Osteoprotective effects of kefir fortified with omega-3 and vitamin C in ovariectomized rats.

IF 2 4区 医学 Q3 NUTRITION & DIETETICS
Mahsa Moazen, Zohreh Mazloom, Nader Tanideh, Mohammad Hossein Dabbaghmanesh, Samane Rahmdel, Negar Azarpira, Mohammad Fararouei
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Abstract

Nutritional interventions can be valuable for the prevention of postmenopausal osteoporosis. This study aimed to investigate the effects of kefir fortified with omega-3 and vitamin C on the bone and uterus parameters of ovariectomized rats. Seventy-seven female Sprague-Dawley rats were ovariectomized or sham-operated. The ovariectomized rats were assigned to six groups and received 1 ml/day of distilled water (OVX group), milk, kefir, kefir fortified with omega-3 fatty acids (kefir+ω3), kefir fortified with vitamin C (kefir+vit-C) or kefir fortified with omega-3 and vitamin C (kefir+ω3+vit-C) for 12 weeks. The sham group also received 1ml/day of distilled water. Subsequently, bone mineral content (BMC) and bone mineral density (BMD) of various bones were assessed. Femurs and uteri were harvested for bone ash analysis and histopathological examinations, respectively. Sera were analyzed for carboxy-terminal cross-linked telopeptide of type 1 collagen, procollagen type 1 amino-terminal propeptide, calcium, phosphorous, tumor necrosis factor-α (TNF-α) and total antioxidant capacity levels. Ovariectomy resulted in significant reduction in bone density (P<0.05). Kefir+ω3+vit-C significantly improved BMC of lumbar spine (0.699±0.027 g compared with 0.580±0.018 in the OVX group), and kefir, kefir+vit-C and kefir+ω3+vit-C significantly increased BMD of tibia (0.118±0.003 g/cm2, 0.119±0.001 and 0.120±0.004 compared with 0.102±0.005 in the OVX group). Moreover, ovariectomy markedly elevated TNF-α level, which was significantly reversed by kefir+ω3+vit-C. Significant atrophy of the uterus was observed following ovariectomy, although the uterus parameters did not change by any of the interventions. In conclusion, kefir fortified with omega-3 fatty acids and vitamin C may have protective effects against bone loss through suppressing inflammation.

加omega-3和维生素C的开菲尔对去卵巢大鼠的骨保护作用。
营养干预对于预防绝经后骨质疏松症是有价值的。本研究旨在探讨加omega-3和维生素C的克菲尔对去卵巢大鼠骨骼和子宫参数的影响。77只雌性Sprague-Dawley大鼠被切除卵巢或假手术。将切除卵巢的大鼠分为6组,分别给予1 ml/d蒸馏水(OVX组)、牛奶、克非尔、加omega-3脂肪酸的克非尔(克非尔+ω3)、加维生素C的克非尔(克非尔+ω3+维生素C)或加omega-3和维生素C的克非尔(克非尔+ω3+维生素C),持续12周。假手术组同时给予蒸馏水1ml/d。随后,测定各骨的骨矿物质含量(BMC)和骨矿物质密度(BMD)。取股骨和子宫分别进行骨灰分析和组织病理学检查。分析血清中1型胶原羧基末端交联末端肽、前胶原1型氨基末端前肽、钙、磷、肿瘤坏死因子-α (TNF-α)及总抗氧化能力水平。卵巢切除术导致骨密度显著降低(P2, 0.119±0.001和0.120±0.004,而OVX组为0.102±0.005)。此外,卵巢切除术可显著提高TNF-α水平,而kefir+ω3+ vitc可显著逆转TNF-α水平。卵巢切除术后观察到子宫明显萎缩,尽管子宫参数没有因任何干预而改变。总之,加了omega-3脂肪酸和维生素C的克非尔可能通过抑制炎症对骨质流失有保护作用。
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来源期刊
CiteScore
5.00
自引率
4.30%
发文量
53
审稿时长
>12 weeks
期刊介绍: Since 1930 this journal has provided an important international forum for scientific advances in the study of nutrition and vitamins. Widely read by academicians as well as scientists working in major governmental and corporate laboratories throughout the world, this publication presents work dealing with basic as well as applied topics in the field of micronutrients, macronutrients, and non-nutrients such as secondary plant compounds. The editorial and advisory boards include many of the leading persons currently working in this area. The journal is of particular interest to: - Nutritionists - Vitaminologists - Biochemists - Physicians - Engineers of human and animal nutrition - Food scientists
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