Journal of Chinese medicinal materials Pub Date : 2016-09-01

Yu-lin Wei, Shu-mei Wang, Qi-ou Liu, Yang Luo

{"title":"[Study on the Mechanism of Yiqi Chutan Recipe in the Treatment of Lung Cancer with Splenasthenic Syndrome].","authors":"Yu-lin Wei, Shu-mei Wang, Qi-ou Liu, Yang Luo","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the mechanism of Yiqi Chutan recipe( YCR) in the treatment of lung cancer with splenasthenic syndrome from the perspective of the endoplasmic reticulum stress( ERS).Methods: 60 mice were successfully modeled as Lewis lung cancer mice with spleen deficiency, which were randomly divided into model group( normal saline),cisplatin group( 1 mg / kg),low dose( 2 g/kg) YCR group, middle dose( 4 g/kg) YCR group,high dose( 8 g/kg) YCR group and the combined medication group( YCR 4 g/kg + cisplatin 1 mg / kg),with 10 mice in each group. The mice were killed after 14 days,the size and weight of tumor in mice were measured,morphological changes of tumor were observed by HE staining method, the expression of CHOP and DR5 protein in tumor tissue was detected by immunohistochemical method and Western blot method.Results: Compared with model group, the volume and weight of tumor in cisplatin group, middle dose YCR group, high dose YCR group and combined medication group were significantly decreased( P < 0. 01),and the tumor cells in transplanted tumor were large necrosis and the protein expression of CHOP and DR5 were increased significantly( P < 0. 01). The effect in treatment of lung cancer with splenasthenic syndrone in combined medication group was higher( P < 0. 05 or P < 0. 01).Conclusion: Yiqi Chutan recipe has different degrees of inhibition on lung cancer, the combined use of cisplatin can obviously improve the curative effect. The mechanism may be related to up-regulating the expression of CHOP and DR5 in endoplasmic reticulum stress-related proteins.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36486637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Chemical Constituents of Lonicerae Japonicae Flos]. 【金银花的化学成分】。

Journal of Chinese medicinal materials Pub Date : 2016-09-01

Xu Wang, Hong-lei Zhou, Rong Rong

引用次数: 0

[HPLC Fingerprint and Determination in the Root of Amorpha fruticosa]. 紫穗槐根的HPLC指纹图谱及含量测定

Journal of Chinese medicinal materials Pub Date : 2016-09-01

Yu-wei Zhao, Miao Yu, Min-lun Nan, Na Lv, Xue-ling Si, Yu-fang He

{"title":"[HPLC Fingerprint and Determination in the Root of Amorpha fruticosa].","authors":"Yu-wei Zhao, Miao Yu, Min-lun Nan, Na Lv, Xue-ling Si, Yu-fang He","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To establish HPLC fingerprint in the root of Amorpha fruticosa, and simultaneously to determine the content of calycosin-7-O-β-D-glucopyranoside, ononin, calycosin, formononetin.Methods: The analytical column was Diamonsil C18( 250 mm ×4. 6 mm,5 μm). The mobile phase was acetonitrile( A)-water( B)( containing 0. 2% phosphoric acid) in gradient elution, and the detection wavelength was set at 260 nm. \"Chromatographic fingerprint similarity evaluation software \"version( 2004A) was used to evaluate similarity for the ten batches medicinal materials,and SPSS software was used for cluster analysis.Results: The HPLC fingerprint of the root of Amorpha fruticosa was established with good separation, and four chemical compositions were determinated. 16 common peaks were defined in the HPLC fingerprint among the 10 batches of the root of Amorpa fruticosa. The similarity among them was more than0. 90.Conclusion: This analytical method has strong features,with a good repeatability and the method is simple, which can be used efficiently in the quality control in the root of Amorpha fruticosa.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36487244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Chemical Constituents from Eucommia ulmoides]. 杜仲的化学成分。

Journal of Chinese medicinal materials Pub Date : 2016-09-01

Kun Li, Zhi-you Hao, Cui-li Zhang, Yan-jun Sun, Hui Chen

引用次数: 0

[Sodium Aescinate Induced Apoptosis of BGC-823 and AGS Cells by Inhibiting JAK-1/STAT-1 Signaling Pathway]. 七叶皂苷钠通过抑制JAK-1/STAT-1信号通路诱导BGC-823和AGS细胞凋亡

Journal of Chinese medicinal materials Pub Date : 2016-09-01

{"title":"[Sodium Aescinate Induced Apoptosis of BGC-823 and AGS Cells by Inhibiting JAK-1/STAT-1 Signaling Pathway].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the mechanism of sodium aescinate on the apoptosis of gastric cancer BGC-823 cells and AGS cells.Methods: CCK-8 assay was used to detect the cell viability of cancer cells; cells morphological changes were observed by inverted microscope; changes in nuclear morphology were observed by fluorescence inverted microscope after DAPI staining; cells apoptosis rate was detected by flow cytometry; Western blotting was used to detected the phosphorylation of JAK-1 and STAT-1; laser confocal microscopy was used to observed the nuclear translocation of STAT-1.Results: Sodium aescinate inhibited cells proliferation in a concentration dependent manner, and changed the cells morphology and nuclear morphology, significantly up-regulated the apoptosis rate of gastric cancer BGC-823 cells and AGS cells, and down-regulated the phosphorylation of JAK-1, STAT-1 protein, inhibited the nuclear translocation of STAT-1.Conclusion: Sodium aescinate inhibit the proliferation and induce apoptosis in the gastric cancer BGC-823 cells and AGS cells, and this process can be implemented by inhibiting the JAK-1/STAT-1 signaling pathway.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36487089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Chemical Constituents of Alkaloids from the Bark of Sophora japonica]. [苦参树皮生物碱的化学成分]。

Journal of Chinese medicinal materials Pub Date : 2016-09-01

Long Pan, Man Zhang, He-ru Chen

引用次数: 0

[Effect of Different Locations and Genotypes on Yield and Accumulation of Bioactive Constituents in Salvia miltiorrhiza]. [不同位置和基因型对丹参产量和生物活性成分积累的影响]。

Journal of Chinese medicinal materials Pub Date : 2016-09-01

Qi Zhao, Yu-ling Pan, Hai-tao Dou, Jun-heng Hua, Xin-xin Fu, Jian-hua Wang

{"title":"[Effect of Different Locations and Genotypes on Yield and Accumulation of Bioactive Constituents in Salvia miltiorrhiza].","authors":"Qi Zhao, Yu-ling Pan, Hai-tao Dou, Jun-heng Hua, Xin-xin Fu, Jian-hua Wang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: The root yield and active constituent contents were analyzed from four Salvia miltiorrhiza cultivars grown at three different locations( Zhuyang, Changqing, and Taian, Shandong Province) to determine the influence of environmental conditions and cultivars. .Methods: Phenolic acids and tanshinones were analyzed by HPLC method. Total phenolic acids content were analyzed by FolinCiocalteu method. Klason method was used to determine the content of lignin.Results: The root yield and the active constituent contents were significantly affected by different environments and cultivars of Salvia miltiorrhiza. The root yield was negatively correlated with active constituent contents. Salvia miltiorrhiza of Zhuyang location had the highest active constituent content, but it had the lowest root yield. Salvia miltiorrhiza of Taian location had the lowest active constituent contents, while it had the highest root yield. Salvia miltiorrhiza of Changqing location had relatively higher bio-yields of phenolic acids and tanshinones, which made it suitable for Salvia miltiorrhiza cultivation. Furthermore, compared with three other cultivars,105 cultivar could remain the salvianolic acid B stable, which indicating that 105 cultivar was possible related to resistances.Conclusion: The research provides a theoretical basis for the selecting of the optimal cultivar and the optimal environmental condition.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36480452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Analysis of Volatile Components and Special Aroma in Fresh Roots of Codonopsis Radix]. 鲜党参根挥发性成分及特殊香气分析

Journal of Chinese medicinal materials Pub Date : 2016-09-01

{"title":"[Analysis of Volatile Components and Special Aroma in Fresh Roots of Codonopsis Radix].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To analyze volatile components and special aroma in fresh roots of Codonopsis Radix.Methods: Volatile components in fresh Codonopsis Radix origined from three plant sources were analyzed by HS-GC-MS, and n-hexanal, 2-hexenal and n-hexanol were quantified by HS-GC.Results: 64 constituents have been identified in Codonopsis Radix,which containing ten alcohol,20 aldehyde,14 terpene, three ketone, four ester, four furan, three acid, one olefin, one alkane, two sulfide and two other compounds. The total percentage of n-hexanal,2-hexenal and n-hexanol was large, which were the main volatile compounds in Codonopsis Radix. The content of n-hexanal in Lu Dangshen, Baitiao Dangshen, Wen Dangshen, Feng Dangshen and Banqiao Dangshen were 54. 89%,59. 01%,55. 07%,42. 42% and 56. 74%,which were the major volatile component contributing to special aroma in Codonopsis Radix. 61 compounds have been identified in Lu Dangshen including 8 specific compounds. An external calibration method was established for simultaneous determination of n-hexanal,2-hexenal and hexanol in Codonopsis Radix. The results showed that all calibration curve three volatile components exhibited good linearity at a range of concentrations( R2≥0. 9995). The recoveries of n-hexanal, 2-hexenal and hexanol were 100. 1%,94. 8% and 112. 6%,and the RSD were 4. 4%,8. 7% and 8. 1%,respectively. The analysis suggested that the content of n-hexanal in Lu Dangshen was 200. 41 μg / g and significantly higher than that in Banqiao Dangshen, Wen Dangshen and Feng Dangshen while similar to Baitiao Dangshen. The content of hexanol in three-year-old Lu Dangshen was significantly higher than that in twoyear-old Lu Dangshen.Conclusion: The present research indicates that special aroma is an important feature of authentic Dangshen.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36484399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Xanthones from Rhizoma of Securidaca inappendiculata]. 【黄原胶中的黄原胶】。

Journal of Chinese medicinal materials Pub Date : 2016-09-01

Qi-wen Wang, Cheng-yao Ma, Dong-dong Chen, Xiang Li, Jian-wei Chen

引用次数: 0

[Study on the Flavonoids and Biological Activity of Rubus sachalinensis]. [沙沙林草总黄酮及其生物活性研究]。

Journal of Chinese medicinal materials Pub Date : 2016-09-01

Shan-shan Ren, Bao-quan Bao

{"title":"[Study on the Flavonoids and Biological Activity of Rubus sachalinensis].","authors":"Shan-shan Ren, Bao-quan Bao","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the chemical constituents and biological activity of Rubus sachalinensis.Methods: The compounds in the ethyl acetate and n-butanol fraction were isolated and purified by macroporous resin,Sephadex LH-20 column chromatography,and reverse-phase preparative HPLC. The structures were identified by various spectroscopic data such as 1D,2D-NMR,HRMS. Antioxidant,anti-inflammatory and cytotoxicity of the compounds were determined by FRAP,Griess and MTT methods.Results: Eight compounds were isolated from the ethyl acetate and n-butanol extract of Rubus sachalinensis,and identified as kaempferol-3-O-β-D-butyl glucuronate( 1),quercetin-3-O-β-D-butyl glucuronate( 2),quercetin-3-O-β-D-glucopyranoside( 3),cis-tiliroside( 4),tiliroside( 5),quercetin-3-O-β-D-ethyl glucuronate( 6),quercetin( 7) and kaempferol( 8). Activity assay showed that the compounds 1 ~ 8 had different levels of antioxidant activity,all the compounds showed cytotoxicities of IC50 greater than 200 μmol / L.Conclusion: Flavonoids are the main antioxidant consitituents of Rubus sachalinensis and have a good anti-inflammatory activity. All the compounds are isolated from this plant for the first time,compounds 1,2,6 are obtained from the this genus for the first time.","PeriodicalId":15312,"journal":{"name":"Journal of Chinese medicinal materials","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36487239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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