Stephanie Bringer, Hans-Peter Bochem, Benno Sprey, Hermann Sahm
{"title":"Microbodies in the Brown Rot Fungus Poria contigua","authors":"Stephanie Bringer, Hans-Peter Bochem, Benno Sprey, Hermann Sahm","doi":"10.1016/S0172-5564(80)80042-X","DOIUrl":null,"url":null,"abstract":"<div><p>Using the diaminobenzidine (DAB) reaction catalase activity could be demonstrated cytochemically in cytoplasmic structures of <em>Poria contigua</em> bearing the general ultrastructural features of microbodies. These kidney-shaped compartments were associated with a densely packed coherent membrane system. When H<sub>2</sub>O<sub>2</sub> was replaced by methanol the microbodies were stained indicating that alcohol oxidase generated the H<sub>2</sub>O<sub>2</sub> for the peroxidative conversion of DAB by catalase. Based on these results we assume that catalase and alcohol oxidase are located in microbodies of this fungus as found for methanol-utilizing yeasts.</p></div>","PeriodicalId":101294,"journal":{"name":"Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","volume":"1 2","pages":"Pages 193-199"},"PeriodicalIF":0.0000,"publicationDate":"1980-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5564(80)80042-X","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S017255648080042X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Using the diaminobenzidine (DAB) reaction catalase activity could be demonstrated cytochemically in cytoplasmic structures of Poria contigua bearing the general ultrastructural features of microbodies. These kidney-shaped compartments were associated with a densely packed coherent membrane system. When H2O2 was replaced by methanol the microbodies were stained indicating that alcohol oxidase generated the H2O2 for the peroxidative conversion of DAB by catalase. Based on these results we assume that catalase and alcohol oxidase are located in microbodies of this fungus as found for methanol-utilizing yeasts.