Journal of Experimental Biology and Agricultural Sciences最新文献_第4页

Work Place Self-Management by Academic Cum Clinical Physiotherapist – A Case Study 学术暨临床物理治疗师的工作场所自我管理--案例研究

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-11-30 DOI: 10.18006/2023.11(5).866.870

Vinodhkumar Ramalingam, Mythri Jain, P. Suganthirababu, J. Alagesan, Pradeep Balakrishnan, Prathibha Duraimurugan, Balarathna, Mohammed Hussain, Wong Ling Shing

{"title":"Work Place Self-Management by Academic Cum Clinical Physiotherapist – A Case Study","authors":"Vinodhkumar Ramalingam, Mythri Jain, P. Suganthirababu, J. Alagesan, Pradeep Balakrishnan, Prathibha Duraimurugan, Balarathna, Mohammed Hussain, Wong Ling Shing","doi":"10.18006/2023.11(5).866.870","DOIUrl":"https://doi.org/10.18006/2023.11(5).866.870","url":null,"abstract":"One of the main issues in industrialized nations is work-related musculoskeletal disorders (WMSD), which are linked to demanding and repetitive work environments. Repetitive actions and work without leisure can lead to musculoskeletal problems. To prevent this occurrence, promoting healthy lifestyle self-care management is essential. The main objective of this case study was to assess the risk of work-related musculoskeletal disorders in professional physiotherapist and their workplace self-management. Case Description: a case of a 39-year-old male assistant professor cum clinical practitioner with 12 years of experience in the profession, who engages in using the computer for 6 hours a day for his academic and clinical documentation purposes, was assessed in the workstation. He complains of neck and shoulder pain after engaging in front of the computer for more than one hour, which he manages during the routine practice. He manages his pain by doing simple exercises like chin tuck-in and shoulder bracing by taking a short break every hour while working on a computer. Musculoskeletal-related pain was analyzed using the VAS score (visual analogue scale), and the level of the risks was analyzed using the rapid entire body assessment (REBA) scale. The case study showed that professional physiotherapists have a high risk of MSDs due to their engagement in multiple tasks and less attention to body posture. Regular exercise in the workstation and ergonomic workplace awareness would prevent the occurrence of work-related MSD.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"579 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139204401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of olive mill waste-based biochar for improving wheat response to salt stress 应用以橄榄油厂废料为基础的生物炭改善小麦对盐胁迫的反应

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-11-30 DOI: 10.18006/2023.11(5).854.865

H. Abou-Zeid, Hesham M. Aly

{"title":"Application of olive mill waste-based biochar for improving wheat response to salt stress","authors":"H. Abou-Zeid, Hesham M. Aly","doi":"10.18006/2023.11(5).854.865","DOIUrl":"https://doi.org/10.18006/2023.11(5).854.865","url":null,"abstract":"The production of olive mill solid waste (OMSW) raises concerns due to its toxicity and negative environmental impact. However, by utilizing pyrolysis, OMSW can be converted into biochar, a carbon-rich material that detoxifies the waste and preserves its nutrient content. The OMSW-based biochar possesses alkaline properties (pH 9.6), low electrical conductivity (EC), high cation exchange capacity (CEC), a porous surface morphology, various surface functional groups, and high mineral content. This study assessed the influence of two concentrations (5% and 10%) of OMSW-based biochar on wheat plants' growth biomarkers and physiological characteristics subjected to salt stress conditions (150 mM NaCl). Findings of the study revealed that salt stress had deleterious effects on various parameters, including shoot height, fresh and dry weights of shoots and roots, relative water content (RWC%), membrane stability index (MSI%), photosynthetic pigments, and photosynthetic parameters such as the coefficient of the effective quantum yield of photochemical energy conversion of PSII (ØPSII), photochemical quenching (qP), and photochemical efficiency of PSII (Fo, Fm, Fv/Fo, and Fv/Fm). Furthermore, the levels of lipid peroxidation (MDA), hydrogen peroxide (H2O2), superoxide dismutase (SOD), and peroxidase (POD) activities significantly increased in stressed plants. On the other hand, applying both concentrations of OMSW-based biochar effectively improved the overall performance of wheat plants, irrespective of the presence of salinity. OMSW-based biochar is a promising strategy for promoting wheat growth in salt-stressed soil by improving various growth parameters and mitigating plant oxidative stress.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139207274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient Solar-Powered IoT Drip Irrigation for Tomato Yield and Quality: An Evaluation of the Effects of Irrigation and Fertilizer Frequency 高效太阳能物联网滴灌提高番茄产量和质量：灌溉和施肥频率的影响评估

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-11-30 DOI: 10.18006/2023.11(5).845.853

Sanya Kaunkid, A. Aurasopon

{"title":"Efficient Solar-Powered IoT Drip Irrigation for Tomato Yield and Quality: An Evaluation of the Effects of Irrigation and Fertilizer Frequency","authors":"Sanya Kaunkid, A. Aurasopon","doi":"10.18006/2023.11(5).845.853","DOIUrl":"https://doi.org/10.18006/2023.11(5).845.853","url":null,"abstract":"The optimal management of irrigation and fertilization is crucial for maximizing the yield and quality of tomatoes grown in greenhouses. To address this challenge, this study aimed to develop and implement a solar-powered Internet of Things (IoT) based drip irrigation system for tomato cultivation in plastic roof net houses. Additionally, the study evaluated the effects of water and fertilizer frequency on tomato yield and quality. The experiment was designed with 2 irrigation frequencies (1 time in a day and 1 time in 2 days) and 3 fertilizer frequencies (1 time in 2, 4, and 6 days), with 4 replicates of the tomato variety CH154. The results showed that the solar-powered IoT-based drip irrigation system was efficient, precise in water and fertilizer control, and inexpensive to install and maintain. This allows for real-time monitoring of water flow rate, flow sensor status, treatment status, and electrical parameters on the Node-Red dashboard. Irrigation frequency had a significant impact (p < 0.05) on fruit number, weight, and length per plant, with 1-day irrigation resulting in a higher yield than 2-day irrigation. No significant interaction effect was found between irrigation and fertilizer frequency on tomato yield or quality. In conclusion, the solar-powered IoT-based drip irrigation system demonstrated precise control over water and fertilizer, proving its efficiency and cost-effectiveness. Real-time monitoring capabilities and the observed impact of irrigation frequency underscore its potential for enhancing tomato cultivation in greenhouses, offering a valuable contribution to sustainable and technology-driven agricultural practices.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139207484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Halotolerant Plant Growth Promoting Bacilli from Sundarban Mangrove Mitigate the Effects of Salinity Stress on Pearl Millet (Pennisetum glaucum L.) Growth 孙德班红树林耐盐促生杆菌缓解盐胁迫对珍珠粟(Pennisetum glaucum L.)的影响增长

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-08-31 DOI: 10.18006/2023.11(4).746.755

None Pallavi, Rohit Kumar Mishra, Ajit Varma, Neeraj Shrivastava, Swati Tripathi

{"title":"Halotolerant Plant Growth Promoting Bacilli from Sundarban Mangrove Mitigate the Effects of Salinity Stress on Pearl Millet (Pennisetum glaucum L.) Growth","authors":"None Pallavi, Rohit Kumar Mishra, Ajit Varma, Neeraj Shrivastava, Swati Tripathi","doi":"10.18006/2023.11(4).746.755","DOIUrl":"https://doi.org/10.18006/2023.11(4).746.755","url":null,"abstract":"Pearl millet (Pennisetum glaucum L.) is one of the major crops in dry and saline areas across the globe. During salinity stress, plants encounter significant changes in their physio and biochemical activities, leading to decreased growth and yield. Bacillus species are used as biofertilizers and biopesticides for pearl millet and other crops to promote growth and yield. The use of Bacillus in saline soils has been beneficial to combat the negative effect of salinity on plant growth and yield. In this context, the present study emphasizes the use of two Bacillus species, i.e. Bacillus megaterium JR-12 and B. pumilus GN-5, which helped in alleviating the impact of salinity stress on the growth activities in salt-stressed pearl millet. Pearl millet seeds were treated with two strains, B. megaterium JR-12 and B.pumilus GN-5, individually and in combination under 50, 100 and 150 mM of sodium chloride stress. The treated plants showed higher plant height, biomass accumulation, and photosynthetic apparatus than the non-treated plants. Additionally, the treated plants showed increased osmoprotectant levels under salinity stress compared to control plants. The antioxidant enzyme content was improved post-inoculation, indicating the efficient stress-alleviating potential of both strains of Bacillus species. Moreover, inoculation of these microbes significantly increased plant growth attributes in plants treated with a combination of Bp-GN-5 + Bm-JR-12 and the reduction rates of plant growth were found to be alleviated to 9.12%, 20.30% and 33%, respectively. Overall, the results of the present study suggested that these microbes could have a higher potential to improve the productivity of pearl millet under salinity stress.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"2015 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135992322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of forest conversion to oil palm plantation on soil erosion and surface runoff 林改油棕对土壤侵蚀和地表径流的影响

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-08-31 DOI: 10.18006/2023.11(4).767.779

Adi Jaya, None Salampak, Nyahu Rumbang, Mofit Saptono, Lusia Widiastuti, Sri Endang Agustina Rahayuningsih, Shella Winerungan

{"title":"Effects of forest conversion to oil palm plantation on soil erosion and surface runoff","authors":"Adi Jaya, None Salampak, Nyahu Rumbang, Mofit Saptono, Lusia Widiastuti, Sri Endang Agustina Rahayuningsih, Shella Winerungan","doi":"10.18006/2023.11(4).767.779","DOIUrl":"https://doi.org/10.18006/2023.11(4).767.779","url":null,"abstract":"The vegetation type and its coverage in forest ecosystems are crucial in soil erosion and surface runoff. Cover crops provide significant protection to the soil aggregates, preventing damage caused by rainfall and runoff that might occur in the absence of these crops. However, changes in land use, such as converting forests into oil palm plantations, have resulted in changes to the land cover, which affect erosion, surface runoff, and, ultimately, the forest ecology of the watershed. This study aimed to provide an overview of erosion and runoff in forest areas and oil palm plantations. This field research was conducted to study erosion, runoff, and nutrient loss using plots measuring 15m x 25m, including oil palm plantation areas and forest areas. After each rain, sediment weighing and runoff volume measurements were carried out. Laboratory analysis was conducted for sediment and surface runoff water samples’ N, P, and K elements. The study results showed that five-year-old oil palm plantation areas experience the highest levels of erosion and runoff, followed by three-year-old oil palm plantation and forest areas. Nutrients were found to be lost in sediment across all land cover types, with a minimal amount recorded in surface runoff.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135992332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Length-Weight Relationship and Condition Factor of Oreochromis niloticus (Linnaeus, 1758) in Selected Tropical Reservoirs of Ekiti State, Southwest Nigeria 尼日利亚西南部Ekiti州热带水库Oreochromis niloticus (Linnaeus, 1758)的长权关系及条件因子

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-08-31 DOI: 10.18006/2023.11(4).707.719

Olagbemide P. T., Owolabi O. D.

{"title":"Length-Weight Relationship and Condition Factor of Oreochromis niloticus (Linnaeus, 1758) in Selected Tropical Reservoirs of Ekiti State, Southwest Nigeria","authors":"Olagbemide P. T., Owolabi O. D.","doi":"10.18006/2023.11(4).707.719","DOIUrl":"https://doi.org/10.18006/2023.11(4).707.719","url":null,"abstract":"In Ekiti State, southwest Nigeria, Oreochromis niloticus is significant to live. This investigation was conducted to improve its sustainable management and access the length-weight relationship and condition factor of O. niloticus across important reservoirs in Ekiti State from November 2017 to October 2019. Collected specimens were weighed to the nearest gram, while the entire lengths were measured to the closest centimetre. Log-transformed regression was used to determine the fish's growth pattern in the reservoirs. The gradient comparison was done using the T-test. The length and weight of the species in the reservoirs showed a significant association. All length-weight relationships had r2 values greater than 0.8150 and were significant at P < 0.05. In the length-weight relationship of O. niloticus, the values of the exponent b in Egbe, Ero, and Ureje reservoirs varied from 2.45 to 2.87, 3.02 to 3.20, and 2.45 to 2.82, respectively. The results of regression coefficient b obtained showed that in the combined season, male, female and combined sexes from the Ero reservoir had isometric growth patterns with growth exponent b values of 3.18, 3.20 and 3.19 respectively that were not statistically different from 3. These results contradicted the negative allometric growth pattern in the Egbe and Ureje reservoirs. The condition factor during the dry season ranged from 1.93-2.05, 2.05-2.11, and 1.93-2.03 for fish in Egbe, Ero, and Ureje reservoirs, respectively while in the rainy season, it ranged from 2.00-2.02; 2.08-2.09 and 1.96-2.01 respectively. The fish species studied in the reservoirs lived above- average life and thus indicate that the prevailing ecological conditions in the reservoirs were not beyond the forbearance range for the fish species since their condition factors were within the range considered adequate for freshwater fishes in tropical waters.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135992326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the Nucleotide and Turmeric Extract Supplementation and different Cage Floors on the Blood Profile and Physiological Status of Broiler Chicken 添加核苷酸和姜黄提取物及不同笼底对肉仔鸡血液特征和生理状态的影响

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-08-31 DOI: 10.18006/2023.11(4).696.706

Elly Tugiyanti, None Ismoyowati, None Rosidi, Dadang Mulyadi Saleh, Soegeng Heriyanto, Tri Laras Wigati

{"title":"Effect of the Nucleotide and Turmeric Extract Supplementation and different Cage Floors on the Blood Profile and Physiological Status of Broiler Chicken","authors":"Elly Tugiyanti, None Ismoyowati, None Rosidi, Dadang Mulyadi Saleh, Soegeng Heriyanto, Tri Laras Wigati","doi":"10.18006/2023.11(4).696.706","DOIUrl":"https://doi.org/10.18006/2023.11(4).696.706","url":null,"abstract":"Climate change has been responsible for the high prevalence of heat stress (HS) among broiler chickens. In this research, efforts are made to curb the negative impact of HS on chickens by modifying the feed and cage floor. The blood profile and physiological responses of broiler chickens supplemented with nucleotide and turmeric powder and kept in different floor cages were recorded (litter, slatted, and combination of slat-litter). A total of 245 broiler day-old chicks (DOC) were randomly allotted to seven treatment groups of the combined supplementation of nucleotide and turmeric extract and different types of cage floor (litter, slate, combination of slat-litter) for 35-day maintenance. Each treatment was replicated five times. The supplementation of nucleotide and turmeric extract into feed and different types of cage floor did not significantly affect (P>0.05) body temperature, respiratory rate, pulse rate, lien index, PVC, TPP, heterophils, lymphocyte, and monocyte, but significantly affected (P<0.05) the erythrocyte level, hemoglobin, leukocyte, rectal temperature and the index of bursa fabricius of broilers. Results of this study concluded that the combined treatments of supplementing nucleotide and turmeric extract in feed and using slat-floored cages tend to reduce the comfort of broiler chickens.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135992329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of nutrient management on physio morphological and yield attributes of field pea (Pisum sativum L.) 营养管理对大田豌豆生理形态及产量性状的影响

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-08-31 DOI: 10.18006/2023.11(4).736.745

Reguri Harsha Vardhan Reddy, Arshdeep Singh, Anita Jaswal, Shimpy Sarkar, Iza Fatima

{"title":"Effect of nutrient management on physio morphological and yield attributes of field pea (Pisum sativum L.)","authors":"Reguri Harsha Vardhan Reddy, Arshdeep Singh, Anita Jaswal, Shimpy Sarkar, Iza Fatima","doi":"10.18006/2023.11(4).736.745","DOIUrl":"https://doi.org/10.18006/2023.11(4).736.745","url":null,"abstract":"A field experiment was conducted to investigate the impact of nitrogen management on the growth and yield of field peas. The experiment took place during the rabi season (October–March of 2022–2023) at Lovely Professional University's Agriculture Research Farm in Phagwara, Punjab, India. Fifteen different treatment combinations were utilized, involving the application of chemical fertilizers (NPK) and micronutrients (boron and zinc). The experimental design followed a randomized complete block approach with three replications. Among the treatment combinations, the application of foliar spray with B at a rate of 0.2%, Zn at a rate of 0.5%, along with 100% recommended dose of fertilizer (RDF), resulted in the highest measurements for plant height (70.44 cm), leaf count (70.60), branch count (18.86), leaf area (32.24 cm²), dry matter accumulation (6.12 g), crop growth rate (0.299 g m⁻² day⁻¹), and relative growth rate (0.05933 g g⁻¹ day⁻¹). Furthermore, treatments involving 100% RDF, 0.2% B, and 0.5% Zn exhibited enhanced yield characteristics, including the number of seeds per pod (10.26), pods per plant (12.33), test weight of seeds (15.06 g), seed yield (3537 kg ha⁻¹), and harvest index (47.49%). Furthermore, 100% RDF and the inclusion of 0.2% B and 0.5% Zn outperformed the control. Applying 100% RDF along with the micronutrients B and Zn is recommended to maximize production and net profit in field pea cultivation.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135992327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acid Rain and Seed Germination: A Predictive Model Using ML-based CART Algorithm 酸雨与种子萌发:基于ML-based CART算法的预测模型

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-08-31 DOI: 10.18006/2023.11(4).720.735

Vasundhara Arora, Bikram Jit Singh, Navneet Bithel, Tapan Kumar Mukherjee, Sushil Kumar Upadhyay, Rippin Sehgal, Raj Singh

{"title":"Acid Rain and Seed Germination: A Predictive Model Using ML-based CART Algorithm","authors":"Vasundhara Arora, Bikram Jit Singh, Navneet Bithel, Tapan Kumar Mukherjee, Sushil Kumar Upadhyay, Rippin Sehgal, Raj Singh","doi":"10.18006/2023.11(4).720.735","DOIUrl":"https://doi.org/10.18006/2023.11(4).720.735","url":null,"abstract":"The impact of acid rain on the germination of seeds is a significant concern in agricultural and environmental studies. Acid rain, characterized by elevated acidity levels due to pollutants like sulfur dioxide and nitrogen oxides, can adversely affect the germination process of various plant species. The objective of this study was to evaluate the impact of simulated acid rain (SAR) on the germination of Brinjal (Solanum melongena Linn.) and Cowpea (Vigna unguiculata ssp. cylindrica L. Walpers) crops. The experiments were conducted using eight plastic trays of approximately 25 cm. x 30 cm dimensions. Four trays were used for experiments with brinjal seeds (Set I), while the other four were used for cowpea seeds (Set II). One tray from each set was used as positive control and treated with normal pH 5.6, while the other three trays from each batch were treated with SAR solutions of pH 4.5, 3.5, and 2.5. Brinjal seed germination percentage and seed vigor were inferior to Cowpea seeds. The seeds treated with SAR (pH 4.5, 3.5, and 2.5) showed hindered seed germination. Furthermore, a more significant inhibitory effect was observed at lower pH values. The mean germination percentage of seeds was highest for standard SAR (pH 5.6) in the case of Brinjal seeds, while it was recorded lowest for Cowpea seeds. The results indicate that plants do not respond uniformly to SAR. To investigate the behavior of the simulated acid rain data, a Machine Learning-based Decision Tree Algorithm was employed to identify and optimize conditions. Cowpea was predicted to get 95% seed germination, whereas brinjal would only be 64% in acid rain of pH value 5.05 for 36 hours. In conclusion, utilizing a Machine Learning-based CART algorithm has provided valuable insights into predicting the germination behavior of seeds under the influence of acid rain.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135992328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of human β-Globin second intron on transient gene expression in mammalian cell lines 人β-珠蛋白第二内含子对哺乳动物细胞系瞬时基因表达的影响

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2023-08-31 DOI: 10.18006/2023.11(4).663.670

Kevin Kumar Vijayakumar, Humera Khathun Abdul Hameed, Shakila Harshavardhan

{"title":"Effect of human β-Globin second intron on transient gene expression in mammalian cell lines","authors":"Kevin Kumar Vijayakumar, Humera Khathun Abdul Hameed, Shakila Harshavardhan","doi":"10.18006/2023.11(4).663.670","DOIUrl":"https://doi.org/10.18006/2023.11(4).663.670","url":null,"abstract":"Exogenous protein expression in mammalian cells is necessary to produce therapeutic proteins and modern medical applications like developing DNA vaccines and gene therapy. This study examines the human-Globin (hBG) second intron's capacity for intron-mediated enhancement (IME) in various mammalian cell lines. Our study's main aim is to investigate the effect of the incorporation and arrangement of the second intron of the human Beta-globin gene into the pVAX-1 expression cassette on improving the expression of foreign genes. Two plasmids were constructed, one with the hBG second intron positioned upstream and the other downstream in the expression cassette. EGFP expression was evaluated at the mRNA and protein levels after transfection using Lipofectamine 2000 using One-way ANOVA analysis. Results showed that the pVAX-1 harbouring the hBG second intron did not lead to enhanced transient EGFP expression and did not exhibit Intron Mediated Enhancement (IME) in tested mammalian cell lines. Further investigations are necessary to understand factors contributing to the lack of enhancement and explore alternative intron options for optimizing foreign gene expression in cell lines.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135992325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0