Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al最新文献_第5页

Nuclear pores in luteal cells during pregnancy and after parturition and pup removal in the rat. A freeze-fracture study. 大鼠妊娠和分娩后黄体细胞核孔的变化。一项冻裂研究。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2010-08-01 DOI: 10.32604/BIOCELL.2010.34.081

J. Cavicchia, Gustavo Guembe, M. Foscolo

{"title":"Nuclear pores in luteal cells during pregnancy and after parturition and pup removal in the rat. A freeze-fracture study.","authors":"J. Cavicchia, Gustavo Guembe, M. Foscolo","doi":"10.32604/BIOCELL.2010.34.081","DOIUrl":"https://doi.org/10.32604/BIOCELL.2010.34.081","url":null,"abstract":"In a previous paper we described a pronounced increase of apoptotic nuclei in rat corpus luteum of pregnancy whose programmed chromatin degeneration was induced by the progesterone antagonist mifepristone. Those observations encouraged us to study the apoptotic nuclear membrane during pregnancy and after parturition and pup removal, by using a freeze-fracture technique which allows us to observe 'en face' the nuclear envelop and also permits nuclear pore counting. This study was complemented with the TUNEL assay (TdT-mediated dUTP nick-end labelling). Changes in nuclear pores during pregnancy begin with an intense reduction in number but still showing an even distribution on the nuclear membrane, never forming aggregations sharply separated from pore-free areas, which are characteristic of other apoptotic models. Electron microscopy of thin-sections shows, coincidently with findings in the freeze-fracture replicas, a moderately irregular aggregation of marginal heterochromatin condensations. After nuclear fragmentation and micronuclear formation, pores behave in the usual manner in other apoptotic models, i.e., mainly showing migrations of nuclear pores toward the chromatin-free areas. The present results support the hypothesis that nuclear pore complexes are dynamic structures, which permit their migration toward nuclear membrane areas devoid of chromatin aggregations that might block the nucleocytoplasmic transport in such areas.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127960680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In vitro effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line. 2-甲氧基雌二醇-双磺胺酸对MCF-7乳腺腺癌细胞生长、形态和细胞周期动力学的体外影响

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2010-08-01 DOI: 10.32604/BIOCELL.2010.34.071

C. Vorster, A. Joubert

引用次数: 14

CD44 is involved in CXCL-12 induced acute myeloid leukemia HL-60 cell polarity. CD44参与CXCL-12诱导的急性髓性白血病HL-60细胞极性。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2010-08-01 DOI: 10.32604/BIOCELL.2010.34.091

Li-ping Zhou, Xiaolin Guo, B. Jing, Lianshuang Zhao

引用次数: 14

NFAT regulates CSF-1 gene transcription triggered by L-selectin crosslinking. NFAT调控l -选择素交联引发的CSF-1基因转录。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2010-08-01 DOI: 10.32604/BIOCELL.2010.34.057

Cuixia Chen, Lingling Cui, Xin Shang, Xianlu Zeng

引用次数: 5

Secondary zoospores in the algal endoparasite Maullinia ectocarpii (Plasmodiophoromycota). 藻内寄生虫外carpimaullinia (Plasmodiophoromycota)的次生游动孢子。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2010-04-01 DOI: 10.32604/BIOCELL.2010.34.045

E. Parodi, E. Cáceres, R. Westermeier, D. Müller

引用次数: 9

Cobalt chloride stimulates phosphoinositide 3-kinase/Akt signaling through the epidermal growth factor receptor in oral squamous cell carcinoma. 在口腔鳞状细胞癌中，氯化钴通过表皮生长因子受体刺激磷酸肌肽3-激酶/Akt信号通路。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2010-04-01 DOI: 10.32604/BIOCELL.2010.34.015

M. Ryu, Jeongsook Park, Ji Eun Park, Jin Chung, C. Lee, H. Park

{"title":"Cobalt chloride stimulates phosphoinositide 3-kinase/Akt signaling through the epidermal growth factor receptor in oral squamous cell carcinoma.","authors":"M. Ryu, Jeongsook Park, Ji Eun Park, Jin Chung, C. Lee, H. Park","doi":"10.32604/BIOCELL.2010.34.015","DOIUrl":"https://doi.org/10.32604/BIOCELL.2010.34.015","url":null,"abstract":"Tumor cells are often found under hypoxic conditions due to the rapid outgrowth of their vascular supply, and, in order to survive hypoxia, these cells induce numerous signaling factors. Akt is an important kinase in cell survival, and its activity is regulated by the upstream phosphoinositide 3-kinase (PI3K) and receptor tyrosine kinases (RTKs). In this study, we examined Akt activation and RTKs/PI3K/Akt signaling using the hypoxia-mimetic cobalt chloride in oral squamous carcinoma cells. Cobalt chloride increases Akt phosphorylation in both a dose- and time-dependent manner. Blocking the activation of the PI3K/Akt pathway using LY294002 abolished Akt activation in response to cobalt chloride, suggesting that Akt phosphorylation by cobalt chloride is dependent on PI3K. In addition, activation of the PI3K/Akt pathway seems to rely on the epidermal growth factor receptor (EGFR), since the inhibition of EGFR attenuated cobalt chloride-induced Akt activation. The results in this study also demonstrate that cobalt chloride increases EGFR protein levels and induces oral squamous cell carcinoma cells to enter S phase.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"11 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120851739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Taxus globosa S. cell lines: initiation, selection and characterization in terms of growth, and of baccatin III and paclitaxel production. 红豆杉(Taxus globsa)细胞系的起始、选择和生长特性，以及baccatin III和紫杉醇的生产。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2010-04-01 DOI: 10.32604/BIOCELL.2010.34.001

D. M. Barradas-Dermitz, P. M. Hayward-Jones, M. Mata-Rosas, B. Palmeros-Sánchez, Oscar Platas-Barradas, Rodolfo F Velásquez-Toledo

{"title":"Taxus globosa S. cell lines: initiation, selection and characterization in terms of growth, and of baccatin III and paclitaxel production.","authors":"D. M. Barradas-Dermitz, P. M. Hayward-Jones, M. Mata-Rosas, B. Palmeros-Sánchez, Oscar Platas-Barradas, Rodolfo F Velásquez-Toledo","doi":"10.32604/BIOCELL.2010.34.001","DOIUrl":"https://doi.org/10.32604/BIOCELL.2010.34.001","url":null,"abstract":"Of the initial six cell lines originating from explants of Taxus globosa, or Mexican yew (stem internode, leaves and meristematic tissue), three were selected for their microbial and oxidation resistance, two from leaves and the other from stem internode. A study of their behavior, both in terms of cell growth, and of baccatin III and paclitaxel production, was developed in suspension cultures with an initially standardized biomass (fresh weight 0.23 g/L) using modified Gamborg's B5 medium, and an elicitor (methyl jasmonate), on either the first or seventh day of culture, at several levels (0, 0.1, 1, 10, 100 microM). In most of the conditions used, the three cell lines showed growth associated baccatin III production. The cell line from stem internode was the highest producer of baccatin III using 1 microM elicitor, sampling at 10 days (p < or = 0.01, 6.45 mg/L). This same line also had the highest biomass production (6.85 g/L, p < or = 0.01) at 10 days of culture but at the higher elicitor concentration of 10 microM. All three cell lines did not produce paclitaxel under experimental conditions used.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125660039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Leaf surfaces of Gomphrena spp. (Amaranthaceae) from Cerrado biome. 塞拉多生物群系中苋属植物的叶表面。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2010-04-01 DOI: 10.32604/BIOCELL.2010.34.023

S. M. Fank-de-Carvalho, Misléia Rodrigues de Aguiar Gomes, Pedro Ítalo Tanno Silva, S. Báo

{"title":"Leaf surfaces of Gomphrena spp. (Amaranthaceae) from Cerrado biome.","authors":"S. M. Fank-de-Carvalho, Misléia Rodrigues de Aguiar Gomes, Pedro Ítalo Tanno Silva, S. Báo","doi":"10.32604/BIOCELL.2010.34.023","DOIUrl":"https://doi.org/10.32604/BIOCELL.2010.34.023","url":null,"abstract":"The leaf structure and micromorphology characterize plant species and reflex its interactions with the environment. Leaf epidermis sculptures aid high transpiration plants on light reflection. The form and distribution of epicuticular wax crystalloids are important to characterize the surface. Aiming to know the micromorphology and the ultrastructure of G. arborescens, G. pohlii and G. virgata, leaves of these Cerrado native species were collected in Brasília, Distrito Federal, Brazil, at the Olympic Center of the Universidade de Brasília and at Reserva Ecológica do Roncador. Leaves of G. globosa, an Indian native species, were also studied for comparison. Leaves were fractionated, fixed and treated for observation under optical and scanning electron microscope. A description of the leaf epidermis is provided, along with some quantitative data to help the species taxonomy and support future studies on their physiology: all species are amphistomatic and have Stomatal Index between 7.27 and 18.99. The Gomphrena spp. studied have epicuticular wax platelets and wax sculptures over their larger trichome, which are relevant for their taxonomy. Over the Cerrado species cuticle, epicuticular wax is damaged by fungi hyphae development. The presence of epicuticular wax on Gomphrena spp. leaves corroborates the phylogenetical alliance between Amaranthaceae and Chenopodiaceae.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131837795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Effect of morphological heterogeneity of somatic embryos of Melia azedarach on conversion into plants. 苦楝体细胞胚形态异质性对植株转化的影响。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2010-04-01 DOI: 10.32604/BIOCELL.2010.34.007

S. Vilà, Ana Maria Gonzalez, H. Rey, L. Mroginski

{"title":"Effect of morphological heterogeneity of somatic embryos of Melia azedarach on conversion into plants.","authors":"S. Vilà, Ana Maria Gonzalez, H. Rey, L. Mroginski","doi":"10.32604/BIOCELL.2010.34.007","DOIUrl":"https://doi.org/10.32604/BIOCELL.2010.34.007","url":null,"abstract":"Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 microM thidiazuron or 0.45 microM dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized in four morphological classes based on the presence of single or fused embryos and if they remained united or not to the original explant; that were evaluated histologically. The somatic embryos of every category were transferred, in groups or individually, on a 1/4 MS medium. Bipolar embryos, the more typically normal ones, had well defined shoot and root apical meristems and produced single plants; subcultured individually their conversion was 28%, and subcultured in groups the conversion declined to 6.8%. Fused embryos subcultured in groups had only a 2.1% conversion and produced plants with fused stems. None conversion rate in the others classes was associated to poorly developed shoot and root meristematic areas or with their absence. The converted plants were acclimatized and transferred, in a mist, to soil, with an independent of the class 95% survival rate.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115286380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Interleukin-1 beta regulates metalloproteinase activity and leptin secretion in a cytotrophoblast model. 白细胞介素-1 β在细胞滋养细胞模型中调节金属蛋白酶活性和瘦素分泌。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2010-04-01 DOI: 10.32604/BIOCELL.2010.34.037

V. Fontana, M. Sanchez, E. Cebral, J. Calvo

引用次数: 26