Clinical complementary medicine and pharmacology最新文献

{"title":"Epigenetic and Transcriptional Modulator Potential of Epigallocatechin-3-gallate and Genistein on Fetal Hemoglobin Reactivators Genes","authors":"Edna Ribeiro ,&nbsp;Mariana Delgadinho ,&nbsp;Elisabete Matos ,&nbsp;Raquel Santos ,&nbsp;Daniela Sousa ,&nbsp;Heloísa Galante ,&nbsp;Miguel Brito","doi":"10.1016/j.ccmp.2022.100034","DOIUrl":"10.1016/j.ccmp.2022.100034","url":null,"abstract":"<div><h3>Background</h3><p>β-hemoglobinopathies are one of the most common recessive genetic diseases worldwide, with limited treatments available, particularly in developed countries where the prevalence is higher. Pharmacological reactivation of Fetal Hemoglobin (HbF) is a promising therapeutic strategy. However, approximately 25% of the patients do not respond to Hydroxyurea (HU), the first and most commonly used HbF inducing agent approved by the FDA.</p></div><div><h3>Objective</h3><p>Here, we performed an <em>in vitro</em> assessment of transcriptional effects induced by natural bioactive compounds, namely Epigallocatechin-3-gallate (EGCG) and genistein (GN) in globin genes (<em>HBA1, HBB, HBG1</em> and <em>HBG2)</em> in HbF regulators/silencer genes (<em>KLF1, BCL11A, MYB</em> and <em>BGLT3)</em> and in epigenetic regulator genes (<em>DNMT1, DNMT3A, DNMT3B, HDAC1, HDAC2, HDAC3</em> and <em>HDAC8</em>)<em>.</em> Moreover, we evaluated EGCG’s <em>in vivo</em> effects in hematological parameters of healthy volunteers.</p></div><div><h3>Methods</h3><p>K562 cells were exposed for 72 and 96 h to GN and EGCG at 100, 250 and 500 ng/mL. Cell proliferation and viability were measured, and transcriptional levels were evaluated by qRT-PCR. For <em>in vivo</em> assay, complete blood count was determined by flow cytometry and HbF level was determined through HPLC in 30 healthy individuals before and after 225 mg EGCG ingestion per day during a 90-day period.</p></div><div><h3>Results</h3><p>Both compounds impact cellular metabolism and proliferation with no cytotoxic effects. Divergent GN and EGCG effects in globin and <em>BGLT3</em> expression levels suggest the involvement of divergent signaling pathways. As for the epigenetic potential, EGCG particularly affects <em>HDAC2</em> and <em>HDAC8</em> transcription, whereas GN significantly affects expression patterns of methylation and acetylation modulators. HU appears to have time divergent effects, with greater impact in methylation at 72 h (overregulates <em>DNTM3A</em>) while affecting acetylation mostly at 96 h (downregulates <em>HDAC1</em> and <em>HDAC8</em>). Additionally, <em>in vivo,</em> EGCG demonstrated a modulator effect in hematopoiesis and HbF induction.</p></div><div><h3>Conclusion</h3><p>Our results advocate EGCG and GN with HbF pharmacological reactivation potential and sustain further research as new alternative approaches for β-hemoglobinopathies therapies.</p></div>","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"2 2","pages":"Article 100034"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772371222000158/pdfft?md5=53d070631d0f96249055a890292d1008&pid=1-s2.0-S2772371222000158-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42651328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Chinese Medicine Constitution and Clinical Data Association with Machine Learning for Prediction of Spontaneous Abortion","authors":"Yan Liu ,&nbsp;Yangyang Geng ,&nbsp;Liuqing Yang ,&nbsp;Shate Xiang ,&nbsp;Qiaotong Wang ,&nbsp;Lanyawen Hu ,&nbsp;Ping Ye","doi":"10.1016/j.ccmp.2021.100016","DOIUrl":"10.1016/j.ccmp.2021.100016","url":null,"abstract":"<div><h3>Background</h3><p>Early prevention of Spontaneous Abortion (SA) is essential for the treatment of recurrent spontaneous abortion.</p></div><div><h3>Objective</h3><p>In this retrospective study, a variety of machine learning methods were used to develop predictive models and diagnose the potential risk of SA.</p></div><div><h3>Methods</h3><p>A total of 663 pregnant women participated in the case-control study, 586 of which were SA patients and 77 were normal parturition women. The research data included 25 features of Traditional Chinese Medicine (TCM) constitution and clinical data related to SA. This work utilized 8 machine learning techniques including logistic regression, gradient boosting decision tree, k-nearest neighbor, classification and r-egression tree, multilayer perceptron, support vector machine, random forest and XG-Boost to predict SA. The performances of the applied models were evaluated by using the method of 10-fold cross-validation and by computing the diagnostic test characteristics, including accuracy, precision, recall, <span><math><msub><mi>F</mi><mn>1</mn></msub></math></span> score, and the AUC of ROC curve.</p></div><div><h3>Results</h3><p>The <span><math><msub><mi>F</mi><mn>1</mn></msub></math></span> scores of these eight machine learning techniques were all above 97.5%. Among them, gradient boosting decision tree had the best prediction result on SA. The accuracy, precision, recall, <span><math><msub><mi>F</mi><mn>1</mn></msub></math></span> score, and the AUC of ROC curve of gradient boosting decision tree were 97.9%, 99%, 98.6%, 98.8%, and 97.3%, respectively.</p></div><div><h3>Conclusion</h3><p>The paper has accurately predicted the risk of SA combined with TCM constitution and clinical data.</p></div>","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"2 2","pages":"Article 100016"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772371221000164/pdfft?md5=d4ab712a1e67bf47d86b9212ea8cd90c&pid=1-s2.0-S2772371221000164-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41755009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture Alleviates Anxiety-like Behavior in Pain Aversion Rats by Attenuating the Expression of Neuropeptide Y in Anterior Cingulate Cortex","authors":"Fangbing Shao ,&nbsp;Junying Du ,&nbsp;Sisi Wang ,&nbsp;Rok Cerne ,&nbsp;Junfan Fang ,&nbsp;Xiaomei Shao ,&nbsp;Xiaoming Jin ,&nbsp;Jianqiao Fang","doi":"10.1016/j.ccmp.2022.100019","DOIUrl":"10.1016/j.ccmp.2022.100019","url":null,"abstract":"<div><h3>Background</h3><p>Pain is considered as a multidimensional conscious experience that includes a sensory component and a negative affective-motivational component. Electroacupuncture (EA) is widely used to treat pain and pain-induced negative emotions, however, little is known about the mechanisms underlying the effect of EA.</p></div><div><h3>Objective</h3><p>This study investigated the effect of EA on alleviating the anxiety-like behaviors in pain aversion rats and its anterior cingulate cortex (ACC) regulation mechanism.</p></div><div><h3>Methods</h3><p>After a Freund's complete adjuvant (CFA)-conditioned place aversion (C-CPA) model was established in rats, EA treatment (2/100 Hz, 30 min, once/day, 4 days totally) was applied at bilateral Zusanli (ST36) and Kunlun (BL60) acupoints. Von Frey filaments were used to measure changes of pain withdrawal threshold (PWT) at indicated time points. Elevated zero maze (EZM) was used to investigate the changes of pain-related anxiety and CPA was used to investigate the changes of pain aversion. The protein expression levels of GAD67, PV, and NPY in ACC were detected by Western blotting.</p></div><div><h3>Results</h3><p>Compared with the control group, the staying time in the \"CFA-paired compartment\" was significantly reduced, and the PWT was decreased in model group. In the EZM assessment, the distance and the time in open arm, as well as the number of open arm entries of model group were significantly lower than those in the control group. In the CPA assessment, the time spent in the \"CFA-paired compartment\" was significantly decreased in model group compared with control group, and EA reversed the changes in pain sensation and in pain-related emotions. Western blotting showed that the NPY level, but not the levels of GAD67 and PV, was significantly increased in the ACC of the model group compared to that of the control group. The increased expression of NPY in the ACC was significantly downregulated by EA, while sham EA produced no such effect.</p></div><div><h3>Conclusion</h3><p>EA can effectively relieve the pain and pain-related emotions, and its mechanism may be achieved by down-regulating the expression of NPY in the ACC.</p></div>","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"2 2","pages":"Article 100019"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772371222000018/pdfft?md5=a90dee5d003f849c9dcc7acba67e0879&pid=1-s2.0-S2772371222000018-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47226326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"METASTATIC LESION OF THE BRAIN. DIAGNOSIS AND TREATMENT","authors":"M. Bobkov","doi":"10.12737/2409-3750-2022-8-1-26-32","DOIUrl":"https://doi.org/10.12737/2409-3750-2022-8-1-26-32","url":null,"abstract":"The article provides an overview of the current literature on the diagnosis and treatment of second-ary (metastatic) damage to the brain substance. \u0000Over the past years, Russia has seen a high incidence of malignant neoplasms, with a clearly observed upward trend. In 2019, this figure was 376.3 per 100,000 population compared to 369.6 per 100,000 population in 2018 and 293.7 per 100,000 population in 2000. However, thanks to modern methods of diagnosis and treatment, the life span of patients suffering from malignant neo-plasms is significantly increased, and, as a result, the chance of “surviving” before the onset of a metastatic lesion of the brain substance increases. Today, this formidable complication of the tumor process occurs in approximately 8-10% of cancer patients, while in most of them they are symptomatic. In some nosological forms of malig-nant neoplasms (small cell lung cancer, melanoma), the frequency of brain metastasis can reach 80% or more.","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76943317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BREAST CANCER: general questions, clinic, diagnosis","authors":"A. Guseynov, T. Guseynov, M. Bobkov, V. Blincov, V. Odincov","doi":"10.12737/2409-3750-2022-8-1-5-13","DOIUrl":"https://doi.org/10.12737/2409-3750-2022-8-1-5-13","url":null,"abstract":"The lecture provides up-to-date information for doctors of various specialties: oncologists, surgeons, mammologists, general practitioners on breast cancer problems. The issues of etiology and pathogenesis, classification, clinic of breast tumors are highlighted, methods of modern diagnosis and differential diagnosis of breast cancer are described in detail.","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79622707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic physical culture as a method of prevention of cardiovascular diseases","authors":"É. Asadova","doi":"10.12737/2409-3750-2022-8-1-22-25","DOIUrl":"https://doi.org/10.12737/2409-3750-2022-8-1-22-25","url":null,"abstract":"The influence of physical culture on the health of the cardiovascular system (CCC) and the possibility of using physical therapy (exercise therapy) for the prevention of cardiovascular diseases (CVD) are considered. For the prevention of CCC diseases, physical education is recommended for everyone, but especially for people with risk factors. Various forms of classes can be used. Exercise therapy for CVD has a powerful positive effect on the patient's body, improves physical and mental condition, reduces the risk of many diseases and helps to recover from them, regulates the body, increases activity and efficiency.","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"125 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76797372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dermatomyositis resistant to therapy: Demonstration of a clinical case.","authors":"A. Fleyshman","doi":"10.12737/2409-3750-2022-8-1-14-18","DOIUrl":"https://doi.org/10.12737/2409-3750-2022-8-1-14-18","url":null,"abstract":"Dermatomyositis is an autoimmune disease characterized by damage to proximal symmetrical muscle groups, skin in the form of erythema, as well as other systems. With timely diagnosis, dermatomyositis responds well to therapy with high doses of glucocorticosteroids. However, serious complications may develop during the course of the disease, for example, aspiration pneumonia, which significantly affect the patient's management tactics and the course of the disease. This article presents a clinical case of aspiration pneumonia on the background of dermatomyositis, which significantly limited the arsenal of therapy used.","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"430 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76645077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CLINICAL OBSERVATION OF STILL'S DISEASE","authors":"A. Morozova","doi":"10.12737/2409-3750-2022-7-4-22-27","DOIUrl":"https://doi.org/10.12737/2409-3750-2022-7-4-22-27","url":null,"abstract":"A clinical case of Still's disease in an 18-year-old girl who debuted with sore throat, lymphadenopathy, sore throat, febrile fever up to 39 ° C and maculopopular rash at its height is presented. The process of diagnosis, the appearance of symptoms over time and the diagnosis by exclusion is demonstrated.","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79091489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Goodpascher syndrome: a clinical case","authors":"L. Zhuravleva","doi":"10.12737/2409-3750-2022-7-4-28-31","DOIUrl":"https://doi.org/10.12737/2409-3750-2022-7-4-28-31","url":null,"abstract":"Goodpasture syndrome refers to systemic vasculitis with small-caliber vascular lesions, immunocomplex vasculitis. Immunocomplex vasculitis - vasculitis with moderate or pronounced deposits of immunoglobulins and/or complement components in the vascular wall, mainly small vessels (capillaries, venules, arterioles and small arteries). Glomerulonephritis is often observed. Diagnosis is difficult due to the rare frequency of the disease. The article presents a clinical case of Goodpascher syndrome.","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81254682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The problem of coexistence of the overlap syndrome and the neoplastic process on the example from clinical practice","authors":"O. Blashkevich","doi":"10.12737/2409-3750-2022-7-4-17-21","DOIUrl":"https://doi.org/10.12737/2409-3750-2022-7-4-17-21","url":null,"abstract":"The publication considers a case of ovarian malignancy in a patient suffering from 11 years of overlap syndrome: a combination of progressive systemic sclerosis with dermatomyositis (antisynthetic syndrome). Timely recognition and treatment of cancer prevented an unfavorable outcome for this patient. At the same time, the termination of the basic anti-inflammatory therapy of systemic diseases (in this case, the cancellation of mycophenolate mofetil due to the lack of the drug in the pharmacy network of the city) led to a deterioration of the course of the disease, an aggravation of the clinical picture, which can create a so-called \"vicious circle\" of these pathologies.","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73403498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}