{"title":"The Use of Multiplex PCR Reactions to Identification of Lactic Acid Bacteria of Dangke","authors":"Nurwilda Kaswi, M. Hatta, R. Sjahril","doi":"10.20527/jbk.v19i1.15723","DOIUrl":"https://doi.org/10.20527/jbk.v19i1.15723","url":null,"abstract":"Abstract: Lactic acid bacteria can be isolated from various types of milk including dairy products. Dangke is an Indonesian traditional cheese made by Enrekang community, South Sulawesi. This study aims to identify the lactic acid bacteria by multiplex PCR method which are isolated from dangke obtained from Enrekang Regency. This type of research used descriptive research with laboratory observation methods was performed on 30 cups of dangke are obtained from the markets in 5 sub-districts producing dangke in Enrekang Regency: they are the sub-districts of Cendana, Enrekang, Anggeraja, Alla, and Baraka. The results showed that Lactobacillus acidophilus was the most identified (33.33%) compared to Lactobacillus plantarum (16.67%). Based on the results of the research that has been carried out, it can be concluded that the lactic acid bacteria have been identified in dangke samples obtained from several sub-districts producing dangke in Enrekang Regency are Lactobacillus acidophilus and Lactobacillus plantarum species. Thus, we suggest that this laboratory technique is used to confirm the various species of lactic acid bacteria so it can prove more rapid and exactly that dangke is the rich content of lactic acid bacteria as probiotic candidate.","PeriodicalId":53378,"journal":{"name":"Berkala Kedokteran","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46157544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effectiveness of Dexamethasone, Methylprednisolone and Hydrocortisone Administration on LDH, CRP, D-and PaO2/FiO2 Ratio","authors":"M. Zubaidi, M. Isa, I. Nurrasyidah","doi":"10.20527/jbk.v19i1.15708","DOIUrl":"https://doi.org/10.20527/jbk.v19i1.15708","url":null,"abstract":"Abstract: Steroids are the standard recommended therapy for COVID-19, especially in critically ill patients. Previous studies have shown that steroids are beneficial for inflammatory markers such as C-Reactive Protein (CRP), Lactate Dehydrogenase (LDH) and D-dimer as well as the value of the PaO2/FiO2 ratio. This study aims to determine the differences in the effects of the steroid’s dexamethasone, methylprednisolone and hydrocortisone on inflammatory markers. This research is a retrospective cohort study. The samples were all COVID-19 patients with critical degrees from January to June 2021. The independent variables were dexamethasone, methylprednisolone and hydrocortisone. The dependent variables are CRP, LDH, D-dimer and the ratio of PaO2/FiO2. The statistical test used was the Kolmogorov-Smirnov test and continued with the Kruskal-Wallis test and the Mann-Whitney U test. A total of 148 subjects with a total sample of dexamethasone 60, methylprednisolone 55 and hydrocortisone 33. The statistical test results showed a significant difference in the effect of the steroid dexamethasone, methylprednisolone and hydrocortisone on the value of the PaO2/FiO2 ratio (p=0.04). Administration of hydrocortisone and dexamethasone was statistically superior to methylprednisolone for increasing the PaO2/FiO2 ratio.","PeriodicalId":53378,"journal":{"name":"Berkala Kedokteran","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43801694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Budiarti, S. Kaidah, Nur Adnia, Vania Puspitasari Sangadi
{"title":"Antibacterial Activity of Mixed Infusion of Averrhoa bilimbi L. Fruit and Cananga odorata Flowers Against Several Numbers of Bacterial Colonies in Vitro Test","authors":"L. Budiarti, S. Kaidah, Nur Adnia, Vania Puspitasari Sangadi","doi":"10.20527/jbk.v19i1.15705","DOIUrl":"https://doi.org/10.20527/jbk.v19i1.15705","url":null,"abstract":"Abstract: Antiseptic alcohol 70% is known to be effective in inhibiting the growth of bacterial colonization on the hands, but its use in the long term can cause skin disorders. An alternative antiseptic can be made from a mixture of star fruit (Averrhoa bilimbi L.) and flower Cananga (Cananga odorata) which contains several antibacterial compounds. The purpose of this study was to analyze the antibacterial activity of the infusion mixture of Averrhoa bilimbi and Cananga odorata flowers in inhibiting the growth of Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Salmonella typhi, and Shigella sonnei bacteria. This in vitro research design used a posttest only with control group design with the observation parameter being the number of test bacteria colonies that grew on the observation medium. The treatments tested were a mixture of 50%, 75%, and 100% Averrhoa bilimbi infusion and 100% Cananga odorata, and 70% alcohol control. The results showed that the infusion mixture of Averrhoa bilimbi and Cananga odorata had different inhibitory effects on Gram-positive and Gram-negative bacteria; the number of colonies of Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli was not significantly different from the mixed treatment concentration of 100% (1:1) with 70% alcohol treatment. In conclusion, the infusion mixture of Averrhoa bilimbi fruit and Cananga odorata flower had antibacterial activity against Gram-positive and Gram-negative bacteria, and the best inhibitory effect was obtained in the 100% infusion mixture treatment.","PeriodicalId":53378,"journal":{"name":"Berkala Kedokteran","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43821308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Rice Bran’s Effect on Total Cholesterol, HDL, and LDL/HDL Ratio on Hypercholesterolemia Rats","authors":"E. Stujanna, S. Purwowiyoto, W. Sukarya","doi":"10.20527/jbk.v19i1.15729","DOIUrl":"https://doi.org/10.20527/jbk.v19i1.15729","url":null,"abstract":"Abstract: Hypercholesterolemia is a metabolism disorder characterized by increased or decreased lipid fraction in plasma. The of this research was to prove the effect of Rice bran to decrease of total Cholesterol rate, ratio of LDL / HDL and increase rate of HDL in hypercholesterolemia male white rats (Rattus norvegicus). The Research of the study was pure experiment by using post test randomized control design. Research Sample divided into five groups. The first group as a negative control, the second group as a positive control, and another groups were given rice brand, with various dose: 0,54 ml/a/day, 1,08 ml/a/day, 1,62 ml/a/day. The results of this research is rice brand can decrease of total cholesterol rate ( r2=0,964), decreased of ratio of LDL / HDL (r2=0,836), and increase of rate of HDL (r2=0,951) (ANOVA P=0,000). Rice bran can decrease total cholesterol rate, ratio of LDL / HDL and increase rate of HDL blood.","PeriodicalId":53378,"journal":{"name":"Berkala Kedokteran","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45638009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Flattening The Epidemic Curve from a Statistical and Epidemiological Approach in Malaysia","authors":"Nur Aifiah Ibrahim","doi":"10.20527/jbk.v19i1.15724","DOIUrl":"https://doi.org/10.20527/jbk.v19i1.15724","url":null,"abstract":"Abstract: Epidemic curves will help decision-makers and the public to give a picture and pattern of the outbreak. It will create a path to explore the epidemiology of Covid-19. One of the highlights is the onset of illness. One of the approaches is to track the progression of Covid-19 in Malaysia. In Malaysia, the numbers and figures began to convey a message about the epidemic progression and put in efforts to stop the pandemic. The disease pattern grows exponentially. Hence, proper lines of rapid strategies are there to flatten the curve.","PeriodicalId":53378,"journal":{"name":"Berkala Kedokteran","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47835508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Parents' Challenges to Health Services for Children with Disabilities during the COVID-19 Pandemic: Literature Review","authors":"N. Fadilah, Kadek Ayu Erika, Nur Azizah","doi":"10.20527/jbk.v19i1.15776","DOIUrl":"https://doi.org/10.20527/jbk.v19i1.15776","url":null,"abstract":"Health services are important in supporting one's health. Due to social restrictions and regulations during a pandemic, access to health services also adapts to existing conditions. The purpose of this literature study is to identify challenges for parents in providing health services for children with disabilities during the COVID-19 pandemic. This research method used a literature review design using the PubMed, ProQuest, and ScienceDirect electronic databases. It was found that 7 articles were reviewed after fulfilling the inclusion criteria and were analysed. The results of the review found that a number of reasons for children with disabilities not participating in health services were due to several factors such as social restrictions, parents, and technology. Various relevant barriers to accessing health services are identified in this literature review. Challenges to social distancing were the most frequently reported in studies. The description in this study can be used as input in considering access and form of health services for children with disabilities.","PeriodicalId":53378,"journal":{"name":"Berkala Kedokteran","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46326514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification Risk Factors of Stroke: Literature Review","authors":"Agianto Agianto, Bellia Yulise, Rismia Agustina, Ichsan Rizany","doi":"10.20527/jbk.v18i2.14516","DOIUrl":"https://doi.org/10.20527/jbk.v18i2.14516","url":null,"abstract":"State spending to deal with stroke is quite large every year due to the high incidence of stroke. Data from the Ministry of Health of Indonesia mentions an increasing number in health financing every year, the country financed stroke services by 2.56 trillion in 2018. The magnitude of the incidence of stroke can be lowered by controlling the risk factors of stroke. This study aimed to identify stroke risk factors, both those modifiable risk factors and irreversible risk factors. Research is conducted with a literature review using descriptive study methods or correlation analysis with cross sectional approaches. Sixteen articles identified from Google scholars and Garuda databases in the 2015-2020 range, and were analysed using JBI critical appraisal tools-checklist for analytical cross-sectional studies and JBI critical appraisal tools-checklist for prevalence studies. Synthesis narrative was used to analyse the articles. Out of 16 articles examined the modifiable risk factors were hypertension, dyslipidemia, diabetes mellitus, lack of physical activity, blood sugar levels, cholesterol levels in blood, alcohol and drug consumption, kidney disease, tuberculosis, heart disease, heart failure, obesity, central obesity, atrial fibrillation and also smoking are declared associated with the incidence of stroke. The irreversible risk factors are age, gender, education, family history, income employment, previous stroke history, and rural and urban distribution. Risk factors divided into two categories modifiable risk factors and irreversible risk factors. It is important to know the risk factors of stroke in order prevent the occurrence of stroke as early as possible by avoiding modifiable stroke risk factors. Hypertension, smoking, diabetes mellitus obesity, dyslipidemia, and lack of activity are the most modifiable stroke risk factors identified in the articles. The most irreversible risk factors identified in the article are age, gender, and level of education","PeriodicalId":53378,"journal":{"name":"Berkala Kedokteran","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41698285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correlation of Weaning Food with the Event of Stunting to Toddlers in Kali Rungkut Village Surabaya","authors":"Warini Ayu Safitri, N. K. Indahsari","doi":"10.20527/jbk.v18i2.14496","DOIUrl":"https://doi.org/10.20527/jbk.v18i2.14496","url":null,"abstract":"Stunting is a chronic nutritional problem due to several factors and is intergenerational. For the number of children with stunting conditions, Indonesia is ranked fifth globally. This is due to one of the reasons for the provision of less nutritious food during the growth period obtained from weaning food. The purpose of this study was to determine whether there was a relationship between the provision of weaning food and the incidence of stunting in children in Kali Rungkut Village, Surabaya.This study is a case control method of chi square analysis and kappa correlation using primary data, namely questionnaires. The number of samples that meet the criteria are 53 people, then the data is processed with SPSS.Based on the results of the study, the relationship between complementary feeding and the incidence of stunting in Toddlers in Kali Rungkut Village, Surabaya, with a p-value of 0.002 less than (0.05) and an OR of 6.429. Based on the results of the study, it can be concluded that there is a significant relationship between the provision of complementary foods and the incidence of stunting in children under five in Kalirungkut sub-district, Surabaya and the relationship between the provision of complementary foods and the incidence of stunting is a large category, which has a risk of 6,429 times greater in the provision of complementary foods, less good in the incidence of stunting than the provision of complementary foods. the good one.","PeriodicalId":53378,"journal":{"name":"Berkala Kedokteran","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44279571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Yanuar Ichrom Nahzi, Nida Amalia, Ferdy Rijaldi
{"title":"Case Description of Oral Cavity Hard Tissue Disease in Banjarmasin 2017-2020","authors":"Muhammad Yanuar Ichrom Nahzi, Nida Amalia, Ferdy Rijaldi","doi":"10.20527/jbk.v18i2.14474","DOIUrl":"https://doi.org/10.20527/jbk.v18i2.14474","url":null,"abstract":"Disease that rank first from the list of ten major diseases that are often complained by Indonesian people is oral cavity diseases. The Hard Tissue of oral cavity lesions can be in the form od caries, periodontal disease, periapical abnormalities, cysts, and tumor in the oral cavity. Based on the Riset Kesehatan Dasar (Riskesdas) in 2018 the prevalence of dental and oral problem in Indonesia in South Kalimantan was 59,6%. The purpose of this study is to determine the description of oral hard tissue lesions in Banjarmasin city in 2017-2020. This research is descriptive study using a cross sectional approach. The variables on this study were oral hard tissue disease and gender, the data used were secondary data that obtained from the recording and reporting of the LB-1 form at Banjarmasin Community Health Center. The results showed that the most common type of disease on oral hard tissue case in 2017-2020 was pulp and periapical tissue disease, in 2017 it was 12,640 cases (78.37%), in 2018 it was 18,184 cases (51,45%). In 2019 it was 22,493 cases (57.07%) and in 2020 it was 3,220 cases (58.59%). In 2017, the most cases were in men with total of 7,887 cases (62.50%),while in 2018, 2019, and 2020, the most cases were in women with a total of 11,621 cases (64,29%), 14,285 cases (63.51%) and 1,892 cases (58.76%). It is necessary to prevent oral hard tissue disease, especially in pulp and periapical tissue disease.","PeriodicalId":53378,"journal":{"name":"Berkala Kedokteran","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44089536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of Inflammation Parameters in the Elderly and Adults to the Outcomes of Confirmed Covid-19 Patients","authors":"P. Putra, A. Assagaf, M. Isa","doi":"10.20527/jbk.v18i2.14481","DOIUrl":"https://doi.org/10.20527/jbk.v18i2.14481","url":null,"abstract":"The elderly is susceptible to infection due to immunosenescence causing high cases of infection with significant deaths due to Coronavirus Disease 2019 (COVID-19) pandemic. Many studies have examined inflammatory parameters as predictors of the outcome of COVID-19 cases, but there has been no comparison of the values of these parameters between the elderly and adult groups. This study aims to explain the relationship between inflammatory parameter values in the elderly group compared to the adult group with the outcome of confirmed COVID-19 patients treated at Ulin Hospital Banjarmasin. An analytical observational cross-sectional design study with the samples was patients with examined Neutrophil-Lymphocyte Ratio (NLR), Platelet-Lymphocyte Ratio (PLR), Erythrocyte Sedimentation Rate (ESR), and C-reactive protein (CRP) when admitted to the COVID-19 isolation room at Ulin Banjarmasin Hospital and was divided into elderly and adult groups. A bivariate correlation test was conducted to assess the relationship between each parameter and continued by finding the cut-off value using the Receiver Operating Characteristic (ROC) curve. Odds Ratio (OR) is sought to find out how much the outcome risk prediction is based on the cut-off value. The relative coefficients of NLR, PLR, CRP, and ESR in the elderly group of 1147 subjects were 0.304; 0.119; 0.378; and 0.071. While the cut-off value of NLR, PLR, CRP, and ESR for the elderly group is 6.77; 257.5; 95.65, and 53.4. Outcomes prediction of the confirmed COVID-19 patients in the elderly group from the most significant are CRP, NLR, and PLR. Meanwhile, ESRs are statistically insignificant. The results showed that there are differences in inflammatory parameter values between the elderly and adult groups to predict the outcome of COVID-19 cases.","PeriodicalId":53378,"journal":{"name":"Berkala Kedokteran","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43842084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}