The possible protective role of vitamin C versus melatonin on potassium dichromate induced changes in rat thyroid gland: light and electron microscopic study.
Eman Saeed, Ahmed A El-Mansy, Shireen A Mazroa, Amal M Moustafa
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引用次数: 0
Abstract
Potassium dichromate is widely used in various laboratory and industrial applications. Vitamin C and melatonin are well-known antioxidants. Study the microscopic and morphometric alterations in the thyroid gland in adult male albino rats after the administration of potassium dichromate for successive 2 months and also to assess the possible protective effect of vitamin C versus melatonin on these changes. Sixty adult male albino rats were randomly divided into four main groups. Group I (The control group). Group II received potassium dichromate (25 mg/kg/day) dissolved in distilled water by intraperitoneal (i.p) injection for 2 months. Group III received the same dose of potassium dichromate with vitamin C (120 mg/kg/day) orally through an intragastric intubation. Group IV received the same dose of potassium dichromate and melatonin (10 mg/kg/day) as an i.p injection. Thyroid gland samples were prepared for light and electron microscopic studies. Potassium dichromate group demonstrated congested blood vessels, follicular hyperplasia, follicular enlargement with degenerated lining cells that were exfoliated in the lumen. The parafollicular cells appeared with darkly stained nuclei. PAS reaction showed weak reaction in the colloid with an abnormal pattern of vacuolization. A highly significant increase in the percentage area of fibrosis was detected in Mallory trichrome sections. Ultrastructurally, follicular cells and parafollicular cells appeared irregular in shape with dark, small heterochromatic nuclei. Small, electron-dense granules in the parafollicular cells were found. Potassium dichromate and vitamin C-treated group III showed partial improvement of the thyroid gland. The PAS reaction showed that nearly all the follicles were more or less similar to those of the control group. A significant decrease in the percentage area of fibrosis in group III was found as compared to those in group II. Potassium dichromate and melatonin-treated group showed that the thyroid gland was nearly similar to that of the control group. Vitamin C and melatonin could partially protect against potassium dichromate induced changes in the thyroid gland and the protective effect of melatonin was better than that of vitamin C.
重铬酸钾广泛应用于各种实验室和工业领域。维生素 C 和褪黑激素是著名的抗氧化剂。研究成年雄性白化大鼠连续两个月服用重铬酸钾后甲状腺的显微和形态变化，并评估维生素 C 和褪黑素对这些变化可能产生的保护作用。将 60 只成年雄性白化大鼠随机分为四大组。第一组（对照组）。第二组腹腔注射溶于蒸馏水的重铬酸钾（25 毫克/千克/天），为期 2 个月。第三组通过胃内插管口服相同剂量的重铬酸钾和维生素 C（120 毫克/千克/天）。第四组接受相同剂量的重铬酸钾和褪黑素（10 毫克/千克/天）静脉注射。制备甲状腺样本用于光镜和电子显微镜研究。重铬酸钾组表现为血管充血、滤泡增生、滤泡增大，内膜细胞变性并在管腔中脱落。卵泡旁细胞核呈深色染色。PAS 反应显示胶体反应微弱，空泡化形态异常。在马洛里三色染色切片中，发现纤维化的百分比面积明显增加。从超微结构上看，卵泡细胞和卵泡旁细胞的形状不规则，核色深且小，呈异色。在卵泡旁细胞中发现了电子致密的小颗粒。重铬酸钾和维生素 C 处理的 III 组甲状腺得到部分改善。PAS反应显示，几乎所有的滤泡都与对照组的滤泡大致相似。与第二组相比，第三组的纤维化面积百分比明显减少。重铬酸钾和褪黑激素治疗组的甲状腺几乎与对照组相似。维生素C和褪黑素对重铬酸钾引起的甲状腺变化有部分保护作用，且褪黑素的保护作用优于维生素C。
期刊介绍：
Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology.
Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics:
Advances in the uses of electron microscopic and immunohistochemical techniques
Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis
Important new, investigative, clinical, and diagnostic EM methods.
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