hsa_circ_0051428 Facilitates the Progression of Thyroid Cancer by Sponging miR-1248 to Up-Regulate FN1.

IF 1.5 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Tao Zhou, Yuanyuan Zhang, Shilin Zheng, Fuhua Wang, Shengpan Jiang, Wenfeng Lei, Lili Xu, Yiqing Tan
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引用次数: 0

Abstract

Evidence displays that circular RNAs (circRNAs) are considerable mediators of numerous processes in cancer development. Given that many circRNAs are not functionally characterized, our aim was to explore the function and mechanisms of circ_0051428 in thyroid cancer (TC). The analysis of circ_0051428, miR-1248 and FN1 mRNA expression was conducted using real-time quantitative polymerase chain reaction. Cell growth was observed using CCK-8 and colony formation assays. Cell migration was investigated using wound healing assay. Cell apoptosis was identified by the expression of apoptosis-related proteins (Bax and Bcl-2) using Western blotting. Animal models were established to testify the role of circ_0051428 in vivo. The assumed binding between miR-1248 and circ_0051428 or FN1 was identified using dual-luciferase reporter or RIP assay. circ_0051428 exhibits an abnormally elevated expression in TC. circ_0051428 deficiency caused inhibition of TC cell proliferation, migration, clonogenic capacity, and inhibition of tumor growth in vivo. circ_0051428 directly targeted miR-1248, and FN1 was a target downstream of circ_0051428/miR-1248 axis. circ_0051428 could sponge miR-1248 to upregulate FN1. Furthermore, miR-1248 downregulation recovered circ_0051428 deficiency-suppressed cancer cell proliferation, survival and migration. Besides, the repressive effects of FN1 knockdown on cancer cell growth, survival and migration were also partly abolished by miR-1248 downregulation. circ_0051428 targeted miR-1248 to modulate FN1 expression, thereby facilitating the malignant progression of TC, which contributed to the understanding of the molecular mechanism of TC development.

hsa_circ_0051428通过海绵miR-1248上调FN1促进甲状腺癌的进展。
有证据表明,环状rna (circRNAs)在癌症发展的许多过程中是相当重要的介质。鉴于许多circrna没有功能特征,我们的目的是探索circ_0051428在甲状腺癌(TC)中的功能和机制。采用实时定量聚合酶链反应分析circ_0051428、miR-1248和FN1 mRNA的表达。用CCK-8和菌落形成法观察细胞生长情况。采用伤口愈合实验研究细胞迁移。Western blotting检测细胞凋亡相关蛋白Bax和Bcl-2的表达。建立动物模型验证circ_0051428在体内的作用。假设miR-1248与circ_0051428或FN1之间的结合是通过双荧光素酶报告基因或RIP测定来确定的。circ_0051428在TC中表达异常升高。circ_0051428缺失导致体内TC细胞增殖、迁移、克隆生成能力受到抑制,肿瘤生长受到抑制。circ_0051428直接靶向miR-1248,而FN1是circ_0051428/miR-1248轴下游的靶标。circ_0051428可以海绵miR-1248上调FN1。此外,miR-1248下调恢复了circ_0051428缺陷,抑制了癌细胞的增殖、存活和迁移。此外,FN1敲低对癌细胞生长、存活和迁移的抑制作用也被miR-1248下调部分消除。circ_0051428靶向miR-1248调控FN1的表达,从而促进TC的恶性进展,有助于了解TC发生的分子机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Critical Reviews in Eukaryotic Gene Expression
Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物
CiteScore
2.70
自引率
0.00%
发文量
67
审稿时长
1 months
期刊介绍: Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.
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