TLN1 synergizes with ITGA5 to ameliorate cardiac microvascular endothelial cell dysfunction.

IF 1.2 4区医学 Q3 ANATOMY & MORPHOLOGY

Folia morphologica Pub Date : 2024-01-01 Epub Date: 2023-05-05 DOI:10.5603/FM.a2023.0031

Xianfeng Wang, Wenkai Mao, Xiaofeng Ma

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引用次数: 0

Abstract

Background: The complex process of atherosclerosis is thought to begin with endothelial cell dysfunction, and advanced atherosclerosis is the underlying cause of coronary artery disease (CAD). Uncovering the underlying mechanisms of CAD-related endothelial cell injury may contribute to the treatment.

Materials and methods: Cardiac microvascular endothelial cells (CMVECs) were treated with oxidised low-density lipoprotein (ox-LDL) to mimic an injury model. The involvement of Talin-1 (TLN1) and integrin alpha 5 (ITGA5) in the proliferation, apoptosis, angiogenesis, inflammatory response, and oxidative stress in CMVECs were assessed.

Results: TLN1 overexpression assisted CMVECs in resistance to ox-LDL stimulation, with alleviated cell proliferation and angiogenesis, reduced apoptosis, inflammatory response, and oxidative stress. TLN1 overexpression triggered increased ITGA5, and ITGA5 knockdown reversed the effects of TLN1 overexpression on the abovementioned aspects. Together, TLN1 synergized with ITGA5 to ameliorate the dysfunction in CMVECs.

Conclusions: This finding suggests their probable involvement in CAD, and increasing their levels is beneficial to disease relief.

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TLN1 与 ITGA5 协同改善心脏微血管内皮细胞功能障碍。

背景：动脉粥样硬化的复杂过程被认为始于内皮细胞功能障碍，晚期动脉粥样硬化是冠状动脉疾病（CAD）的根本原因。揭示与冠状动脉粥样硬化相关的内皮细胞损伤的潜在机制可能有助于治疗：用氧化低密度脂蛋白（ox-LDL）处理心脏微血管内皮细胞（CMVECs）以模拟损伤模型。评估了Talin-1（TLN1）和整合素α5（ITGA5）参与CMVECs增殖、凋亡、血管生成、炎症反应和氧化应激的情况：结果：TLN1的过表达有助于CMVECs抵抗ox-LDL刺激，缓解细胞增殖和血管生成，减少细胞凋亡、炎症反应和氧化应激。TLN1 过表达会引发 ITGA5 的增加，而 ITGA5 的敲除会逆转 TLN1 过表达对上述方面的影响。总之，TLN1与ITGA5协同改善了CMVECs的功能障碍：结论：这一发现表明它们可能参与了冠状动脉粥样硬化，提高它们的水平有利于缓解疾病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Folia morphologica ANATOMY & MORPHOLOGY-

CiteScore

2.40

自引率

0.00%

发文量

218

审稿时长

6-12 weeks

期刊介绍： "Folia Morphologica" is an official journal of the Polish Anatomical Society (a Constituent Member of European Federation for Experimental Morphology - EFEM). It contains original articles and reviews on morphology in the broadest sense (descriptive, experimental, and methodological). Papers dealing with practical application of morphological research to clinical problems may also be considered. Full-length papers as well as short research notes can be submitted. Descriptive papers dealing with non-mammals, cannot be accepted for publication with some exception.