Toward Retinal Organoids in High-Throughput.

IF 7.8 2区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Stefan Erich Spirig, Magdalena Renner
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引用次数: 0

Abstract

Human retinal organoids recapitulate the cellular diversity, arrangement, gene expression, and functional aspects of the human retina. Protocols to generate human retinal organoids from pluripotent stem cells are typically labor intensive, include many manual handling steps, and the organoids need to be maintained for several months until they mature. To generate large numbers of human retinal organoids for therapy development and screening purposes, scaling up retinal organoid production, maintenance, and analysis is of utmost importance. In this review, we discuss strategies to increase the number of high-quality retinal organoids while reducing manual handling steps. We further review different approaches to analyze thousands of retinal organoids with currently available technologies and point to challenges that still await to be overcome both in culture and analysis of retinal organoids.

实现高通量视网膜有机体。
人类视网膜器官组织再现了人类视网膜的细胞多样性、排列、基因表达和功能方面。从多能干细胞生成人类视网膜器官组织的方法通常需要大量人力,包括许多人工处理步骤,而且器官组织需要维持数月直至成熟。要生成大量用于治疗开发和筛选的人类视网膜类器官,扩大视网膜类器官的生产、维护和分析规模至关重要。在这篇综述中,我们讨论了在减少人工处理步骤的同时增加高质量视网膜器官组织数量的策略。我们进一步回顾了利用现有技术分析数千个视网膜器官组织的不同方法,并指出了在视网膜器官组织的培养和分析方面仍有待克服的挑战。
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来源期刊
Cold Spring Harbor perspectives in medicine
Cold Spring Harbor perspectives in medicine MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
14.30
自引率
1.90%
发文量
44
审稿时长
4-8 weeks
期刊介绍: Cold Spring Harbor Perspectives in Medicine is a monthly online publication comprising reviews on different aspects of a variety of diseases, covering everything from the molecular and cellular bases of disease to translational medicine and new therapeutic strategies. Cold Spring Harbor Perspectives in Medicine is thus unmatched in its depth of coverage and represents an essential source where readers can find informed surveys and critical discussion of advances in molecular medicine.
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