Journal of Experimental Biology and Agricultural Sciences最新文献_第9页

Haberlea rhodopensis alcohol extract normalizes stress-responsive transcription of the human TP53 gene Haberlea rhodopensis醇提取物使人类TP53基因的应激反应转录正常化

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-04-30 DOI: 10.18006/2023.11(2).405.415

N. Dimitrova, D. Staneva, B. Popov, A. Alexandrova, Milena Georgieva, G. Miloshev

{"title":"Haberlea rhodopensis alcohol extract normalizes stress-responsive transcription of the human TP53 gene","authors":"N. Dimitrova, D. Staneva, B. Popov, A. Alexandrova, Milena Georgieva, G. Miloshev","doi":"10.18006/2023.11(2).405.415","DOIUrl":"https://doi.org/10.18006/2023.11(2).405.415","url":null,"abstract":"The Orpheus flower Haberlea rhodopensis (Friv.) of the family Gesneriaceae can go into anabiosis for long periods in an almost entirely desiccated state. It is an endemic relict from the Balkan Peninsula. Alcohol extracts from H. rhodopensis contain many biologically active substances with potent antioxidant, antigenotoxic, radioprotective, revitalizing and antiaging capabilities. However, regulating the gene networks responsible for these activities is vastly unknown. This study explores the cellular mechanisms underlying the protective effect of H. rhodopensis extracts (HRE). HeLa cells (human cervix epithelial carcinoma, HeLa ATCC® CCL-2™) were used as a model. We examined the changes in catalase activity and TP53 mRNA level shortly after oxidative (H2O2) and ionizing radiation (IR) induced stress with and without pre-incubation with HRE extracts. The dynamics in the activity of catalase, a main cellular antioxidant enzyme, and the expression of the stress-responsive gene TP53 were investigated by UV spectrophotometric assay and RT-qPCR, respectively. Under the applied stress conditions, H2O2 treatment and gamma radiation, catalase activity increased. This was a sign of induced ROS generation. In the first hours after treatment, the two stressors led to opposite changes in the levels of TP53 gene expression, which were alleviated by pre-incubation with HRE in a concentration-dependent manner. The broad biological activities of the studied extract, taking into account our results, show that ability of HRE to reduce the effect of stress is achieved through complex molecular mechanisms aimed at preserving cellular homeostasis. Mechanisms include the normalization of antioxidant enzyme activity such as catalase and the activity of TP53, one of the genes responsive to stress, by up or down-regulation.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43346140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of Regenerative Agriculture Technologies on the Productivity of Cowpea in the Drylands of Embu County, Kenya 再生农业技术对肯尼亚恩布县旱地豇豆生产力的影响

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-02-28 DOI: 10.18006/2023.11(1).190.198

H. Mogaka

{"title":"Effects of Regenerative Agriculture Technologies on the Productivity of Cowpea in the Drylands of Embu County, Kenya","authors":"H. Mogaka","doi":"10.18006/2023.11(1).190.198","DOIUrl":"https://doi.org/10.18006/2023.11(1).190.198","url":null,"abstract":"Cowpea (Vigna unguiculata) is an important indigenous multi-purpose crop grown in arid and semiarid areas of Sub-Saharan Africa (SSA). The cowpea has nutritional and economic value, especially for smallholder farmers in dry lands. However, poor farming practices have declined cowpea productivity over the years. Low soil nutrient replenishment exacerbates the situation, leading to low soil fertility. Uptake of regenerative agriculture (RA) technologies is critical to building more resilient ecosystems that improve soil fertility and agricultural productivity while mitigating climate change effects. This study was carried out to evaluate the impact of the uptake of RA technologies on the productivity of cowpea in the dry lands of Embu County, Kenya. A survey involving 400 farming households was conducted using a semi-structured questionnaire. Descriptive statistics and a stochastic log-linearized Cobb-Douglas production function were used for the data analysis. The study results showed that RA technologies commonly used by farming households were: cereal-legume intercrop, mulching, minimum tillage, crop rotations, pasture cropping, organic agriculture, and compost manure. The findings also revealed that inputs, farm size, labour cost, and used manure amount positively influenced cowpea productivity. The results also showed that cereal-legume intercrop, crop rotations, pasture cropping, and organic agriculture significantly influenced cowpea productivity, while minimum tillage showed a negative relationship. Therefore, the current study's results recommend that the uptake of RA technologies should be scaled to scale up cowpea productivity in dry lands. The study contributes to determining appropriate technologies for cowpea production in arid and semiarid areas. These results will help the government, policymakers, and other inventors to make the right decisions while disseminating or introducing innovations in dry areas.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45710322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors Influencing Suicidal Behaviour among University Students: A Cross-Sectional Study from North India 影响大学生自杀行为的因素:来自北印度的横断面研究

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-02-28 DOI: 10.18006/2023.11(1).54.61

Amrta Tiwari, Nidhish Kumar, Swati Raturi, P. K. Gupta, Mansvee Singh, Hitesh Chopra, Kuldeep Dhama

{"title":"Factors Influencing Suicidal Behaviour among University Students: A Cross-Sectional Study from North India","authors":"Amrta Tiwari, Nidhish Kumar, Swati Raturi, P. K. Gupta, Mansvee Singh, Hitesh Chopra, Kuldeep Dhama","doi":"10.18006/2023.11(1).54.61","DOIUrl":"https://doi.org/10.18006/2023.11(1).54.61","url":null,"abstract":"In the majority of the affected nations, suicidal behavior against COVID-19 leads to various concerns. This study aimed to analyze determinants affecting suicidal behaviour among university students in Uttarakhand. An online cross-sectional survey of 18-year-old university students in Uttarakhand was conducted between April 2 and May 13, 2022. The questionnaire comprised socio-demographic information, the Suicidal Behaviors’ Questionnaire-Revised (SBQ-R) scale, and elements related to the physical and psychological health of COVID-19 (CRPPF). The statistical study included demographic information, basic statistics in terms of frequency and percentage, and logistic regression. In comparison to students with fewer than seven family members, students with more than seven family members were less likely to participate in suicide behaviour (AOR = 2.21; 95% CI: 1.79 to 2.67) and vice versa (AOR = 0.81; 95% CI: 0.56 to 0.97). According to the study, a substantial majority of students (76.35%) claimed that the lockdown implemented to stop the spread of COVID-19 was extremely upsetting for them and that the pandemic had caused them to miss their graduation (73.90%). Adjusted multivariate logistic regression shows that feelings of a burden on family, (AOR= 1.98, 95% CI: 1.09 to 2.82), distancing from family or friends, (AOR =1.66; 95% CI: 1.26 to 2.01), having relationship dilemmas, (AOR= 2.31; 95% CI: 1.84 to 2.97), and being anxious during the lockdown, (AOR= 1.84; 95% CI: 1.08 to 2.27), are significant factors among participants that are linked to higher risk of engaging in suicidal behaviour. The possibility of university students engaging in suicide behaviour was significantly affected by numerous factors. In addition to defending the students' mental health, the concerned authorities should devise and implement strategies to safeguard the students' physical health.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47738855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing the culture conditions for L-Asparaginase production from endophytic fungus Curvularia sp. LCJ413 through conventional and statistical approach 采用常规和统计学方法对内生真菌曲霉LCJ413产l -天冬酰胺酶的培养条件进行优化

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-02-28 DOI: 10.18006/2023.11(1).62.74

Anbarasu Kathiravan, E. Udayan, J. J. Gnanadoss

{"title":"Optimizing the culture conditions for L-Asparaginase production from endophytic fungus Curvularia sp. LCJ413 through conventional and statistical approach","authors":"Anbarasu Kathiravan, E. Udayan, J. J. Gnanadoss","doi":"10.18006/2023.11(1).62.74","DOIUrl":"https://doi.org/10.18006/2023.11(1).62.74","url":null,"abstract":"L-Asparaginase (L-ASNase) is a crucial anti-tumour drug used to cure acute lymphocytic leukaemia. The current study aimed to enhance the production medium for the endophytic fungus Curvularia sp. LCJ413 that showed significant L-ASNase activity. L-ASNase production from Curvularia sp. LCJ413 was examined in six different media to select an appropriate liquid medium. Among the various media tested, Modified Czapek Dox broth (MCDB) exhibited the maximum L-ASNase activity (8.81 ± 0.52 U/mL). Physical (pH and temperature) and nutritional (carbon, nitrogen, inducer, and their concentrations) parameters were also optimized to boost L-ASNase production. Results of the study suggested a temperature of 28°C at pH 7 with 2 g/L maltose, 10 g/L L-Asparagine, and 25 g/L ammonium sulphate as the optimal carbon, inducer, and nitrogen source resulted in a high L-ASNase activity of 18.9 ± 0.40 U/mL. The statistical enhancement of L-ASNase by Response Surface Methodology (RSM) produced 20.11 U/mL of L-ASNase, which was 2.2 fold higher than the non-optimized medium. This is the first study on L-ASNase production from the endophytic Curvularia sp. LCJ413 isolated from Vitex negundo medicinal plant. Continuous fermentation with the medium composition provided in the study can produce L-ASNase on a large scale.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48834282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IRIS-Stage 4 CKD in a Dog: Diagnostic Approaches and Staging of Chronic Kidney Disease: A Case Study 犬iris - 4期CKD:慢性肾脏疾病的诊断方法和分期:一个案例研究

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-02-28 DOI: 10.18006/2023.11(1).216.225

Michael Gunawan, F. Amelia, Ni Nengah Yogiswari Resyana, ZulfaIchsanniyati R.C.F, Siti Zaenab, T. Widyaputri

{"title":"IRIS-Stage 4 CKD in a Dog: Diagnostic Approaches and Staging of Chronic Kidney Disease: A Case Study","authors":"Michael Gunawan, F. Amelia, Ni Nengah Yogiswari Resyana, ZulfaIchsanniyati R.C.F, Siti Zaenab, T. Widyaputri","doi":"10.18006/2023.11(1).216.225","DOIUrl":"https://doi.org/10.18006/2023.11(1).216.225","url":null,"abstract":"Chronic kidney disease (CKD) is a devastating disease of the kidneys that often arise from unresolved acute injury. As a chronic disease, CKD is challenging to diagnose, thus it needs a good combination of a comprehensive understanding of the kidney’s anatomy and physiology and thorough planning for a framework of diagnostic tools to be utilized. This study is intended to provide the diagnostic planning used to determine CKD in an approximately 5-year-old intact male dog that was brought to My Vets Animal Clinic for a check-up visit. On presentation, the dog was emaciated, mildly dehydrated, halitotic, and infested with ticks. A complete blood count (CBC) indicated a normocytic, normochromic, non-regenerative anemia, and lymphopenia. The blood chemistry panel indicated azotemia, elevated symmetric dimethylarginine (SDMA), hypocalcemia, and hyperphosphatemia. Elevated SDMA level (64 μg/dL, reference value: 0-14 μg/dL) and hypercreatinemia (5.9 mg/dL, reference value: 0.5-1.8 mg/dL) indicated impaired glomerular filtration. Physical and clinical pathological findings signified the presence of CKD in this dog, with a stage-4 severity based on International Renal Interest Society (IRIS) CKD staging criteria. The prognosis of this case was highly guarded, and the dog eventually passed away on the sixth day of hospitalization. In a case with an uncertain outcome, accuracy in both diagnosis and staging of CKD in dogs will aid the therapy regimen planning, which may improve the patient’s conditions.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48241298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heavy Metal Tolerance profile among Bacterial species Isolated from Hydrocarbon polluted sites and their mobile genetic elements 烃类污染场所分离细菌的重金属耐受性及其可移动遗传元件

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-02-28 DOI: 10.18006/2023.11(1).158.170

A. Peter, Agbor Reagan Bessong, Iwatt Godwin Dickson, U. S. Emmanuel

{"title":"Heavy Metal Tolerance profile among Bacterial species Isolated from Hydrocarbon polluted sites and their mobile genetic elements","authors":"A. Peter, Agbor Reagan Bessong, Iwatt Godwin Dickson, U. S. Emmanuel","doi":"10.18006/2023.11(1).158.170","DOIUrl":"https://doi.org/10.18006/2023.11(1).158.170","url":null,"abstract":"This present study evaluated the plasmid incidence in bacteria and their genetic elements in heavy metals tolerant-antibiotics resistant microbes isolated from petroleum hydrocarbon polluted sites. The plasmid isolation was carried out using the fermentas Genejet plasmid miniprep kit (Thermofisher Scientific Inc, USA). Screening for class 1, 2, and 3 integrons, incompatibility group P testing, plasmid replicon typing, plasmid restriction analysis, and other analysis was performed using standard laboratory procedures. Plasmid incidences were higher among multiple heavy metal-tolerant bacterial species from hydrocarbon-polluted sites than those from the pristine site. Further, Class 1 integron incidence was significantly higher among the integrons in heavy metal tolerant bacterial isolates isolated from the polluted ecosystems than those from pristine ecosystems. Plasmid replicon type of bacteria with multiple heavy metal tolerance and antibiotics resistance indexes revealed that IncN plasmid replicon type carrying class 1 integron. This encodes resistance to sulphamethazole/trimethoprim, ampicillin, and tolerance to Cd, Ni, and Cu in Klebsiella pneumoniae isolate from petroleum-polluted soil. This is the first report of IncN plasmid in environmental bacteria in Nigeria, particularly from petroleum polluted environment. The conjugation experiment confirmed the possible transferability of antibiotic resistance determinants among isolates in polluted ecosystems. From the results of this study, it can be concluded that petroleum hydrocarbon pollution vis-a-vis heavy metal selective pressure with the abundance of mobile genetic elements amongst isolates from polluted ecosystems could contribute to the dispersing of antibiotic resistance genes, thus posing a serious public health concern.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47148083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant Potential of Chloranthus erectus (Chloranthaceae) from various solvents extract 不同溶剂提取物对直立金花子抗氧化能力的影响

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-02-28 DOI: 10.18006/2023.11(1).75.80

Izzaz Hafiezy Zemry, Nor' Aishah Hasan, N. I. Hasbullah, M. Z. Nawahwi, Azzreena Mohamad Azzeme, Siti Noor Dina Ahmad, Suhaidi Ariffin

{"title":"Antioxidant Potential of Chloranthus erectus (Chloranthaceae) from various solvents extract","authors":"Izzaz Hafiezy Zemry, Nor' Aishah Hasan, N. I. Hasbullah, M. Z. Nawahwi, Azzreena Mohamad Azzeme, Siti Noor Dina Ahmad, Suhaidi Ariffin","doi":"10.18006/2023.11(1).75.80","DOIUrl":"https://doi.org/10.18006/2023.11(1).75.80","url":null,"abstract":"Chloranthus erectus is a herbaceous plant that has been used as a medicinal plant in several regions such as China and Southeast Asia. Although it possesses valuable medicinal properties, till now there is not much research has been carried out on the medicinal properties of this plant and the knowledge of this plant is limited among the research fertility. Therefore, this study aimed to identify the phytochemicals, total phenolic content (TPC), and antioxidant activity of leaf and twig of C. erectus in various solvents extract (hexane, petroleum ether, chloroform, ethyl acetate, and methanol). Phytochemical screening of extracts showed the presence of alkaloids, flavonoids, terpenoids, saponins, quinones, glycosides, and steroids. The highest phenolic content for leaf and twig samples was determined from the methanolic (9.64 ± 0.15 µg GAE/g) and hexanoic extract (7.39 ± 0.27 µg GAE/g), respectively. Meanwhile, the highest antioxidant activity was reported from the methanolic extract of both leaf (88.36 ± 0.24%) and twig (91.25 ± 0.10%) samples. Hence, the results of the study can be concluded that C. erectus has the potential to become a good natural antioxidant and the information from this study can be utilized by the communities as well as other researchers.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45071491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Beneficial impacts of goat milk on the nutritional status and general well-being of human beings: Anecdotal evidence 羊奶对人类营养状况和总体健康的有益影响:轶事证据

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-02-28 DOI: 10.18006/2023.11(1).1.15

Nelson Navamniraj K, S. K, Ankitha Indu J, Deepika Krishnan, Anjali M R, Akhil P R, P. M, F. Nainu, Praveen S V, Prachi Singh, Hitesh Chopra, Sandip Chakraborty, A. Dey, K. Dhama, Deepak Chandran

{"title":"Beneficial impacts of goat milk on the nutritional status and general well-being of human beings: Anecdotal evidence","authors":"Nelson Navamniraj K, S. K, Ankitha Indu J, Deepika Krishnan, Anjali M R, Akhil P R, P. M, F. Nainu, Praveen S V, Prachi Singh, Hitesh Chopra, Sandip Chakraborty, A. Dey, K. Dhama, Deepak Chandran","doi":"10.18006/2023.11(1).1.15","DOIUrl":"https://doi.org/10.18006/2023.11(1).1.15","url":null,"abstract":"Goats provide an essential food supply in the form of milk and meat. Goat milk has distinct qualities, but it shares many similarities with human and bovine milk regarding its nutritional and therapeutic benefits. Because of their different compositions, goat and cow milk products could have different tastes, nutrients, and medicinal effects. Modification in composition aid of goat milk determining the viability of goat milk processing methods. Comparatively, goat's milk has higher calcium, magnesium, and phosphorus levels than cow's or human milk but lower vitamin D, B12, and folate levels. Goat milk is safe and healthy for infants, the old, and healing ailments. Capric, caprylic, and capric acid are three fatty acids that have shown promise as potential treatments for various medical issues. Considering the benefits and drawbacks of goat milk over cow milk is essential; goat milk is more digestible, has unique alkalinity, has a better buffering capacity, and has certain medicinal benefits. Acidifying goat milk shrinks fat globules and makes protein friable (with less αs1-casein and more αs2-casein). Goat milk treats malabsorption illnesses because it has more short- and medium-chain triglycerides that give developing children energy. In wealthy countries, goat milk and its products—yoghurt, cheeses, and powdered goods—are popular with connoisseurs and persons with allergies and gastrointestinal issues who need alternative dairy products. A food product category containing fermented goat milk with live probiotic microbes appears promising nutritionally and medicinally. This article presents anecdotal evidence of the therapeutic effects of consuming goat milk for human health and its nutritional value.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44490805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Optimization of Total Flavonoid Extraction From the Helicteres hirsuta Lour. Roots by Bath Ultrasound Assisted method and cytotoxic activities of these Flavonoids 毛冬青总黄酮提取工艺的优化。浸浴超声辅助生根及其黄酮类化合物的细胞毒性研究

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-02-28 DOI: 10.18006/2023.11(1).179.189

Tong Thi Hue, Dang Thanh Long, Van Thi My Le, Ho Ngoc Han, Nguyen Thi Quynh Trang

{"title":"Optimization of Total Flavonoid Extraction From the Helicteres hirsuta Lour. Roots by Bath Ultrasound Assisted method and cytotoxic activities of these Flavonoids","authors":"Tong Thi Hue, Dang Thanh Long, Van Thi My Le, Ho Ngoc Han, Nguyen Thi Quynh Trang","doi":"10.18006/2023.11(1).179.189","DOIUrl":"https://doi.org/10.18006/2023.11(1).179.189","url":null,"abstract":"This study was carried out to optimize the various approaches to analyze the effects of various variables on the total flavonoid content extraction from the roots of Helicteres hirsuta L. The existence of various compounds in the methanol fraction was accessed by using LC-MS/MS analysis. The results of the study identified the ideal parameters such as times (30 minutes); methanol solvent concentration (50%); ultrasonic frequency (12 Hz); and material/solvent ratio [1:30 (w/v)] for extracting the highest total flavonoids from the roots of H. Hirsuta. The study's results suggested that the total flavonoid value was 3.52684 (mg Catechin/g extract). The verified experiment obtained an actual value of 5.205 (mg Catechin/g extract). Further, the results of the study suggested the presence of 20 compounds of a flavonoid nature (66.667%) appearing in the purified methanol fractional extract. These compounds can inhibit DPPH free radicals at 50%, with an IC50 value of 536.760 g/mL, and they also have inhibitory activity on the growth of cancer cell lines with IC50 values ranging from 115.81 and 219.17g/mL. The human leukemia cell line (HL-60) exhibits the most significant cytotoxic response to a methanol extract from H. hirsuta root with an IC50 value of 115.81 g/mL.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44410382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Productivity and profitability of commercial broiler chickens under various farming conditions 商品肉鸡在不同养殖条件下的生产力和盈利能力

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-02-28 DOI: 10.18006/2023.11(1).209.215

Sharif Uddin Khan, S. K. Fouzder, P. Sarkar

{"title":"Productivity and profitability of commercial broiler chickens under various farming conditions","authors":"Sharif Uddin Khan, S. K. Fouzder, P. Sarkar","doi":"10.18006/2023.11(1).209.215","DOIUrl":"https://doi.org/10.18006/2023.11(1).209.215","url":null,"abstract":"Broiler farming plays a vital role in fulfilling global protein requirements. Although broiler farming is considered profitable, profitability might be affected by factors such as genetics, feed quality, and management practices. In the current study, the productivity and profitability of commercial broiler farming were studied under various farming conditions, such as farm size, location of the farm, and mortality of the broilers. Data were collected through farmers' interviews and farm record books and processed and analyzed to determine the productivity and profitability of broiler farming. Productivity and profitability did not differ significantly across farm sizes and locations. The results of the study reported mortality as a factor affecting productivity and profitability in broiler farming. It was manifest that mortality adversely affected the productivity and profitability of broiler farming. A significant positive relationship was recorded between mortality and feed conversion ratio. Moreover, mortality was negatively correlated with the gross margin of broiler farming, meaning that the low gross margin was due to the high mortality at broiler farms. The farms were more profitable when the mortality was <5%, compared to >10%. It is recommended to reduce the mortality percentage of broiler chickens as minimum as possible, preferably <5%. Good quality chicks, better management, and the prevention of diseases might play an important role in keeping the mortality rate at a minimum level in broiler farming.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47819382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2