Chemical Components Analyzed by UPLC-QTOF-MS and In Vitro Antitumor Effects of the Chestnut Mushroom Pholiota adiposa (Agaricomycetes).

IF 1.4 4区 生物学 Q4 MYCOLOGY
Xiaoyan Wang, Yan Liu, Fanli Kong, Gang Li
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引用次数: 0

Abstract

We investigated the chemical constituents and anti-tumor activity of cultivated Pholiota adiposa in vitro using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry. HepG-2, A549, HeLa, and MCF-7 cells, which are 4 kinds of human cancer cell lines, were cultured in vitro, treated with different concentrations of the ethanol extract of Ph. adiposa (EPA), and cytotoxicity was determined using the cell counting kit-8 assay. Flow cytometry was used to analyze the apoptosis of HepG-2 cells via annexin V-fluorescein isothiocyanate/propidium iodide double staining. Expression levels of apoptosis-associated proteins were determined via Western blotting analysis. Thirty-five components were consistent with those recorded in the chemical composition database, with sterols, fatty acids, and polysaccharide compounds accounting for a relatively high proportion. EPA showed the strongest cytotoxicity against HepG-2 cells, increasing the apoptosis rate up to 23.71 ± 1.59% at a concentration of 50 μg/mL. Ph. adiposa has various functional chemical constituents and potential anti-tumor applications. We found that the functional constituents exerted anti-tumor activity by inducing apoptosis. Furthermore, the expression levels of BCL-2-associated X were increased, whereas those of BCL-2 were decreased in cells after treatment with EPA. These results suggest that EPA induces HepG-2 cell apoptosis via a caspase-mediated pathway.

UPLC-QTOF-MS分析板栗菌脂生菌的化学成分及体外抗肿瘤作用
采用超高效液相色谱-四极杆飞行时间质谱法研究了体外培养脂肪藻的化学成分和抗肿瘤活性。体外培养4种人癌细胞系HepG-2、A549、HeLa和MCF-7细胞,用不同浓度的Ph. adiposa乙醇提取物(EPA)处理,采用细胞计数试剂盒-8法测定细胞毒性。流式细胞术采用膜联蛋白v -异硫氰酸荧光素/碘化丙啶双染色法分析HepG-2细胞的凋亡情况。凋亡相关蛋白的表达水平通过Western blotting分析。35种成分与化学成分数据库中记录的一致,其中甾醇、脂肪酸和多糖化合物所占比例较高。EPA对HepG-2细胞的细胞毒性最强,在浓度为50 μg/mL时,其细胞凋亡率可达23.71±1.59%。脂肪脂具有多种功能化学成分和潜在的抗肿瘤应用。我们发现功能成分通过诱导细胞凋亡发挥抗肿瘤活性。此外,EPA处理后细胞中BCL-2相关X的表达水平升高,而BCL-2的表达水平降低。这些结果表明EPA通过caspase介导的途径诱导HepG-2细胞凋亡。
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来源期刊
CiteScore
2.60
自引率
16.70%
发文量
91
审稿时长
6-12 weeks
期刊介绍: The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.
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