Juliete L. Viana , Joelma S. da Silva , Gabriela C. de Mattos , Martina C.C. Pinto , Luciana da S. Dutra , Larissa L. de A. Carvalho , José Carlos C. da S. Pinto , Valéria Cristina S. Pinheiro , Rosemary A. Roque
{"title":"Microencapsulation of Bacillus thuringiensis strains for the control of Aedes aegypti","authors":"Juliete L. Viana , Joelma S. da Silva , Gabriela C. de Mattos , Martina C.C. Pinto , Luciana da S. Dutra , Larissa L. de A. Carvalho , José Carlos C. da S. Pinto , Valéria Cristina S. Pinheiro , Rosemary A. Roque","doi":"10.1016/j.exppara.2023.108654","DOIUrl":null,"url":null,"abstract":"<div><p><span>In this study, we investigated the microencapsulation<span> of two strains of the entomopathogenic bacteria </span></span><span><em>Bacillus thuringiensis</em></span> (<em>B. thuringiensis</em>) (BtMA-750 and BtMA-1114), which are biopesticides of high toxicity for the mosquito vector <span><em>Aedes aegypti</em></span>. The encapsulation of different concentrations of microorganisms in starch microparticles was evaluated, and the inverse suspension polymerization technique was explored. It was possible to observe that the higher amounts of the biopesticide caused a slight decrease in the diameter of the particles; however, even when encapsulated, the biopesticide still presents an average diameter that is able to be consumed by the larvae of <em>Aedes aegypti</em>. Furthermore, it was noticed that the presence of both of the <em>B. thuringiensis</em><span> strains did not affect the thermal stability<span> of the particles. The microencapsulated bacterial strains<span> presented a high number of viable spores and preserved the expression of proteins with molecular masses corresponding to the insecticidal toxins Cry and Cyt, indicating that the encapsulation process was conducted satisfactorily. Finally, the encapsulated strains were tested against </span></span></span><em>Ae. aegypti</em> larvae and maintained 100% larval mortality even after 35 days. Therefore, microencapsulation of <em>B. thuringiensis</em><span> not only guarantees the bacterial activity, but also prolongs the action of the biopesticide. Collectively, such findings highlight the great potential of the new biopesticides, which may help to reduce the population indices of the mosquito vector </span><em>Ae. aegypti</em> via a sustainable and environment-friendly route.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental parasitology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014489423001959","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, we investigated the microencapsulation of two strains of the entomopathogenic bacteria Bacillus thuringiensis (B. thuringiensis) (BtMA-750 and BtMA-1114), which are biopesticides of high toxicity for the mosquito vector Aedes aegypti. The encapsulation of different concentrations of microorganisms in starch microparticles was evaluated, and the inverse suspension polymerization technique was explored. It was possible to observe that the higher amounts of the biopesticide caused a slight decrease in the diameter of the particles; however, even when encapsulated, the biopesticide still presents an average diameter that is able to be consumed by the larvae of Aedes aegypti. Furthermore, it was noticed that the presence of both of the B. thuringiensis strains did not affect the thermal stability of the particles. The microencapsulated bacterial strains presented a high number of viable spores and preserved the expression of proteins with molecular masses corresponding to the insecticidal toxins Cry and Cyt, indicating that the encapsulation process was conducted satisfactorily. Finally, the encapsulated strains were tested against Ae. aegypti larvae and maintained 100% larval mortality even after 35 days. Therefore, microencapsulation of B. thuringiensis not only guarantees the bacterial activity, but also prolongs the action of the biopesticide. Collectively, such findings highlight the great potential of the new biopesticides, which may help to reduce the population indices of the mosquito vector Ae. aegypti via a sustainable and environment-friendly route.
期刊介绍:
Experimental Parasitology emphasizes modern approaches to parasitology, including molecular biology and immunology. The journal features original research papers on the physiological, metabolic, immunologic, biochemical, nutritional, and chemotherapeutic aspects of parasites and host-parasite relationships.