[Consensus of clinical pathways of metagenomic next-generation sequencing test in diagnosis of lower respiratory tract infections in China].

Abstract

Metagenomic next-generation sequencing (mNGS) enables sensitive and unbiased detection of all potential pathogens in lower respiratory tract infection(LRTI). But the lack of consensus and uniformity of applicable clinical scenarios and interpretation of results for mNGS largely limit its utilization. To solve these issues, an expert panel including pulmonary physicians, intensivists and microbiologists is organized by Chinese Thoracic Society. The panel conducted a systematic review of progress and challenges regarding the application of mNGS on etiological diagnosis of LRTI. The panel formulated 17 specific recommendations spanning questions of suitable clinical situations, sample quality control, process of reports interpretation and subsequent medical decisions. Hopefully, this consensus can provide practical guidelines for clinicians and promote the rational application of mNGS in LRTI.