Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics最新文献

Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics Pub Date : 2019-05-22 DOI: 10.1117/12.2523431

引用次数: 2

Quantificiation of hemoglobin concentrations in whole blood by visible-light spectroscopic optical coherence tomography (Conference Presentation) 用可见光光谱光学相干断层扫描定量测定全血血红蛋白浓度(会议报告)

Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics Pub Date : 2019-03-13 DOI: 10.1117/12.2509866

C. Veenstra, S. Kruitwagen, Dafne Groener, Wilma Petersen, W. Steenbergen, Nienke Bosschaart

{"title":"Quantificiation of hemoglobin concentrations in whole blood by visible-light spectroscopic optical coherence tomography (Conference Presentation)","authors":"C. Veenstra, S. Kruitwagen, Dafne Groener, Wilma Petersen, W. Steenbergen, Nienke Bosschaart","doi":"10.1117/12.2509866","DOIUrl":"https://doi.org/10.1117/12.2509866","url":null,"abstract":"A decreased hemoglobin concentration (tHb) in blood (anemia) is associated with impaired oxygen delivery to organs, which can result in organ damage and heart failure. Currently, tHb analysis requires invasive methods (e.g. a fingerstick), which are time consuming and cause discomfort to the patient. Using optical spectroscopy, the tHb can be estimated by quantifying light absorption in blood. However, the accuracy of current noninvasive optical techniques for tHb quantification is limited by the background attenuation of skin and the unknown blood volume fraction in the total optical probing volume.\u0000\u0000Spectroscopic optical coherence tomography (sOCT) allows for quantitative measurements of the optical attenuation in a confined measurement volume, potentially enabling non-invasive estimation of the hemoglobin concentration within individual blood vessels. Although multiple studies have shown that sOCT is capable of quantifying localized oxygen saturation, quantification of the tHb has not yet been reported for physiologically relevant concentrations.\u0000\u0000With a home-built visible-light sOCT system we quantified optical attenuation in the visible wavelength range (450–600nm). Implementation of both zero-delay acquisition and focus tracking optimized system sensitivity and ensured that the measured attenuation is only affected by the attenuation of the sample itself. \u0000\u0000We validated our method ex-vivo on human whole blood from healthy volunteers (tHb within 12-18 g/dL). The hematocrit was varied to cover the entire pathophysiological range (tHb within 9-21 g/dL) by either dilution with PBS, or plasma removal. Our system quantified the tHb in whole blood throughout the entire pathophysiological range with an accuracy of 10%.","PeriodicalId":252939,"journal":{"name":"Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121689934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring therapeutic response of murine tumor allografts of colon carcinoma in response to combined immunotherapy and chemotherapy (Conference Presentation) 小鼠结肠癌同种异体肿瘤移植对免疫化疗联合治疗反应的监测(会议报告)

Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics Pub Date : 2019-03-13 DOI: 10.1117/12.2510194

G. Greening, S. Bess, Haley M. James, Ariel I. Mundo, Timothy J. Muldoon

{"title":"Monitoring therapeutic response of murine tumor allografts of colon carcinoma in response to combined immunotherapy and chemotherapy (Conference Presentation)","authors":"G. Greening, S. Bess, Haley M. James, Ariel I. Mundo, Timothy J. Muldoon","doi":"10.1117/12.2510194","DOIUrl":"https://doi.org/10.1117/12.2510194","url":null,"abstract":"Immunotherapy, an emerging field in cancer therapeutics, in colon cancer aims to reduce pre-surgical tumor burden by regulating host immune checkpoints, and when used in combination with neoadjuvant chemotherapy, may improve tumor therapeutic response. One such immune checkpoint is CCL2 (monocyte chemoattractant protein-1)-mediated recruitment of monocytes, which differentiate into tumor-associated macrophages (TAMs) in the tumor microenvironment that promote angiogenesis and tumorigenesis. Thus, CCL2 blockade may play an anti-tumor role via effects on tumor perfusion. However, there have been no studies investigating CCL2 blockade immunotherapy combined with chemotherapy in an animal model of colon cancer. Furthermore, there is a need to longitudinally assess tumor therapeutic response throughout treatment. In this study, CT26 murine colon carcinoma was injected into the flanks of Balb/c mice (n=80) to form tumor allografts. Mice in the key experimental group received combined chemotherapy (5-flurouracil) and immunotherapy (anti-CCL2), with appropriate controls. Tumor therapeutic response was monitored using diffuse reflectance spectroscopy (DRS) by measuring the tumor perfusion metrics, hemoglobin concentration and oxygenation. End-point immunohistochemical analysis was used to quantify TAM fraction (CD68 and DAPI), TAM polarization (iNOS and CD206), and hypoxia (pimonidazole) to spatially and temporally correlate to DRS results. The central hypothesis was that decreasing TAMs via CCL2 blockade alters tumor perfusion, thereby increasing tumor response to 5-fluorouracil. This study may potentially demonstrate an effective immunotherapy approach (CCL2 blockade) and a viable method to longitudinally and non-invasively assess tumor therapeutic response to such immunotherapy (DRS) in mouse allograft models of colon cancer.","PeriodicalId":252939,"journal":{"name":"Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127524342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue auto-fluorescence lifetime imaging in porcine burn wound models (Conference Presentation) 组织自荧光寿命成像在猪烧伤模型中的应用(会议报告)

Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics Pub Date : 2019-03-13 DOI: 10.1117/12.2510590

Suhaib Hashem, J. Lo

引用次数: 0

Objective determination of heart failure grade using short wave infrared (SWIR) molecular chemical imaging (Conference Presentation) 利用短波红外(SWIR)分子化学成像客观确定心力衰竭分级(会议报告)

Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics Pub Date : 2019-03-13 DOI: 10.1117/12.2509802

Aaron Smith, Shona Stewart, A. Samiei, A. Bangalore, Heather Gomer, C. Post, P. Treado, Jeffrey A. Cohen

{"title":"Objective determination of heart failure grade using short wave infrared (SWIR) molecular chemical imaging (Conference Presentation)","authors":"Aaron Smith, Shona Stewart, A. Samiei, A. Bangalore, Heather Gomer, C. Post, P. Treado, Jeffrey A. Cohen","doi":"10.1117/12.2509802","DOIUrl":"https://doi.org/10.1117/12.2509802","url":null,"abstract":"Heart failure (HF) occurs when the heart is unable to pump enough blood to meet blood and oxygen requirements and is among the most common causes for hospitalization in the United States. A retrospective analysis determined that 22% of HF patients are readmitted within 30 days of release from the hospital, and the costs for readmission are substantial. Measuring the severity of peripheral edema is one method for monitoring the treatment of a HF patient. Pitting peripheral edema is a subjective measure administered by clinicians who create an indentation mid-tibia and observe depth and time to resolve the indentation. The results are graded 0, 1, 2, 3 or 4, and this information is used in the patient treatment plan. ChemImage is engaged in a clinical study to determine whether Molecular Chemical Imaging (MCI) in the short wave infrared (SWIR) spectral region can provide an objective measure of peripheral edema in HF patients. In this paper, the performance of SWIR MCI for discriminating between healthy volunteers and HF patients with high grade pitting edema will be presented. This technology may provide a non-invasive methodology for quantitative peripheral edema measurement. As the technology matures, it is envisioned patient self-monitoring, with wireless transmission of edema levels while at home, can aid clinicians in monitoring HF patients for necessary treatment changes remotely, to improve patient outcomes, and ultimately, reduce HF hospital readmission rates.","PeriodicalId":252939,"journal":{"name":"Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126668160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Coke or Diet Coke? (Conference Presentation) 可乐还是健怡可乐?(会议)

Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics Pub Date : 2019-03-13 DOI: 10.1117/12.2510939

V. Yakovlev

引用次数: 0

Non-contact biophotonic assessment of changes in central venous pressure using photoplethysmographic imaging (Conference Presentation) 非接触生物光子评估中心静脉压的变化使用光容积脉搏图成像(会议报告)

Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics Pub Date : 2019-03-13 DOI: 10.1117/12.2510763

R. Amelard, J. Au, K. Murray, D. Greaves, R. Hughson

引用次数: 0

Changes of mean arterial pressure affect spinal cord oxygenation as monitored by an implantable near-infrared spectroscopy sensor in an animal model of acute spinal cord injury (Conference Presentation) 急性脊髓损伤动物模型植入式近红外光谱传感器监测平均动脉压变化对脊髓氧合的影响(会议报告)

Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics Pub Date : 2019-03-13 DOI: 10.1117/12.2506715

B. Shadgan, Allan Fong, N. Manouchehri, K. So, Katelyn Shortt, F. Streijger, A. Macnab, B. Kwon

{"title":"Changes of mean arterial pressure affect spinal cord oxygenation as monitored by an implantable near-infrared spectroscopy sensor in an animal model of acute spinal cord injury (Conference Presentation)","authors":"B. Shadgan, Allan Fong, N. Manouchehri, K. So, Katelyn Shortt, F. Streijger, A. Macnab, B. Kwon","doi":"10.1117/12.2506715","DOIUrl":"https://doi.org/10.1117/12.2506715","url":null,"abstract":"Introduction: Current clinical practice guidelines for acute spinal cord injury (SCI) patients suggest that increasing the mean arterial pressure (MAP) to 85-90 mmHg may improve spinal cord (SC) hemodynamics and oxygenation. The purpose of this study was to examine this effect using an implantable Near Infra-Red Spectroscopy (NIRS) sensor. Methods: Nine anesthetized Yorkshire pigs were studied. A multi-wavelength NIRS system with a custom-made miniaturized optical sensor was applied directly onto the SC dura at T9 to measure tissue oxygenation and hemodynamics within the SC non-invasively. To validate the NIRS measures, an invasive Intraparenchymal (IP) combined O2/blood flow sensor was inserted directly into the SC adjacent to the NIRS probe at T11. Using NIRS, the SC tissue oxygenation percentage (TOI%), as well as concentrations of oxygenated, deoxygenated and total hemoglobin, were monitored before, during and after episodes of MAP alterations. Using norepinephrine and nitroprusside, MAP was increased and decreased by 20mmHg for 30 min periods, simulating the types of hemodynamic changes that SCI patients experience post-injury. Results: Changes in SC hemodynamics and oxygenation levels were detected in all subjects as measured by both the invasive IP and the non-invasive NIRS sensors. Changes of TOI% during MAP increase (1.64%, p<0.005) and decrease (-3.97%, p<0.005) were significant. A consistent decrease in TOI (-15.94%, p<0.005) was observed following SCI, indicating SC tissue hypoxia at the injury site. Conclusions: Using a miniaturized SC NIRS sensor we have shown the significant effect of MAP alterations on tissue oxygenation within the injured SC.","PeriodicalId":252939,"journal":{"name":"Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122933434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical waveguides for on-chip fluorescence measurements (Conference Presentation) 片上荧光测量用光波导(会议报告)

Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics Pub Date : 2019-03-13 DOI: 10.1117/12.2508228

P. Lin, G. Coté, Kristen C. Maitland, Tiening Jin, Junchao Zhou, Paul Gordon, Cyril Soliman

{"title":"Optical waveguides for on-chip fluorescence measurements (Conference Presentation)","authors":"P. Lin, G. Coté, Kristen C. Maitland, Tiening Jin, Junchao Zhou, Paul Gordon, Cyril Soliman","doi":"10.1117/12.2508228","DOIUrl":"https://doi.org/10.1117/12.2508228","url":null,"abstract":"Optical waveguides using a visible transparent nitride were developed to perform fluorescence measurement on a chip. Through finite difference time domain (FDTD) design, the exciting green light was guided by the micron-scale ridge waveguide, while its evanescent wave was expanded outside the waveguide surface and capable to efficiently excite the fluorescent molecules that were approaching the waveguide facets. Since the waveguide was centimeters long, it has a longer fluorescence excitation path comparing to traditional samples prepared for microscopy measurements. As result, the waveguide device can excite stronger fluorescent signals. In addition, the nitride waveguide was prepared by the complementary metal–oxide–semiconductor (CMOS) process thus enabling high volume manufacturing and reducing the cost of the device fabrication. The AlN waveguide was then integrated with a microfluidic devices to experimentally demonstrate real-time fluorescence detection. Solution samples with different dye concentrations were sequentially injected into the microfluidic chamber. By recording the emission signals, we showed that the fluorescent signals were consistently amplified as the dye concentrations increased. In addition, real-time fluorescence detection with a response time less than seconds was achieved. The developed waveguide based fluorescence measurement provides a new miniaturized platform for low cost and highly accurate point-of-care application.","PeriodicalId":252939,"journal":{"name":"Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127216459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leukocyte dynamics in cutaneous acute graft-versus-host disease by noninvasive laser confocal video microscopy: a cross-sectional pilot study (Conference Presentation) 非侵入性激光共聚焦视频显微镜下皮肤急性移植物抗宿主病的白细胞动力学:一项横断面试点研究(会议报告)

Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics Pub Date : 2019-03-13 DOI: 10.1117/12.2510585

I. Saknite, M. Byrne, M. Jagasia, E. Tkaczyk

{"title":"Leukocyte dynamics in cutaneous acute graft-versus-host disease by noninvasive laser confocal video microscopy: a cross-sectional pilot study (Conference Presentation)","authors":"I. Saknite, M. Byrne, M. Jagasia, E. Tkaczyk","doi":"10.1117/12.2510585","DOIUrl":"https://doi.org/10.1117/12.2510585","url":null,"abstract":"Inflammatory tissue response is one of the first and most common manifestations of acute graft-versus-host disease (aGVHD), a potentially deadly immune-mediated disease that occurs in 30-60% of patients after stem cell transplantation. A fundamental challenge in developing effective treatment strategies for aGVHD is the lack of tools to study disease biology in real-time in post-transplant patients. The inflammatory tissue response causes increased expression of specialized endothelial proteins on vessel walls making leukocytes to roll, adhere and eventually extravasate into the tissue at a higher rate than in normal conditions. Although the importance of leukocyte-endothelial interactions to detect and track inflammation has been well shown in murine models, there are no published clinical studies in humans. In this study, we explore the feasibility to detect presence of aGVHD in post-transplant patients through the imaging of in vivo leukocyte motion. We used a clinical confocal microscope (Vivascope 1500) to acquire videos of 5 aGVHD patients and 5 controls (no aGVHD) within 50±30 days post-transplant. The microscope is capable of real-time imaging of individual cells in the postcapillary vessels at 9 frames per second. Through video analysis, we extracted five quantitative parameters: number and velocity of rolling leukocytes, number of adherent leukocytes (stationary >30 s), blood flow velocity, and number of vessels. In a limited number of subjects, we show that parameters characteristic of the dynamic motion in skin capillaries can be observed noninvasively in post-transplant patients. Further studies are needed to test the diagnostic potential of these parameters.","PeriodicalId":252939,"journal":{"name":"Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics","volume":"105 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125553023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0