Sonication as a tool for disrupting biofilms and recovering microorganisms in bladder catheters.

IF 1.3 Q3 UROLOGY & NEPHROLOGY

Jornal brasileiro de nefrologia : 'orgao oficial de Sociedades Brasileira e Latino-Americana de Nefrologia Pub Date : 2023-07-01 DOI:10.1590/2175-8239-JBN-2022-0129en

Juliette Cieslinski, Victoria Stadler Tasca Ribeiro, Camila Kowodzeichak de Lima, Letícia Kraft, Paula Hansen Suss, Felipe Francisco Tuon

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引用次数: 0

Abstract

Introduction: Urinary catheter-related infection is commonly associated with bacterial biofilm. The impact of anaerobes is unknown, but their detection in the biofilm on this device has not been previously reported. This study aimed to evaluate the capability to recovery strict, facultative, and aerobic microorganisms in patients using bladder catheters from ICUs using conventional culture, sonication, urinary analysis, and mass spectrometry.

Methods: Parallel, sonicated bladder catheters from 29 critically ill patients were compared with their routine urine culture. Identification was performed using matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry.

Results: The positivity rate in urine (n = 2, 3.4%) was lower than that in sonicated catheters (n = 7, 13.8%).

Conclusion: Bladder catheter sonication showed more positive culture results than urine samples for anaerobic and aerobic microorganisms. The role of anaerobes in urinary tract infection and catheter biofilm is discussed.

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超声作为破坏膀胱导尿管生物膜和恢复微生物的工具。

导读:导尿管相关性感染通常与细菌生物膜有关。厌氧菌的影响尚不清楚，但在该装置的生物膜中检测到厌氧菌尚无报道。本研究旨在通过常规培养、超声、尿液分析和质谱分析，评估使用icu膀胱导管的患者恢复严格、兼性和需氧微生物的能力。方法:对29例危重患者行平行超声膀胱导尿管尿常规培养进行比较。采用基质辅助激光解吸/电离飞行时间质谱法进行鉴定。结果:尿尿阳性率(n = 2, 3.4%)低于超声导尿管阳性率(n = 7, 13.8%)。结论:膀胱导管超声对厌氧和好氧微生物的培养结果高于尿样。讨论了厌氧菌在尿路感染和尿路生物膜中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Jornal brasileiro de nefrologia : 'orgao oficial de Sociedades Brasileira e Latino-Americana de Nefrologia Medicine-Medicine (all)

CiteScore

2.20

自引率

16.70%

发文量

208

审稿时长

16 weeks