Utility of BAP1, p16, and Methylthio-Adenosine Phosphorylase Immunohistochemistry in Cytological and Histological Samples of Pleural Mesotheliomas.

IF 1.6 4区 医学 Q3 PATHOLOGY
Acta Cytologica Pub Date : 2023-01-01 DOI:10.1159/000530002
Vera Amacher, Peter Karl Bode, Holger Moch, Daniela Lenggenhager, Bart Vrugt
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引用次数: 0

Abstract

Introduction: In most cases, the diagnostic workup of pleural mesotheliomas (MPMs) starts with cytological examination of pleural effusion, but histology is needed to confirm the diagnosis. The introduction of BAP1 and methylthio-adenosine phosphorylase (MTAP) immunohistochemistry has become a powerful tool to confirm the malignant nature of mesothelial proliferations also in cytological specimens. The objective of this study was to determine the concordance of BAP1, MTAP, and p16 expression between cytological and histological samples of patients with MPM.

Methods: Immunohistochemistry of BAP1, MTAP, and p16 was performed on cytological samples and compared with the corresponding histological specimen of 25 patients with MPM. Inflammatory and stromal cells served as positive internal control for all three markers. In addition, samples of 11 patients with reactive mesothelial proliferations served as an external control group.

Results: Loss of BAP1, MTAP, and p16 expression was found in 68%, 72%, and 92% of MPM, respectively. Loss of MTAP was associated with loss of p16 expression in all cases. Concordance of BAP1 between cytological and corresponding histological samples was 100% (kappa coefficient 1; p = 0.008). For MTAP and p16, kappa coefficient was 0.9 (p = 0.01) and 0.8 (p = 0.7788), respectively.

Conclusions: Concordant BAP1, MTAP, and p16 expression is found between cytological and corresponding histological samples, indicating that a reliable diagnosis of MPM can be made on cytology only. Of the three markers, BAP1 and MTAP are most reliable in distinguishing malignant from reactive mesothelial proliferations.

BAP1, p16和甲基硫代腺苷磷酸化酶免疫组化在胸膜间皮瘤细胞学和组织学样本中的应用。
简介:在大多数情况下,胸膜间皮瘤(MPMs)的诊断工作从胸膜积液细胞学检查开始,但需要组织学来确认诊断。引入BAP1和甲基硫代腺苷磷酸化酶(MTAP)免疫组化已成为细胞学标本中确认间皮增生恶性性质的有力工具。本研究的目的是确定MPM患者细胞学和组织学样本中BAP1、MTAP和p16表达的一致性。方法:对25例MPM患者细胞学标本进行BAP1、MTAP、p16的免疫组化,并与相应的组织学标本进行比较。炎症细胞和基质细胞是这三种标志物的阳性内控。此外,11例反应性间皮增生患者作为外部对照组。结果:BAP1、MTAP和p16的表达分别在68%、72%和92%的MPM中缺失。在所有病例中,MTAP的缺失都与p16表达的缺失相关。细胞学样本与相应的组织学样本BAP1的一致性为100% (kappa系数1;P = 0.008)。MTAP和p16的kappa系数分别为0.9 (p = 0.01)和0.8 (p = 0.7788)。结论:BAP1、MTAP、p16在细胞学和相应的组织学样本中表达一致,提示仅凭细胞学诊断可可靠诊断MPM。在这三种标志物中,BAP1和MTAP在区分恶性和反应性间皮细胞增殖方面是最可靠的。
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来源期刊
Acta Cytologica
Acta Cytologica 生物-病理学
CiteScore
3.70
自引率
11.10%
发文量
46
审稿时长
4-8 weeks
期刊介绍: With articles offering an excellent balance between clinical cytology and cytopathology, ''Acta Cytologica'' fosters the understanding of the pathogenetic mechanisms behind cytomorphology and thus facilitates the translation of frontline research into clinical practice. As the official journal of the International Academy of Cytology and affiliated to over 50 national cytology societies around the world, ''Acta Cytologica'' evaluates new and existing diagnostic applications of scientific advances as well as their clinical correlations. Original papers, review articles, meta-analyses, novel insights from clinical practice, and letters to the editor cover topics from diagnostic cytopathology, gynecologic and non-gynecologic cytopathology to fine needle aspiration, molecular techniques and their diagnostic applications. As the perfect reference for practical use, ''Acta Cytologica'' addresses a multidisciplinary audience practicing clinical cytopathology, cell biology, oncology, interventional radiology, otorhinolaryngology, gastroenterology, urology, pulmonology and preventive medicine.
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