Chinese Journal of Agricultural Biotechnology最新文献_第3页

Population genetic diversity of Phytophthora infestans from China as revealed by SSRs and RAPDs 中国疫霉群体遗传多样性的ssr和rapd分析

Chinese Journal of Agricultural Biotechnology Pub Date : 2009-08-01 DOI: 10.1017/S1479236209990301

Li Benjin, Lv Xin, Chen Qinghe, Lan Cheng-zhong, Zhao Jian, Qiu Rongzhou, Weng Qiyong

{"title":"Population genetic diversity of Phytophthora infestans from China as revealed by SSRs and RAPDs","authors":"Li Benjin, Lv Xin, Chen Qinghe, Lan Cheng-zhong, Zhao Jian, Qiu Rongzhou, Weng Qiyong","doi":"10.1017/S1479236209990301","DOIUrl":"https://doi.org/10.1017/S1479236209990301","url":null,"abstract":"Simple sequence repeats (SSR) and random amplification of polymorphic DNA (RAPD) molecular markers were used to assess the genetic diversity of 80 isolates of Phytophthora infestans in potato (Solanum tuberosum) from Fujian, Heilongjiang, Hebei and Inner Mongolia Provinces in China. Polymorphism was identified by 13 SSR primers and 14 RAPD primers in the isolates of P. infestans in potato. A total of 76 bands were amplified by SSRs, with the percentage of polymorphic bands (PPB) being 78.9% and the similarity coefficient ranging between 0.00 and 0.42. A total of 189 bands were amplified by RAPDs, with the percentage of polymorphic bands being 95.2% and the similarity coefficient ranging between 0.04 and 0.66. Analysis of genetic diversity showed that there exists higher genetic variation in the Fujian population in comparison to the populations of Heilongjiang, Hebei and Inner Mongolia. Nei's genetic identity analysis indicates that the genetic similarity between populations of Heilongjiang and Inner Mongolia is the highest and that between Fujian and Hebei is the lowest. A cluster analysis revealed that isolates from Fujian, in the south of China, are distantly related to those from Heilongjiang, Hebei and Inner Mongolia in the north, and the Fujian population is distributed among more groups than the other three, exhibiting a higher genetic diversity.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125572827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Development of AFLP markers linked to stem nematode resistance gene in sweet potato ( Ipomoea batatas (L.) Lam.) 甘薯茎秆线虫抗性基因AFLP标记的开发Lam)。

Chinese Journal of Agricultural Biotechnology Pub Date : 2009-08-01 DOI: 10.1017/S1479236209990192

J. Qin, L. Hua, Z. Hong, Wang Yu-ping, Liping Qiang, M. Daifu, X. Yiping, Li Qingchang

{"title":"Development of AFLP markers linked to stem nematode resistance gene in sweet potato ( Ipomoea batatas (L.) Lam.)","authors":"J. Qin, L. Hua, Z. Hong, Wang Yu-ping, Liping Qiang, M. Daifu, X. Yiping, Li Qingchang","doi":"10.1017/S1479236209990192","DOIUrl":"https://doi.org/10.1017/S1479236209990192","url":null,"abstract":"Amplified fragment length polymorphism (AFLP) markers linked to the stem nematode resistance gene were developed in sweet potato ( Ipomoea batatas (L.) Lam.). Using bulked segregant analysis (BSA), 800 AFLP primer combinations were screened in the resistant and susceptible bulked DNA from the 186 progeny of an F 1 single-cross population of Xu781 (resistant parent)×Xushu18 (susceptible parent), and 245 of these AFLP primers showed polymorphic bands between resistant and susceptible DNA. Primer combinations detecting polymorphism between the two bulks were used to screen the parents and eight individuals from each of the bulks. The results showed that E2M23 and E33M20 produced a specific band of about 500 bp and 200 bp in length, respectively, in the resistant plants but not in the susceptible plants, suggesting that the markers named E2M23 500 and E33M20 200 linked to a gene for stem nematode resistance. Amplified analysis of the 186 F 1 individuals indicated that the genetic distance between these two markers and the stem nematode resistance gene was 6.9 cM and 11.1 cM, respectively, measured with Mapmaker 3.0. These two AFLP markers were used to identify ten sweet potato varieties planted widely in China and the results were consistent with those of conventional resistance identification, indicating that the two markers can be used in molecular marker-assisted breeding for stem nematode resistance in the sweet potato.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"117 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117281374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Cloning of carnation GA 20-oxidase and the construction of a plant RNAi vector 石竹GA - 20氧化酶的克隆及植物RNAi载体的构建

Chinese Journal of Agricultural Biotechnology Pub Date : 2009-08-01 DOI: 10.1017/S1479236209990246

Jin Liang, L. Yun, Sun Zhen-yuan, L. Tianhong

引用次数: 0

Identification of resistance to corn-borer of transgenic maize inbred lines and hybrids with GFM Cry1A gene. 转GFM Cry1A基因玉米自交系及杂交种对玉米螟的抗性鉴定。

Chinese Journal of Agricultural Biotechnology Pub Date : 2009-08-01 DOI: 10.1017/S1479236209002654

Y. Ying, Lin Xiao-hui, Kong Xiang-mei, Jia Zhi-lei, Lin Chun-Jing, L. Cong-cong, Liu Na, Sun Chuan-bo, Li De-pu

{"title":"Identification of resistance to corn-borer of transgenic maize inbred lines and hybrids with GFM Cry1A gene.","authors":"Y. Ying, Lin Xiao-hui, Kong Xiang-mei, Jia Zhi-lei, Lin Chun-Jing, L. Cong-cong, Liu Na, Sun Chuan-bo, Li De-pu","doi":"10.1017/S1479236209002654","DOIUrl":"https://doi.org/10.1017/S1479236209002654","url":null,"abstract":"Six transgenic maize lines with the fully modified gene GFM Cry1A were obtained and polymerase chain reaction (PCR) analysis of their T 5 generation plants indicated that foreign genes could be stably inherited. Three hybrids (Simi25 Bt , Tongdan24 Bt and Jidan209 Bt ) were created using the transgenic lines and combined with regular maize inbred lines. The results of resistance identification of the transgenic inbred lines and hybrids showed that the difference of resistance among transgenic lines was very significant; there also existed a difference among individuals of the same line and among the three hybrids. Compared with the control inbred line (CK), the average of four transgenic inbred lines in the number of tunnels per stalk, larval tunnel length per plant and length of surviving larvae were decreased by 70%, 80% and 70%, respectively. Enzyme-linked immunosorbent assay (ELISA) testing showed that the average of Bt Cry1A protein expression level for six transgenic inbred lines was 0.16% total protein and the highest expression level was 0.19%. At maturity, compared with the control variety (CK), the larval tunnel length per plant of the three hybrids (Simi25 Bt , Tongdan24 Bt and Jidan209 Bt ) was decreased by 39.97%, 36.20% and 53.83%, respectively, which was a decrease of 43.36% on average. The investigation of agronomic characters showed that there was no significant difference between the improved hybrids and the control in plant height, ear length, row number per ear, kernel number per row and 100-kernels weight. It is thought that the GFM Cry1A gene can be applied to improve resistance to corn-borer, and maize inbred lines with the Bt gene can be directly applied to conventional maize breeding.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121494313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Medium selection in tissue culture of japonica rice cultivar Nipponbare and preparation of transgenic plants with an elicitor-coding gene from Magnaporthe grisea 粳稻品种“日本裸”组织培养的培养基选择及稻瘟病病菌激发子编码基因转基因植株的制备

Chinese Journal of Agricultural Biotechnology Pub Date : 2009-08-01 DOI: 10.1017/S1479236209002642

Ma Jianjun, Yang Xiu-fen, Zeng Hongmei, Yuan Jing-jing, Qiu De-wen

引用次数: 1

Cloning and eukaryotic expression of olfaction-related Gqα-protein gene in English grain aphid ( Sitobion avenae ) 英国粒蚜嗅觉相关基因gq α-蛋白的克隆及真核表达

Chinese Journal of Agricultural Biotechnology Pub Date : 2009-08-01 DOI: 10.1017/S1479236209990180

Fan Jia, Chen Ju-lian, Cheng Dengfa, Sun Jingrui

引用次数: 1

Cloning and tissue expression of the adiponectin gene in geese. 鹅脂联素基因的克隆及组织表达。

Chinese Journal of Agricultural Biotechnology Pub Date : 2009-08-01 DOI: 10.1017/S1479236209990234

GuoQing Xu, Dao-qing Gong, DongSheng Chu, D. Biao, Meng He

引用次数: 3

Analysis, gene cloning and expression of two α-amylases from Bacillus cereus 蜡样芽孢杆菌两种α-淀粉酶的分析、基因克隆及表达

Chinese Journal of Agricultural Biotechnology Pub Date : 2009-08-01 DOI: 10.1017/S1479236209002538

Zhang Linlin, W. Qi, Liao Rong-xi, Wang Yongjun, Mei Ru-hong

引用次数: 0

Detection of a quorum sensing signal molecule of Acidovorax avenae subsp. citrulli and its regulation of pathogenicity 一种蚁群感应信号分子的检测。瓜蒌及其对致病性的调节作用

Chinese Journal of Agricultural Biotechnology Pub Date : 2009-04-01 DOI: 10.1017/S1479236209002514

C. Tao, Qian Guoliang, Yang Xiao-li, Ma Junyi, Hu Baishi, L. Fengquan

引用次数: 11

Characterization and heterologous expression of testosterone-inducible regulator from Comamonas testosteroni in Escherichia coli. 睾酮单胞菌睾酮诱导调节因子在大肠杆菌中的鉴定及异源表达。

Chinese Journal of Agricultural Biotechnology Pub Date : 2009-04-01 DOI: 10.1017/S147923620900254X

C. Jian-qiu, A. Yu-fang, Zhou Yifei, P. Da-ren, Xiong Guang-Ming, Guo Yu-chun, Pan Fei, Lin Bing-Hui

{"title":"Characterization and heterologous expression of testosterone-inducible regulator from Comamonas testosteroni in Escherichia coli.","authors":"C. Jian-qiu, A. Yu-fang, Zhou Yifei, P. Da-ren, Xiong Guang-Ming, Guo Yu-chun, Pan Fei, Lin Bing-Hui","doi":"10.1017/S147923620900254X","DOIUrl":"https://doi.org/10.1017/S147923620900254X","url":null,"abstract":"The testosterone-inducible regulator ( teiR ) gene was cloned from Comamonas testosteroni chromosomal DNA, and introduced into plasmids pKtac2 (containing a tac promoter) and pK18 to yield plasmids pKtac2- teiR and pK teiR100 . The recombinant plasmids were transformed into competent Escherichia coli HB101 and total protein was extracted to detect the TeiR protein expression level using enzyme-linked immunosorbent assay (ELISA). E. coli transformed by pKtac2- teiR and pK teiR100 produced 6.65 and 5.93 μg/mg of TeiR protein, respectively. Recombinant plasmids were also co-transformed into competent E. coli HB101 with plasmid p6 [containing hsdA gene (3α-HSD/CR, 3α-hydroxysteroid dehydrogenase/carbonyl reductase encoding gene)] to reveal the relationship between 3α-HSD/CR and TeiR by ELISA. The amounts of TeiR protein expressed by E. coli containing pKtac2- teiR and pK teiR100 were 5.94 μg/mg and 5.33 μg/mg, respectively, and these increased up to 6.81 μg/mg and 6.10 μg/mg after inducing with 1 mmol/l isopropyl-β- d -thiogalactoside (IPTG). Interestingly, 3α-HSD/CR protein expression level, after co-transformation with plasmids pKtac2- teiR and p6, was lower than that observed in the co-transformation with pK teiR100 and p6. The first co-transformation induced 1.20 μg/mg 3α-HSD/CR protein and the second 1.71 μg/mg. These values rose to 1.42 and 1.80 μg/mg, respectively, after treatment with 1 mmol/l IPTG. Our results proved that the tac promoter was more efficient than the lacZ promoter and that the teiR gene could act as an activator for hsdA gene expression.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134397364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1