Effect of simvastatin on osteogenesis of the extremity bones in aging rats.

IF 2.8 4区医学 Q3 CELL BIOLOGY

Connective Tissue Research Pub Date : 2023-01-01 DOI:10.1080/03008207.2022.2094790

Mengran Wang, Haowei Li, Jiaxin Tang, Yue Xi, Shiyi Chen, Ming Liu

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引用次数: 0

Abstract

Purpose: Simvastatin is a prodrug of the potent 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor. The main purpose of the current study is to assess the accurate function of simvastatin on osteoporosis of extremity bones in aging rats.

Materials and methods: Fifty 15-month-old SD rats were divided into five groups (four simvastatin groups and one control group). The rats in four simvastatin groups were fed with different doses of simvastatin (5, 10, 20, and 40 mg/kg/d, respectively) for 3 months, whereas the rats in control group were fed the equal physiological saline. Calcium (Ca), phosphorus (P), and the lipid spectrum in serum were measured. Biochemical markers of bone metabolism, osteocalcin (OC), and tartrate-resistant acid phosphatase (Trap-5b), were analyzed using ELISA. The content of adipocytes in bone marrow was analyzed by histological staining. Finally, the bone quality of the femur and tibia were evaluated using dual-energy X-ray absorptiometry (DEXA), peri-quantity CT (pQCT), and the 3-point bending biomechanical test.

Results: Simvastatin reduced serum triglycerides (TG), and 10 mg/kg/d of simvastatin significantly reduced the content of adipocytes in bone marrow compared to the control group. However, statistically significant differences between the simvastatin groups and the control group were not found in the CA, P, OC, Trap-5b, or the evaluation indexes of bone quality from DEXA, pQCT, and biomechanical tests.

Conclusion: Simvastatin could not prevent osteoporosis of the extremity bones in aging rats.

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辛伐他汀对衰老大鼠四肢骨成骨的影响。

目的:辛伐他汀是一种有效的3-羟基-3-甲基戊二酰辅酶a还原酶抑制剂的前药。本研究的主要目的是评估辛伐他汀对衰老大鼠四肢骨骼骨质疏松症的准确作用。材料与方法:将50只15月龄SD大鼠分为5组(4个辛伐他汀组和1个对照组)。4个辛伐他汀组分别饲喂不同剂量辛伐他汀(5、10、20、40 mg/kg/d) 3个月，对照组饲喂等量生理盐水。测定血清钙(Ca)、磷(P)和血脂谱。采用ELISA法分析骨代谢生化指标骨钙素(OC)和抗酒石酸酸性磷酸酶(Trap-5b)。采用组织学染色法分析骨髓脂肪细胞含量。最后，采用双能x线吸收仪(DEXA)、围量CT (pQCT)和三点弯曲生物力学试验评估股骨和胫骨的骨质量。结果:辛伐他汀降低血清甘油三酯(TG)，与对照组相比，10 mg/kg/d辛伐他汀显著降低骨髓脂肪细胞含量。然而，辛伐他汀组与对照组在CA、P、OC、Trap-5b以及DEXA、pQCT和生物力学试验中骨质量评价指标均无统计学差异。结论:辛伐他汀对老年大鼠四肢骨质疏松无预防作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Connective Tissue Research 生物-细胞生物学

CiteScore

6.60

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： The aim of Connective Tissue Research is to present original and significant research in all basic areas of connective tissue and matrix biology. The journal also provides topical reviews and, on occasion, the proceedings of conferences in areas of special interest at which original work is presented. The journal supports an interdisciplinary approach; we present a variety of perspectives from different disciplines, including Biochemistry Cell and Molecular Biology Immunology Structural Biology Biophysics Biomechanics Regenerative Medicine The interests of the Editorial Board are to understand, mechanistically, the structure-function relationships in connective tissue extracellular matrix, and its associated cells, through interpretation of sophisticated experimentation using state-of-the-art technologies that include molecular genetics, imaging, immunology, biomechanics and tissue engineering.