Involvement of glyphosate in disruption of biotransformation P450 enzymes and hepatic lipid metabolism in chicken.

IF 1.7 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-05-21 DOI:10.1080/10495398.2023.2214601

Mohamed Ahmed Fathi, Shen Dan, Adel Mohamed Abdelsalam, Li Chunmei

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引用次数: 0

Abstract

The current study investigated the potentially harmful consequences of pure glyphosate or Roundup® on CYP family members and lipid metabolism in newly hatched chicks. On the sixth day, 225 fertilized eggs were randomly divided into three treatments: (1) the control group injected with deionized water, (2) the glyphosate group injected with 10 mg pure glyphosate/Kg egg mass and (3) the Roundup group injected 10 mg the active ingredient glyphosate in Roundup®/Kg egg. The results of the study revealed a reduction in hatchability in chicks treated with Roundup^®. Moreover, change of Lipid concentration in serum and the liver-treated groups. Additionally, increased liver function enzymes and increased oxidative stress in the glyphosate and Roundup^® groups. Furthermore, liver tissues showed histological changes and several lipid deposits in glyphosate-treated groups. Hepatic CYP1A2 and CYP1A4 expressions were significantly increased (p < .05) after glyphosate exposure, and suppression of CYP1C1 mRNA expression was significant (p < .05) after Roundup^® exposure. The pro-inflammatory cytokines genes IFN-γ and IL-1β expression were significantly increased (p < .05) after Roundup^® exposure. In addition, there were significant differences in the levels of expression genes which are related to lipid synthesis or catabolism in the liver. In conclusion, in ovo glyphosate exposure caused disruption of biotransformation, pro-inflammatory and lipid metabolism in chicks.

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草甘膦参与破坏鸡体内的生物转化 P450 酶和肝脏脂质代谢。

本研究调查了纯草甘膦或 Roundup® 对刚孵化的雏鸡的 CYP 家族成员和脂质代谢的潜在有害影响。第六天，将 225 枚受精卵随机分为三个处理：(1) 注射去离子水的对照组；(2) 注射 10 毫克纯草甘膦/千克鸡蛋的草甘膦组；(3) 注射 10 毫克 Roundup® 中草甘膦活性成分/千克鸡蛋的 Roundup 组。研究结果表明，使用 Roundup® 处理的雏鸡孵化率降低。此外，血清和肝脏处理组的血脂浓度发生变化。此外，草甘膦组和 Roundup® 组的肝功能酶增加，氧化应激增加。此外，草甘膦处理组的肝组织显示出组织学变化和一些脂质沉积。肝脏 CYP1A2 和 CYP1A4 表达量显著增加（p p ® 暴露）。促炎细胞因子基因 IFN-γ 和 IL-1β 的表达明显增加（p ® 暴露）。此外，与肝脏中脂质合成或分解有关的基因的表达水平也存在明显差异。总之，草甘膦的卵内暴露会破坏雏鸡的生物转化、促炎和脂质代谢。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animal Biotechnology 工程技术-奶制品与动物科学

CiteScore

2.90

自引率

5.40%

发文量

230

审稿时长

>12 weeks

期刊介绍： Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology. Submissions on the following topics are particularly welcome: - Applied microbiology, immunogenetics and antibiotic resistance - Genome engineering and animal models - Comparative genomics - Gene editing and CRISPRs - Reproductive biotechnologies - Synthetic biology and design of new genomes