Eurasian Journal of Biosciences最新文献_第7页

Floristic properties of lowland meadows in Central Black Sea Region of Turkey 土耳其黑海中部低地草甸的植物区系特征

Eurasian Journal of Biosciences Pub Date : 2011-07-01 DOI: 10.5053/EJOBIOS.2011.5.0.7

E. Yalçın, M. Kilinç, H. Kutbay, A. Bilgin, H. Korkmaz

引用次数: 10

The application of amplified TSPY and amelogenin genes from maternal plasma as a non-invasive bovine fetal DNA diagnosis 从母体血浆中扩增TSPY和淀粉原蛋白基因作为无创牛胎儿DNA诊断的应用

Eurasian Journal of Biosciences Pub Date : 2011-07-01 DOI: 10.5053/EJOBIOS.2011.5.0.14

A. Davoudi, R. Seighalani, S. Aleyasin, A. Tarang, R. Radjabi, Farideh Tahmoressi

{"title":"The application of amplified TSPY and amelogenin genes from maternal plasma as a non-invasive bovine fetal DNA diagnosis","authors":"A. Davoudi, R. Seighalani, S. Aleyasin, A. Tarang, R. Radjabi, Farideh Tahmoressi","doi":"10.5053/EJOBIOS.2011.5.0.14","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2011.5.0.14","url":null,"abstract":"Some studies showed analysis of fetal DNA in maternal plasma had been introduced as a new method for non-invasive prenatal diagnosis. Fetal sexing is possible at 8th week of pregnancy, using maternal blood sample testing. The aim was providing a rapid, reliable and non-invasive \u0000method for sexing of bovine fetuses. Maternal blood samples were collected from 38 pregnant cows during the 8th-38th w of gestation. Plasma was obtained by centrifugation and DNA was extracted by phenol-chloroform method from 350 􀁺L maternal plasma. Two primer pairs for bovine amelogenin, Y-encoded, and testis-specific protein gene were used to amplification three fragments; 260 bp (Y-encoded, testis-specific protein gene), 341 and 467 bp (Y and X chromosome amelogenin gene). The polymerase chain reaction has been optimized for fragments amplification. The 467 bp fragment was detected in all samples. The 341 and 260 bp fragments were detected in 24 of 38 plasma samples of cows with male fetuses. The sensitivity and specificity of test was 100% with no false negative and positive results. The results showed that phenol-chloroform method was a simple and sensational to isolation fetal DNA in maternal plasma. The polymerase chain reaction is a favorable noninvasive method for bovine fetal sexing.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":"5 1","pages":"119-126"},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2011.5.0.14","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70598835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Embryonic and larval development of critically endangered riverine catfish Rita rita 极度濒危河流鲶鱼丽塔丽塔的胚胎和幼虫发育

Eurasian Journal of Biosciences Pub Date : 2011-07-01 DOI: 10.5053/EJOBIOS.2011.5.0.13

M. Mollah, K. Taslima, H. Rashid, Z. Hossain, M. Sarowar, Muhammad Rakibul Kabir Khan

引用次数: 11

Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15 蜡样芽孢杆菌CA15产胞外蛋白酶的最佳条件研究

Eurasian Journal of Biosciences Pub Date : 2011-01-01 DOI: 10.5053/EJOBIOS.2011.5.0.1

F. Uyar, Ilknur Porsuk, G. Kızıl, E. Yilmaz

引用次数: 86

Effects of 24-epibrassinolide on growth, chlorophyll, electrolyte leakage and proline by pepper plants under NaCl-stress nacl胁迫下24-表油菜素内酯对辣椒生长、叶绿素、电解质泄漏和脯氨酸的影响

Eurasian Journal of Biosciences Pub Date : 2010-07-01 DOI: 10.5053/EJOBIOS.2010.4.0.12

S. I. M. Houimli, M. Denden, Bouthaina Dridi Mouhandes

{"title":"Effects of 24-epibrassinolide on growth, chlorophyll, electrolyte leakage and proline by pepper plants under NaCl-stress","authors":"S. I. M. Houimli, M. Denden, Bouthaina Dridi Mouhandes","doi":"10.5053/EJOBIOS.2010.4.0.12","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2010.4.0.12","url":null,"abstract":"Brassinosteroids are steroidal phytohormones that have the ability to overcome plant environmental stress. This study was carried out to investigate the role of 24-epibrassinolide in inducing pepper plant salt tolerance as measured by a range of physiological parameters: growth, chlorophyll, electrolyte leakage and proline. Capsicum annuum cv. Beldi seedlings were sprayed with 24-epibrassinolide both in the presence or the absence of NaCl and were sampled, 28 days after treatments. As a result of analysing the cultures under salinity stress, it was determined that the biomass and the chlorophyll decreased significantly, while the electrolyte leakage and the proline concentration increased considerably under salinity stress. However, the application of 24-epibrassinolide significantly ameliorated the adverse effects of salinity on the examined parameters, confirming the suppositions of previous authors who have claimed that exogenously applied 24-epibrassinolide can increase growth and protect the integrity of the cellular membrane in stressed plants.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":"4 1","pages":"96-104"},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2010.4.0.12","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70597504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 97

A novel set of highly polymorphic chloroplast microsatellite and ISSR markers for the biofuel crop Jatropha curcas 生物燃料作物麻疯树叶绿体微卫星和ISSR标记的研究

Eurasian Journal of Biosciences Pub Date : 2010-07-01 DOI: 10.5053/EJOBIOS.2010.4.0.15

N. Mittal, A. Dubey

{"title":"A novel set of highly polymorphic chloroplast microsatellite and ISSR markers for the biofuel crop Jatropha curcas","authors":"N. Mittal, A. Dubey","doi":"10.5053/EJOBIOS.2010.4.0.15","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2010.4.0.15","url":null,"abstract":"A novel set of polymorphic ISSR and chloroplast microsatellite markers were identified to mark the genetic relationship among different accessions of Jatropha curcas. A total of 12 polymorphic ISSR primers used, amplified a total of 26 fragments among ten different accessions of J. curcas. The cross-species amplification of microsatellite loci is timesaving as well as a cost-effective approach for developing locus specific markers for new species. Further, in an attempt to reveal a high degree of polymorphism and genetic variation among different accessions of J. curcas chloroplast microsatellite primer pairs developed for Acorus calamus (Acoraceace) were examined for cross-species amplification and validation among them. Out of 18 cpSSRs, six (33.33%) were identified as resulting in good amplifications and polymorphism. The number of amplified alleles per cpSSR primer pair ranged from 2 to 4 with the total number of 18 size variants as whole. The 18 size variants at 6 loci among 10 accessions were combined in 4 different haplotypes. The total chloroplast DNA diversity (HT) and the differentiation (GST) were found as 0.700 and 0.560 respectively. Whereas, Shannon's information index (I), Nei's genetic diversity among different accessions of J. curcas using ISSR and cpSSR markers was estimated at 0.33±0.28; 0.22±0.19 and 0.94±0.35; 0.55±0.18 respectively. The genetic relationship among the accessions was established using UPGMA cluster analysis, which were grouped into two major clusters denoted as cluster-I and cluster-II. The implication of the results in devising the importance of a identified novel set of markers for the genetic relationship and genetic diversity analysis in J. curcas is discussed.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":"4 1","pages":"119-131"},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2010.4.0.15","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70597977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Colonization of tomato roots by some potentially human-pathogenic bacteria and their plant-beneficial properties 一些潜在的人类致病菌在番茄根部的定植及其对植物有益的特性

Eurasian Journal of Biosciences Pub Date : 2010-07-01 DOI: 10.5053/EJOBIOS.2010.4.0.14

D. Egamberdieva

{"title":"Colonization of tomato roots by some potentially human-pathogenic bacteria and their plant-beneficial properties","authors":"D. Egamberdieva","doi":"10.5053/EJOBIOS.2010.4.0.14","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2010.4.0.14","url":null,"abstract":"Many studies provide evidence for the occurrence of opportunistic pathogens in the rhizosphere of plants. The presence of a competitive microflora in soil may restrict root colonization by potentially pathogenic bacteria. The aim of the present work was to study the competitive tomato root tip colonization of potentially human pathogenic bacteria such as Bacillus cereus, Staphylacoccus saprophyticus, Pseudomonas aeruginosa, Acinetobacter sp. against best known root tip colonizer P. fluorescens WCS365 and to characterize for their ability to stimulate plant growth and to suppress tomato foot and root rot disease caused by Fusarium oxysporum f.sp. radicis-lycopersici. In competitive tomato root tip colonization assays all tested bacterial strains showed poor colonization of the tomato rhizosphere except P. aeruginosa which was better competitive colonizer than reference strains. All five bacterial strains stimulated shoot length (up to 35%) and dry matter (up to 58%) of tomato and only one strain B. cereus TSAU80 showed statistically significant disease control (27%) of tomato foot and root rot in comparison to the Fusarium-infected control plants (47%). These results show that the opportunistic pathogens possess beneficial effect on plant growth and are able to colonize the rhizosphere of tomato, potentially resulting risk of contamination for tomato greenhouses. However, their competitive colonization abilities in the rhizosphere are poor, whereas only P. aeruginosa appeared to be better competitive colonizer than reference strain.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":"4 1","pages":"112-118"},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2010.4.0.14","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70597758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Plant growth promoting activities of phosphate- solubilizing Enterobacter asburiae as influenced by fungicides 杀菌剂对溶磷型沙土肠杆菌促植物生长活性的影响

Eurasian Journal of Biosciences Pub Date : 2010-07-01 DOI: 10.5053/EJOBIOS.2010.4.0.11

M. Ahemad, M. S. Khan

{"title":"Plant growth promoting activities of phosphate- solubilizing Enterobacter asburiae as influenced by fungicides","authors":"M. Ahemad, M. S. Khan","doi":"10.5053/EJOBIOS.2010.4.0.11","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2010.4.0.11","url":null,"abstract":"Four fungicides, tebuconazole, hexaconazole, metalaxyl and kitazin were applied at recommended and higher rates to examine their effects on the plant growth promoting activities of the fungicide tolerant Enterobacter asburiae strain PS2 under in vitro conditions. Enterobacter asburiae strain PS2 was isolated from the mustard rhizosphere and was assessed for the fungicide-tolerance and production of plant growth promoting traits (phosphate solubilization, siderophores, indole acetic acid, exo-polysaccharides, hydrogen cyanide and ammonia production) both in the presence and absence of fungicides. Enterobacter asburiae strain PS2 showed plant growth promoting activities even in the presence of fungicides which however, decreased progressively with the increase in fungicide concentration. Fungicides at recommended dose had little effect while the dose higher than the recommended one adversely affected the physiological traits, like, phosphate solubilization, siderophore, and indole acetic acid synthesis. Among all fungicides, tebuconazole in general, showed maximum toxicity to plant growth promoting activities. Fungicide-tolerant E. asburiae strain PS2 with the inherent ability to produce growth regulators even in the presence of fungicides can be exploited as a bio-inoculant to increase the productivity of crops grown in fungicide stressed soils.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":"4 1","pages":"88-95"},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2010.4.0.11","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70597840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 83

Biological activities of extracts from Andaman Sea sponges, Thailand 泰国安达曼海海绵提取物的生物活性研究

Eurasian Journal of Biosciences Pub Date : 2010-07-01 DOI: 10.5053/EJOBIOS.2010.4.0.8

Patchara Pedpradab, Wanlapa Molex, V. Nukoolkarn, Udomsak Darumas

引用次数: 8

A morphological and anatomical investigation about two rare and endemic Crocus taxa (Iridaceae) from Southern Anatolia 标题南安纳托利亚两个罕见特有藏红花类群(鸢尾花科)的形态解剖研究

Eurasian Journal of Biosciences Pub Date : 2010-07-01 DOI: 10.5053/EJOBIOS.2010.4.0.7

N. Kandemir

{"title":"A morphological and anatomical investigation about two rare and endemic Crocus taxa (Iridaceae) from Southern Anatolia","authors":"N. Kandemir","doi":"10.5053/EJOBIOS.2010.4.0.7","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2010.4.0.7","url":null,"abstract":"The morphological and anatomical characteristics of Crocus reticulatus subsp. hittiticus and Crocus cancellatus subsp. lycius, which are under the danger of extinction, have been investigated. The distributions of the two endemic subspecies are limited in Turkey. The plant samples were collected from the South Anatolia Region. The subsp. hittiticus flowers in the early spring whereas subsp. lycius flowers in the autumn. Their corm tunics are coarsely reticulate-fibrous. The subsp. hittiticus has blackish anthers and purple striped outer segments. The subsp. lycius has flowers with very pale lilac coloured and veined. Also, the style of this subsp. is equal to their anthers. These morphological properties are characteristics for two the subspecies investigated. The transverse-sections of the root, stem and leaf parts of these subspecies have been examined and revealed. Their morphologic and anatomic structures are illustrated. The anatomic properties of the two subspecies have been determined to be similar to the anatomic properties of other species of the genus Crocus. Moreover, the crystal type on the organs of the subsp. lycius was investigated.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":"4 1","pages":"54-62"},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2010.4.0.7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70598161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13