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Effect of cellulose acetate 0.2 micron filter on platelet specific proteins plasma levels. 醋酸纤维素0.2微米过滤器对血小板特异性蛋白血浆水平的影响。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
F Stevanato, R Paolini, F Fabris, A Casonato, G Cella
{"title":"Effect of cellulose acetate 0.2 micron filter on platelet specific proteins plasma levels.","authors":"F Stevanato,&nbsp;R Paolini,&nbsp;F Fabris,&nbsp;A Casonato,&nbsp;G Cella","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We are reporting the effect of a cellulose acetate 0.20 micron filter (Flow Pore D26) on preparation of platelet poor plasma (PPP) for subsequent assay of platelet specific proteins. PPP was obtained using the Edinburgh anticoagulant mixture and a centrifugation at 2,300 g for 20 minutes. The mean levels of platelet factor 4 (PF4) and beta-thromboglobulin (beta TG) before filtration were: PF4 15.7 ng/ml (1.5-42.8 ng/ml); beta TG 62.0 ng/ml (25-140 ng/ml). After filtration we obtained: PF4 4.8 ng/ml (1.0-8.9 ng/ml), beta TG 28.4 ng/ml (8.5-51.1 ng/ml). Thus difference for both the platelet specific proteins was statistically significant (p less than 0.005). The contamination by platelet-like material was also greatly affected by the filter (before filtration 4.3 x 10(6)/ml), (2-15 x 10(6)/ml), after filtration 2.0 x 10(6)/ml (1-5 x 10(6)/ml), p less than 0.005). If a high speed centrifuge is not available, the use of this simple filter method could avoid falsely elevated values of platelet specific proteins.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 3","pages":"377-81"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12867498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Laser nephelometer evaluation of factor IX. 激光浊度计评价因子IX。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
G Ruzza, A R Lazzaro, M L Viero, A Girolami
{"title":"Laser nephelometer evaluation of factor IX.","authors":"G Ruzza,&nbsp;A R Lazzaro,&nbsp;M L Viero,&nbsp;A Girolami","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Laser Nephelometry is a technique which allows the evaluation of the concentration of several serum proteins and clotting factors. By means of this technique it is also possible to study the kinetic of the reaction between antigen and antibody. In a few instances the method was also applied in the characterization of abnormal molecules. We developed assays for the measurement of Factor IX antigen and the results were compared with those obtained by conventional immunological methods such as rocket immunoelectrophoresis. Plasmas from patients with haemophilia B, on coumarin treatment, with liver cirrhosis were studied. A standard reference curve was obtained using pooled normal plasma. The factor IX levels obtained by laser nephelometer correlated fairly well with those obtained by electroimmunoassay.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 6","pages":"843-9"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12878093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[One-year analysis of bleeding events in 223 hemophiliacs in the DDR]. [DDR 223例血友病患者一年出血事件分析]。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
G Syrbe, P Linde
{"title":"[One-year analysis of bleeding events in 223 hemophiliacs in the DDR].","authors":"G Syrbe,&nbsp;P Linde","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The reports of 223 haemophiliacs living in the districts Halle, Leipzig, Karl-Marx-Stadt, and Gera had been analyzed for bleeding events in 1986. There were suffering from haemophilia A 185, from haemophilia B 36 and 2 persons from a complex disorder. In each case 73 patients suffer from a severe and a moderate type of haemophilia. In 55 patients the diagnosis was mild form of haemophilia and in 22 subhaemophilia. Altogether 1802 bleeding episodes had been registered with the following locations: elbow joint 21.0%, knee joint 17.9%, ankle 14.2%, shoulder 4.3%. In 5% of all cases multiple joints had been affected at the same time. Soft tissue bleedings occurred in 16.7% of all cases. For substitution treatment of these 1802 bleeding events altogether 9020 transfusion units were needed, appropriate to 40.4 per year and 52.4 per year and bleeding patient respectively. Expense and duration of treatment depend on the bleeding location. Conclusions for the clinics and the transfusion service are possible.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 4","pages":"519-25"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12878784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of recombinant human alpha interferon (INF) on chronic granulocytic leukaemia (CGL) clonogenic growth of bone marrow haematopoietic progenitors. 重组人α干扰素(INF)对慢性粒细胞白血病(CGL)骨髓造血祖细胞克隆生长的影响
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
A Zduńczyk, H Miszta, Z Dabrowski
{"title":"Effect of recombinant human alpha interferon (INF) on chronic granulocytic leukaemia (CGL) clonogenic growth of bone marrow haematopoietic progenitors.","authors":"A Zduńczyk,&nbsp;H Miszta,&nbsp;Z Dabrowski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of recombinant alpha interferon (INF) to the colony stimulating factor (CSF) production was examined with in vitro culture of the bone marrow of patients with chronic granulocytic leukaemia (CGL). It could be found that addition of interferon into a suspension of preincubated phytohaemagglutinin (PHA) lymphocytes from peripheral blood represents an inhibity factor for colony and cluster formation in autologic human marrow cultures.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 1","pages":"45-50"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12859762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Examination of the differential hematocrit and the plasma viscosity under the influence of ionizing radiation]. [电离辐射影响下红细胞压积差和血浆粘度的检查]。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
J Thiel, H R Böhme, R Melzer, W Prager
{"title":"[Examination of the differential hematocrit and the plasma viscosity under the influence of ionizing radiation].","authors":"J Thiel,&nbsp;H R Böhme,&nbsp;R Melzer,&nbsp;W Prager","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Systematic investigations into the impact of haemorheological parameters through ionizing rays are not available in literature. The main factors influencing the flow capacity of the blood were determined in vitro by using a set of special methods. Even by using high dosages of irradiation (600 R) only those changes in the flow capacity of the blood could be identified which lay in or near the dispersion area of the methods. These investigations carried out in vitro only take into account the immediate effect of the blood and not rheological changes caused by the intact organism after irradiation.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 1","pages":"7-14"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12859766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Myelodysplastic syndromes: analysis of 51 cases. Therapy with low doses of arabinosyl cytosine of leukaemic transformation. 骨髓增生异常综合征51例分析。低剂量阿拉伯糖胞嘧啶治疗白血病转化。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
A Candido, P L Rossi, R Mancini, G Alfano, R Tartaglione, G Menichella, G Mango
{"title":"Myelodysplastic syndromes: analysis of 51 cases. Therapy with low doses of arabinosyl cytosine of leukaemic transformation.","authors":"A Candido,&nbsp;P L Rossi,&nbsp;R Mancini,&nbsp;G Alfano,&nbsp;R Tartaglione,&nbsp;G Menichella,&nbsp;G Mango","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Clinical, haematological, cytogenetic features and therapeutic problems of 51 patients with MDS were examined. Patients were distributed in 5 FAB subgroups: RA 21, SA 7, RAEB 8, RAEB-t 9 and MMCL 6 patients. Leukaemic transformation occurred in 3 RA, 3 RAEB, 7 RAEB-t and 3 MMCL patients. No SA patient suffered from leukaemic transformation. Cytogenetic alterations occurred in 13 of 29 examined patients; 5q- was the most common abnormality. We did not find any relation between chromosomal anomalies and FAB subgroups. Leukaemic transformation, however, was more frequent in patients with cytogenetic aberrations. In some cases it was not easy to determine the precise diagnostic allocation according to FAB subgroups; it is possible, however, to subdivide MDS prognosis into 2 classes. The more satisfactory therapy of leukaemic transformation is often due to low doses of Ara-C; this therapy allowed a better survival and sometimes to obtain CR which in a M6 ANLL patient continued for 24 months.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 1","pages":"95-104"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12859770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High dose ARA-C as induction therapy of acute myeloid leukaemia. 大剂量ARA-C诱导治疗急性髓性白血病。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
H Minigo, D Nemet, A Planinc-Peraica, V Bogdanic, B Labar, B Jaksic, E Hauptmann
{"title":"High dose ARA-C as induction therapy of acute myeloid leukaemia.","authors":"H Minigo,&nbsp;D Nemet,&nbsp;A Planinc-Peraica,&nbsp;V Bogdanic,&nbsp;B Labar,&nbsp;B Jaksic,&nbsp;E Hauptmann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Twenty-one newly diagnosed adult AML patients were treated with high dose Ara-C (HD-ARA-C) as a single induction treatment with the dose of 2 g/m2 q 12 hours x 12. Seventy-six percent (16/21) responded with complete remission. Three patients died in induction in pancytopenia, another one died on day 44 in CR due to bleeding of pulmonary aspergilloma. This treatment seems to be highly efficient for remission induction, but requires an adequate transfusion and other supportive measures. The overall toxicity was transient and seems acceptable. However, the remission duration is unacceptably short, the consolidation with the same treatment seems to be inadequate. More aggressive treatment in postinduction period seems to be warranted.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 1","pages":"135-40"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12859927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Acquired amegakaryocytic thrombocytopenic purpura (AATP): a study of autologous megakaryocyte progenitors and the effect of patients plasma on normal marrow megakaryocyte colony formation. 获得性无核细胞血小板减少性紫癜(AATP):自体巨核细胞祖细胞及患者血浆对正常骨髓巨核细胞集落形成影响的研究。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
M Podolak-Dawidziak
{"title":"Acquired amegakaryocytic thrombocytopenic purpura (AATP): a study of autologous megakaryocyte progenitors and the effect of patients plasma on normal marrow megakaryocyte colony formation.","authors":"M Podolak-Dawidziak","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Megakaryocyte progenitors (Colony Forming Unit-Megakaryocyte, CFU-Mk) and the effect of plasma on megakaryocyte colony formation in normal human marrow (Plasma Factor Index-Megakaryocyte, PFI-Mk) were studied in six patients with acquired amegakaryocytic thrombocytopenic purpura (AATP) and in ten normal subjects. Assay was based on the method of Messner. In one AATP marrow culture four CFU-Mk were found, in the other two a single CFU-Mk were present, and in the remaining three samples no megakaryocyte colonies were observed. PFI-Mk in AATP patients was significantly higher than in normal subjects. No correlation was found between PFI-Mk and platelet count in either group. The results of this study indicate the presence of an intrinsic defect at the level of CFU-Mk in AATP marrow. PFI-Mk in AATP patients relate to changes in marrow megakaryocyte number rather than to peripheral blood platelet count.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 2","pages":"347-51"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12862325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluctuation of thrombin-antithrombin III complex in patients with acute myocardial infarction: influence of low-dose heparin administration. 急性心肌梗死患者凝血酶-抗凝血酶III复合物波动:低剂量肝素给药的影响。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
P Psuja, K Lewandowski, Z Turowiecka, M Zozulińska, A Tokarz, K Zawilska
{"title":"Fluctuation of thrombin-antithrombin III complex in patients with acute myocardial infarction: influence of low-dose heparin administration.","authors":"P Psuja,&nbsp;K Lewandowski,&nbsp;Z Turowiecka,&nbsp;M Zozulińska,&nbsp;A Tokarz,&nbsp;K Zawilska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The haemostatic parameters were studied within 14 days of acute myocardial infarction (AMI) in 103 patients randomly allocated into a group receiving low-dose heparin or into a group treated without anticoagulants. Patients with isotopic evidence of deep vein thrombosis were excluded from the analysis. An important formation of thrombin-antithrombin III complex (TAT) in the plasma was detected in the early stage of the disease. It was accompanied by an activation of plasma intrinsic fibrinolysis (IF), an elevation of fibrinogen and its degradation products (FDP) and a reduction of extrinsic plasma fibrinolytic activity (EF) together with normal levels of factor X, antithrombin III (AT III), protein C and alpha-2-antiplasmin. Sequentially studies periods of the disease revealed a diminution of TAT complex concentration in the plasma on the seventh day of AMI together with a rise of the both plasma fibrinolytic activities (IF, EF) as well as an elevation of fibrinogen and its degradation products, returning to the initial values on the 14 day of AMI. In the patients treated with heparin the augmentation of TAT complex in the plasma was prolonged until the fifth day of AMI. Moreover, heparin administration was connected with significantly higher levels of AT III and protein C along with a lower concentration of factor X and FDP on the seventh day of the disease. The fluctuation of fibrinolytic activities (IF, EF) in the plasma was heparin-independent. The present results indicate that low-dose heparin treatment modulates the plasmatic fluctuation of TAT complex as well as factor X, AT III and protein C levels in patients with acute myocardial infarction.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 2","pages":"229-36"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12862347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Exclusion chromatography for the separation of cryoprotective agents from freeze-preserved blood cells]. [从冷冻保存的血细胞中分离冷冻保护剂的排除色谱法]。
Folia haematologica (Leipzig, Germany : 1928) Pub Date : 1990-01-01
L Mavrina, S König, A Klein, H Woehlecke, E Richter, G Matthes, R Ehwald
{"title":"[Exclusion chromatography for the separation of cryoprotective agents from freeze-preserved blood cells].","authors":"L Mavrina,&nbsp;S König,&nbsp;A Klein,&nbsp;H Woehlecke,&nbsp;E Richter,&nbsp;G Matthes,&nbsp;R Ehwald","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method for separating low molecular cryoprotectiva from freeze-conserved erythrocyte- and thrombocyte-concentrates by exclusion chromatography has been described. A new vesicular packing material has been used. Only 25 to 30 minutes are necessary in order to separate glycerol respectively dimethylsulphoxide (DMSO) completely from the cells. 86.5% of the erythrocytes and 75.4% of the thrombocytes were recovered after the separation process.</p>","PeriodicalId":75853,"journal":{"name":"Folia haematologica (Leipzig, Germany : 1928)","volume":"117 2","pages":"251-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12862348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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