Sequence and expression analysis of the spermatogenesis-specific gene cognates, wampa and Prosα6T, in Drosophila suzukii.

IF 1.3 4区 生物学 Q4 GENETICS & HEREDITY
Qinwen Xia, Kaleem Tariq, Daniel A Hahn, Alfred M Handler
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引用次数: 0

Abstract

The sterile insect technique (SIT) is a highly effective biologically-based method for the population suppression of highly invasive insect pests of medical and agricultural importance. The efficacy of SIT could be significantly enhanced, however, by improved methods of male sterilization that avoid the fitness costs of irradiation. An alternative sterilization method is possible by gene-editing that targets genes essential for sperm maturation and motility, rendering them nonfunctional, similar to the CRISPR-Cas9 targeting of β2-tubulin in the genetic model system, Drosophila melanogaster. However, since genetic strategies for sterility are susceptible to breakdown or resistance in mass-reared populations, alternative targets for sterility are important for redundancy or strain replacement. Here we have identified and characterized the sequence and transcriptional expression of two genes in a Florida strain of Drosophila suzukii, that are cognates of the D. melanogaster spermatocyte-specific genes wampa and Prosalpha6T. Wampa encodes a coiled-coil dynein subunit required for axonemal assembly, and the proteasome subunit gene, Prosalpha6T, is required for spermatid individualization and nuclear maturation. The reading frames of these genes differed from their NCBI database entries derived from a D. suzukii California strain by 44 and 8 nucleotide substitutions/polymorphisms, respectively, though all substitutions were synonymous resulting in identical peptide sequences. Expression of both genes is predominant in the male testis, and they share similar transcriptional profiles in adult males with β2-tubulin. Their amino acid sequences are highly conserved in dipteran species, including pest species subject to SIT control, supporting their potential use in targeted male sterilization strategies.

Abstract Image

苏氏果蝇精子发生特异性同源基因wampa和Prosα6T的序列与表达分析。
昆虫不育技术(SIT)是一种高效的基于生物的方法来抑制具有重要医学和农业意义的高入侵性害虫。然而,通过改进雄性绝育方法,避免辐照的适应度成本,可以显著增强SIT的功效。另一种灭菌方法是通过基因编辑,针对精子成熟和运动所必需的基因,使它们失去功能,类似于遗传模型系统果蝇中靶向β2-微管蛋白的CRISPR-Cas9。然而,由于不育的遗传策略在大规模饲养的种群中容易被破坏或抵抗,因此不育的替代目标对于冗余或品系替换很重要。在这里,我们已经鉴定和表征了两个基因的序列和转录表达在一个佛罗里达菌株的苏氏果蝇,这是同源的D.黑腹蛇精细胞特异性基因wampa和Prosalpha6T。Wampa编码轴突组装所需的线圈动力蛋白亚基,而蛋白酶体亚基基因Prosalpha6T是精细胞个体化和核成熟所必需的。这些基因的阅读框与来自加利福尼亚铃木氏菌的NCBI数据库条目分别存在44个和8个核苷酸替换/多态性差异,尽管所有替换都是同义的,导致相同的肽序列。这两个基因的表达在男性睾丸中占主导地位,它们在成年男性中与β2-微管蛋白有相似的转录谱。它们的氨基酸序列在双翅目物种中高度保守,包括受SIT控制的害虫物种,支持它们在有针对性的雄性绝育策略中的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genetica
Genetica 生物-遗传学
CiteScore
2.70
自引率
0.00%
发文量
32
审稿时长
>12 weeks
期刊介绍: Genetica publishes papers dealing with genetics, genomics, and evolution. Our journal covers novel advances in the fields of genomics, conservation genetics, genotype-phenotype interactions, evo-devo, population and quantitative genetics, and biodiversity. Genetica publishes original research articles addressing novel conceptual, experimental, and theoretical issues in these areas, whatever the taxon considered. Biomedical papers and papers on breeding animal and plant genetics are not within the scope of Genetica, unless framed in an evolutionary context. Recent advances in genetics, genomics and evolution are also published in thematic issues and synthesis papers published by experts in the field.
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