Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005.最新文献

Mechanical and biochemical stimuli alter endothelial cell gene expression 机械和生化刺激改变内皮细胞基因表达

Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005. Pub Date : 2005-04-02 DOI: 10.1109/NEBC.2005.1431996

J. McCann, S. Peterson, M. Plesniak, T. Webster, K. M. Haberstroh

{"title":"Mechanical and biochemical stimuli alter endothelial cell gene expression","authors":"J. McCann, S. Peterson, M. Plesniak, T. Webster, K. M. Haberstroh","doi":"10.1109/NEBC.2005.1431996","DOIUrl":"https://doi.org/10.1109/NEBC.2005.1431996","url":null,"abstract":"Cardiovascular diseases result in altered endothelial cell functions because of changes in blood flow properties. To understand the relationship between cell environment and behavior, endothelial cell expression of key genes (COX-2, ecNOS, and PDGF-B) was analyzed following exposure to defined mechanical and chemical conditions. Cells were exposed to control medium, control medium and physiological flow, control medium and physiological pressures, control medium and pathological pressures, flow-conditioned medium, flow-conditioned medium and physiological flow, or flow-conditioned medium and physiological pressures. Endothelial cells were sensitive to both their chemical and mechanical environments; for example, while flow, pressure, and flow-conditioned medium each individually affected gene expression, this expression was most dramatically altered when cells were simultaneously exposed to mechanical and chemical stimuli. Interestingly, gene expression following pressure exposure was always less than that following flow. Next, this study began to investigate endothelial cell behavior under pathological flows using a stenotic glass tube with dimensions similar to the carotid artery. Particle image velocimetry (PIV) measurements were utilized to characterize flow within such tubes; in the future, these flow patterns will be correlated with endothelial cell gene expression. Ultimately, these flow-cell relationships may aid in predicting sites of disease expansion downstream of a stenosis.","PeriodicalId":256365,"journal":{"name":"Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005.","volume":"119 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115454899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular engineering approaches for quantifying hepatic gene activity in embryonic stem cells 定量测定胚胎干细胞肝脏基因活性的分子工程方法

Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005. Pub Date : 2005-04-02 DOI: 10.1109/NEBC.2005.1431995

E. J. Wallenstein, R. Schloss, M. Yarmush

{"title":"Molecular engineering approaches for quantifying hepatic gene activity in embryonic stem cells","authors":"E. J. Wallenstein, R. Schloss, M. Yarmush","doi":"10.1109/NEBC.2005.1431995","DOIUrl":"https://doi.org/10.1109/NEBC.2005.1431995","url":null,"abstract":"Although defined differentiation strategies exist for generating hepatocyte-like cells from mouse embryonic stem (ES) cells, these systems yield a heterogeneous end population, from which it is difficult to assay, purify and quantify cells of interest and understand their gene expression profile. Using non-viral vector plasmids, we have designed several constructs for exploring the real-time expression levels of alpha feto-protein and albumin. These vectors encode for enhanced green fluorescent protein (EGFP) and are driven by the regulatory elements of the gene of interest Via transient transfection, the DNA reporter plasmids are delivered into the ES cells to monitor gene behavior and the portion of cells that are liver-like. Parallel transfections using constitutive reporters provide a normalization control and yield insight into changes in gene uptake with varied differentiation. Gene behavior is quantified based on flow cy to me try and cells of interest can be further purified from the remaining population via a cell sorter. This process enables additional enzymatic assays to be performed on this homogenous group. In addition to providing a vivid tool for understanding specific gene regulatory motifs, the reporters have further application in assaying cell-cell interactions, cell communication and maturation of cellular components.","PeriodicalId":256365,"journal":{"name":"Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005.","volume":"131 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115738538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Devices used to expose the posterior coronary artery in OPCABG surgery may cause ischemia OPCABG手术中用于暴露冠状动脉后段的装置可能导致缺血

Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005. Pub Date : 2005-04-02 DOI: 10.1109/NEBC.2005.1431967

R. Dzwonczyk, C. D. del Rio, B. Sun, R. Michler, M. Howie

{"title":"Devices used to expose the posterior coronary artery in OPCABG surgery may cause ischemia","authors":"R. Dzwonczyk, C. D. del Rio, B. Sun, R. Michler, M. Howie","doi":"10.1109/NEBC.2005.1431967","DOIUrl":"https://doi.org/10.1109/NEBC.2005.1431967","url":null,"abstract":"Off-pump coronary artery bypass graft (OPCABG) surgery is commonly used to revascularize diseased hearts. In order to graft a posterior artery in this procedure, the heart must be lifted from the chest cavity using an apical suction device to expose the surgical field. In the process, this device may cause unwanted myocardial ischemia. We measured myocardial electrical impedance (MEI), a parameter that responses to myocardial ischemia, as well as ST-segment changes during OPCABG surgery in five patients undergoing revascularization of the left anterior descending (LAD) and posterior descending coronary artery (PDA). MEI increased with the application of the suction device in these patients, suggesting myocardial ischemia. ST-segment changes were negligible while the heart was raised from the chest cavity but increased immediately when the heart was returned to the normal position. Our data suggest that the apical suction device causes ischemia that is virtually undetected with ST-segment monitoring while the heart is raised and electrically disconnected from the body. MEI has the potential to detect intra-operative ischemia monitor.","PeriodicalId":256365,"journal":{"name":"Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005.","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121051378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

3-D computational fluid dynamics (CFD) modeling for the transport of chemotherapeutic drugs in the human brain 化疗药物在人脑中的传输的三维计算流体动力学(CFD)建模

Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005. Pub Date : 2005-04-02 DOI: 10.1109/NEBC.2005.1432025

A. Kumar

{"title":"3-D computational fluid dynamics (CFD) modeling for the transport of chemotherapeutic drugs in the human brain","authors":"A. Kumar","doi":"10.1109/NEBC.2005.1432025","DOIUrl":"https://doi.org/10.1109/NEBC.2005.1432025","url":null,"abstract":"A three dimensional computational fluid dynamics (CFD) model of the Fall Cerebri and a composite cylinder representing gray matter and white matter of the human brain is developed to predict the transport of interstitial infusion of chemotherapeutic drugs. Brain tissues are treated as porous media and characterized by the porosity and the resistance coefficient. White matter, which is anisotropic in nature due to the presence of axon fibers, has different properties in longitudinal and transverse directions. Anisotropy has been defined as the ratio of resistance coefficients in longitudinal and transverse directions. The transport of the drug in white and gray matter is governed by convection and/or diffusion. Temporal and spatial mass concentration of the drug is determined in each case. It was observed that bulk flow or convection enhanced delivery (CED) was more effective for the increase of mass concentration and penetration of the drug molecules into the brain. Also, in white matter penetration of the drug molecules in the fiber direction was greater than penetration in the transverse direction. Obtaining an analytical solution will be incorporated in the next phase of the research.","PeriodicalId":256365,"journal":{"name":"Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005.","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127339891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Deterministic Boolean networks (DBN) modeling of molecular biology: DNA replication 分子生物学的确定性布尔网络(DBN)建模:DNA复制

Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005. Pub Date : 2005-04-02 DOI: 10.1109/NEBC.2005.1431954

J. W. Yeol, W. Samarrai, I. Barjis, Y. S. Ryu

引用次数: 0

A humidifier for olfaction studies during functional magnetic resonance imaging 功能磁共振成像中用于嗅觉研究的加湿器

Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005. Pub Date : 2005-04-02 DOI: 10.1109/NEBC.2005.1432028

M. Corcoran, H. Zee, C. Sotak, Y. Mendelson

{"title":"A humidifier for olfaction studies during functional magnetic resonance imaging","authors":"M. Corcoran, H. Zee, C. Sotak, Y. Mendelson","doi":"10.1109/NEBC.2005.1432028","DOIUrl":"https://doi.org/10.1109/NEBC.2005.1432028","url":null,"abstract":"Functional magnetic resonance imaging has been used to study the transmission of olfactory sensations in the nervous system. Researchers hypothesize that olfactory sensation depends on the humidity of the air containing the odorant. We were asked to create this device for a biomedical design course offered at Worcester Polytechnic Institute. Our purpose was to create a device for researchers who are conducting initial research on rat models. The device allows researchers to provide a humidified source of oxygen safely to an anesthetized laboratory rat for up to six hour experimental sessions. Furthermore, the humidifier allows the user to manually control the humidity by a simple valve. In addition, the humidifier increases and decreases the humidity by slow increments to ensure the safety of the lab animal as well as the accuracy of the humidity readings. Finally, the design consists of two ports where a gaseous or liquid form odorant may be administered and evaluated for olfactory fMRI information. This device is a user-friendly, user-safe, and animal-safe method of odorant administration for fMRI studies. Moreover, this device can be easily expanded to become a computer-controlled humidifying system.","PeriodicalId":256365,"journal":{"name":"Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005.","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126189064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From mollusk to Matlab: experiential learning for the undergraduate biomedical engineer 从软体动物到Matlab:生物医学工程本科体验式学习

Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005. Pub Date : 2005-04-02 DOI: 10.1109/NEBC.2005.1431945

A. Wagner, R. Bartels, J. DiCecco, Y. Sun

引用次数: 0

Design and freeform fabrication of load bearing tissue scaffolds 承载组织支架的设计和自由成形

Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005. Pub Date : 2005-04-02 DOI: 10.1109/NEBC.2005.1431974

B. Starly, L. Shor, Z. Fang, W. Sun

引用次数: 2

An approach to microCT image processing 一种微ct图像处理方法

Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005. Pub Date : 2005-04-02 DOI: 10.1109/NEBC.2005.1431961

M. Clark, J. DaPonte, T. Sadowski

引用次数: 0

Development of an automated medical equipment replacement planning system in hospitals 医院医疗设备自动化更换计划系统的开发

Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005. Pub Date : 2005-04-02 DOI: 10.1109/NEBC.2005.1431922

D. Rajasekaran

{"title":"Development of an automated medical equipment replacement planning system in hospitals","authors":"D. Rajasekaran","doi":"10.1109/NEBC.2005.1431922","DOIUrl":"https://doi.org/10.1109/NEBC.2005.1431922","url":null,"abstract":"Replacement requests for healthcare technology and equipment in hospitals are often managed without the benefit of factual, safety, technical, financial and performance data. An automated equipment replacement planning system (ERPS) has been developed to identify equipment most in need of replacement in order to optimize the utilization of capital budget resources, the attention to patient safety and efficiency of the healthcare process. Rules have been developed to assist in determining which equipment should be prioritized for replacement. The ERPS consists of a skeleton database in which the replacement rules have been programmed. Data from a Clinical Engineering department's equipment management database are imported into the skeleton database of the ERPS. The imported data are evaluated by a program from the replacement-rule base to produce a relative replacement number (RRN) for each medical device in the inventory of the hospital. This number enables prioritization of all medical devices identifying the recommended order of replacement. The results are compared with replacement priorities obtained from traditional manual methods. This ERPS is designed such that replacement criteria can be custom edited for each hospital that will use the system, according to its own requirements and restrictions.","PeriodicalId":256365,"journal":{"name":"Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005.","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123909586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22