanylyl cyclase and inhibition in intracellular Ca2+ level.","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"37 6","pages":"507-12"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25023355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Pattern of plasma sex steroid hormone levels during the breeding season of male and female skink: Eumeces chinensis]. [雌雄石龙子繁殖季节血浆性类固醇激素水平变化规律:中国石龙子]。

Shi yan sheng wu xue bao Pub Date : 2004-12-01

Jian Rao Hu, Ji Zeng Du, Xiang Ji

{"title":"[Pattern of plasma sex steroid hormone levels during the breeding season of male and female skink: Eumeces chinensis].","authors":"Jian Rao Hu, Ji Zeng Du, Xiang Ji","doi":"","DOIUrl":"","url":null,"abstract":"Changes in gonadal activity and plasma sex steroid hormone levels in male and female Eumece chinensis during the breeding season were described. The results showed that: The vitellogensis of follicles of female Eumeces chinensis needed the stimulation of 17beta-estradiol (E2). As ovary masses reached peak values between late April and mid-May, E2 levels rose to the top value by late March, and then sharply declined but went up again before preovulation; The physiological functions of plasma progesterone (P) consisted in its oviductal egg retention, embryo development, and eggshell formation. P levels fluctuated near the basic value between mid-March and late April. In mid-May, with the onset of ovulation, plasma P levels rose rapidly, reached peak value by late May and declined sharply after ovulation. Plasma E2 levels declined as plasma P levels rose, showing an inverse relationship between them; In males, plasma Testosterone (T) levels were closely correlated with the maintenance of spermatogenesis activities, male and male combat, sexual display, and mating. Plasma T levels tended to rise after the termination of hibernation, and reached peak value by mid-April. After mid-May, with the testis aggressing, plasma T levels gradually went down and reached bottom value by late June.","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"37 6","pages":"443-8"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25024043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The evolutionary traits of mitocondrial ND4-ND4L in Drosophila melanogaster species group]. [果蝇种群线粒体ND4-ND4L的进化特征]。

Shi yan sheng wu xue bao Pub Date : 2004-12-01

Shao Liang Mou, Qing Tao Zeng, Yuan Huai Qian

引用次数: 0

[Establishment of high-yield suspension cell line of Curcuma zedoaria (Berg.) Rosc and study on the volatile oil synthesis-controlling with precursors]. 莪术高产悬浮细胞系的建立[j]。Rosc与前体控制挥发油合成的研究[j]。

Shi yan sheng wu xue bao Pub Date : 2004-12-01

Guan Lin Wang, Hong Mei Luo, Hong Jun Fang, Hai Dong Wu, Yang Song

引用次数: 0

[Isolation and prelimary characterization of a kind of rat liver potential progenitor cells small round hepatic cells]. [一种大鼠肝潜能祖细胞小圆肝细胞的分离及初步表征]。

Shi yan sheng wu xue bao Pub Date : 2004-12-01

Zhong Yu Yan, Wei An, Ping Wang, Ji Dong Jia

{"title":"[Isolation and prelimary characterization of a kind of rat liver potential progenitor cells small round hepatic cells].","authors":"Zhong Yu Yan, Wei An, Ping Wang, Ji Dong Jia","doi":"","DOIUrl":"","url":null,"abstract":"Liver undergoes profound regeneration usually after hepatic damage. It has been shown in recent study that two kinds of liver stem cells, which are mainly oval cells (OVCs) and small hepatocytes, are involved in the process of liver regeneration as they differentiated into premature liver cells. However, the origination of oval cells as well as its differentiation property is not quite understood. In this study, we isolated a novel potential liver progenitor cells, namely small round cells (SRCs). The cellular features of the cells such as morphological appearance, surface marker, growth curve, and differentiation induced by DMSO were analyzed. SRCs and OVCs were obtained by using discontinuous digestions and isopyknic centrifugation, respectively. With non-radioactive measurement of MTT, the cell growth was assayed. Meanwhile, SRCs were stained with antibodies against CK19, ALB and AFP antibody to characterize their tissue-specificities. The results showed that SRCs appeared in round-shaped, but irregular in size. The nuclei of SRCs were relative small with rich nucleolus visible. SRCs were semi-floated during primary culture. Even cultured in F12:DMEM (1:1) mixed-medium supplemented with 15% fetal calf serum, viability of the cells could merely be expanded over 7 days. SRCs were positively stained by CK19, ALB and AFP within initial 3 days. After DMSO stimulation, SRCs were not only morphologically changed into OVCs, but also expressed those markers identical to OVCs. It was suggested that SRCs could be considered as an potential candidate of liver progenitors which were related to OVCs.","PeriodicalId":77395,"journal":{"name":"Shi yan sheng wu xue bao","volume":"37 6","pages":"475-81"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25024048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Suppression of apple polyphenol oxidase by double-stranded RNA (RNAi)]. [双链RNA (RNAi)抑制苹果多酚氧化酶]。

Shi yan sheng wu xue bao Pub Date : 2004-12-01

Yan Hong Cao, Zhen Zhang, Quan Hong Yao, Ri He Peng, Ai Sheng Xiong, Xian Li

引用次数: 0