Performance of Immunohistochemical and Molecular Methods in Detecting Microsatellite Instability in Gastric Cancer: A Multicenter Study.

IF 3.5 4区医学 Q3 CELL BIOLOGY

Pathobiology Pub Date : 2023-01-01 Epub Date: 2023-06-02 DOI:10.1159/000530997

Diogo Sousa Marques, Irene Gullo, Luís Mascarenhas-Lemos, João Ricardo Silva, Catarina Neto do Nascimento, Patrícia Pontes, Lídia Pinho, Luis Cirnes, Xiaogang Wen, Marília Cravo, Fátima Carneiro

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引用次数: 1

Abstract

Introduction: Microsatellite instability (MSI) is an important prognostic molecular biomarker for gastric cancer (GC). MSI status may be detected by immunohistochemistry (IHC) for mismatch repair (MMR) proteins and polymerase chain reaction (PCR). Idylla™ MSI assay has not been validated for GC but may prove to be a valid alternative.

Methods: In a series of 140 GC cases, MSI status was evaluated by IHC for MLH1, PMS2, MSH2, and MSH6; gold-standard pentaplex PCR panel (PPP) (BAT-25, BAT-26, NR-21, NR-24, and NR-27); and Idylla. Statistical analysis was performed using SPSS 27.0.

Results: PPP identified 102 microsatellite stable (MSS) cases and 38 MSI-high cases. Only 3 cases showed discordant results. Compared with PPP, the sensitivity was 100% for IHC and 94.7% for Idylla. Specificity was 99% for IHC and 100% for Idylla. MLH1 IHC alone showed sensitivity and specificity of 97.4% and 98.0%, respectively. IHC identified three indeterminate cases; all were MSS according to PPP and Idylla.

Conclusion: IHC for MMR proteins represents an optimal screening tool for MSI status in GC. If resources are limited, isolated MLH1 evaluation may constitute a valuable option for preliminary screening. Idylla may help detect rare MSS cases with MMR-loss and define MSI status in indeterminate cases.

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检测胃癌微卫星不稳定性的免疫组化和分子方法的性能：一项多中心研究

简介微卫星不稳定性（MSI）是胃癌（GC）重要的预后分子生物标志物。MSI 状态可通过错配修复（MMR）蛋白的免疫组化（IHC）和聚合酶链反应（PCR）来检测。Idylla™ MSI 检测法尚未在 GC 中得到验证，但可能被证明是一种有效的替代方法：在一系列 140 例 GC 病例中，通过 IHC 检测 MLH1、PMS2、MSH2 和 MSH6；黄金标准五重 PCR 面板 (PPP)（BAT-25、BAT-26、NR-21、NR-24 和 NR-27）；以及 Idylla 评估 MSI 状态。使用 SPSS 27.0 进行统计分析：PPP确定了102个微卫星稳定（MSS）病例和38个MSI-高病例。只有 3 例结果不一致。与 PPP 相比，IHC 的灵敏度为 100%，Idylla 的灵敏度为 94.7%。IHC 的特异性为 99%，Idylla 为 100%。仅 MLH1 IHC 的敏感性和特异性分别为 97.4% 和 98.0%。IHC发现了三个不确定病例；根据PPP和Idylla，所有病例均为MSS：结论：MMR 蛋白的 IHC 是筛查 GC 中 MSI 状态的最佳工具。如果资源有限，分离 MLH1 评估可能是初步筛查的重要选择。Idylla可帮助发现MMR缺失的罕见MSS病例，并确定不确定病例的MSI状态。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathobiology 医学-病理学

CiteScore

8.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： ''Pathobiology'' offers a valuable platform for the publication of high-quality original research into the mechanisms underlying human disease. Aiming to serve as a bridge between basic biomedical research and clinical medicine, the journal welcomes articles from scientific areas such as pathology, oncology, anatomy, virology, internal medicine, surgery, cell and molecular biology, and immunology. Published bimonthly, ''Pathobiology'' features original research papers and reviews on translational research. The journal offers the possibility to publish proceedings of meetings dedicated to one particular topic.