Activity of meropenem-vaborbactam against different beta-lactamase producing Klebsiella pneumoniae and Escherichia coli isolates in Iran.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Samira Amereh, Fatemeh Zeynali Kelishomi, Fatemeh Ghayaz, Amir Javadi, Amir Peymani, Fatemeh Fardsanei, Ehsan Aali, Farhad Nikkhahi
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引用次数: 0

Abstract

We evaluated the activity of meropenem-vaborbactam against different beta-lactamase producing Klebsiella pneumoniae and Escherichia coli isolates. In our study antibiotic susceptibility testing, double disk synergy test, modified Hodge test were applied. Detection of ESBL, AmpC, and carbapenemase genes was performed by PCR. Multilocus sequence typing (MLST) analysis was done on OXA-48 producing K. pneumoniae strains. Our results showed that among E. coli and K. pneumoniae isolates, 41.1% and 40% of strains produced ESBL, respectively. Additionally, the prevalence of AmpC producing K. pneumoniae and E. coli was 4% and 45.5%, respectively. Altogether 64.2% of K. pneumoniae strains and one E. coli isolate produced carbapenemase. Among OXA-48 producing K. pneumoniae strains ST3500 and ST2528 were detected by MLST. Based on the phenotypic results of this study, vaborbactam was an effective inhibitor on the third-generation cephalosporin-resistant isolates (P < 0.0001). Meropenem-vaborbactam combination had the highest efficacy on KPC producing strains, and it had limited activity on isolates producing OXA-48 type beta-lactamases, whereas no effect was observed on NDM-1 producing isolates. Our study provided valuable information regarding the vaborbactam inhibitory effect on β-lactamase-producing strains.

美罗培尼-瓦波巴坦对伊朗产β -内酰胺酶的肺炎克雷伯菌和大肠杆菌的活性研究
我们评估了美罗培尼-瓦波巴坦对不同产β -内酰胺酶肺炎克雷伯菌和大肠杆菌分离株的活性。本研究采用药敏试验、双盘协同试验、改良霍奇试验。PCR检测ESBL、AmpC和碳青霉烯酶基因。对产OXA-48肺炎克雷伯菌进行多位点序列分型分析。结果表明,大肠杆菌和肺炎克雷伯菌分离株中产生ESBL的比例分别为41.1%和40%。此外,产AmpC的肺炎克雷伯菌和大肠杆菌的患病率分别为4%和45.5%。64.2%的肺炎克雷伯菌和1株大肠杆菌分离株产生碳青霉烯酶。在产OXA-48的肺炎克雷伯菌中,MLST检测到ST3500和ST2528菌株。根据本研究的表型结果,vaborbactam是第三代头孢菌素耐药菌株的有效抑制剂(P < 0.0001)。美罗培宁-瓦波巴坦联合用药对产KPC菌株的效果最高,对产OXA-48型β -内酰胺酶的菌株活性有限,而对产NDM-1的菌株无影响。我们的研究为瓦波巴坦对β-内酰胺酶产生菌的抑制作用提供了有价值的信息。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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