Protein adsorption on functional hydrophilic polymer beads: role of structural properties and medium conditions

Reactive Polymers Pub Date : 1994-11-01 DOI:10.1016/0923-1137(94)90135-X

Elmas Gök , Menemşe Kiremitçi , I. Serdar Ateş

{"title":"Protein adsorption on functional hydrophilic polymer beads: role of structural properties and medium conditions","authors":"Elmas Gök , Menemşe Kiremitçi , I. Serdar Ateş","doi":"10.1016/0923-1137(94)90135-X","DOIUrl":null,"url":null,"abstract":"<div><p>Acrylic polymer beads comprising a combination of 2-hydroxyethylmethacrylate (HEMA) with acrylic acid, dimethylaminoethylmethacrylate (DMAEMA), and methylmethacrylate were prepared by suspension copolymerization technique. In this study, we have examined the effects of polymer structural properties and medium conditions on the adsorption of plasma proteins, i.e. bovine serum albumin (BSA) and fibrinogen. The studies were carried out in dilute solutions and measurements of adsorbed proteins were realized by fluorescence spectroscopy. It was shown that the extent of protein adsorption depends upon the hydrophilicity and electrical charge of both of the polymeric surfaces and proteins. In addition, environmental conditions affect the adsorption behaviour by changing the structure of protein molecules. The maximum adsorption was observed on poly(HEMA-DMAE) beads for both of BSA and fibrinogen. For this polymeric structure multilayer BSA adsorption was observed in the range of 0.1–0.5 mg/ml solution concentration. For all surfaces, the adsorbed quantity of fibrinogen was greater than that of BSA.</p></div>","PeriodicalId":20864,"journal":{"name":"Reactive Polymers","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1994-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0923-1137(94)90135-X","citationCount":"16","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reactive Polymers","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/092311379490135X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 16

Abstract

Acrylic polymer beads comprising a combination of 2-hydroxyethylmethacrylate (HEMA) with acrylic acid, dimethylaminoethylmethacrylate (DMAEMA), and methylmethacrylate were prepared by suspension copolymerization technique. In this study, we have examined the effects of polymer structural properties and medium conditions on the adsorption of plasma proteins, i.e. bovine serum albumin (BSA) and fibrinogen. The studies were carried out in dilute solutions and measurements of adsorbed proteins were realized by fluorescence spectroscopy. It was shown that the extent of protein adsorption depends upon the hydrophilicity and electrical charge of both of the polymeric surfaces and proteins. In addition, environmental conditions affect the adsorption behaviour by changing the structure of protein molecules. The maximum adsorption was observed on poly(HEMA-DMAE) beads for both of BSA and fibrinogen. For this polymeric structure multilayer BSA adsorption was observed in the range of 0.1–0.5 mg/ml solution concentration. For all surfaces, the adsorbed quantity of fibrinogen was greater than that of BSA.

查看原文本刊更多论文

蛋白质在功能性亲水性聚合物珠上的吸附:结构性质和介质条件的作用

采用悬浮共聚技术制备了由2-羟乙基甲基丙烯酸酯(HEMA)与丙烯酸、二甲氨基乙基甲基丙烯酸酯(DMAEMA)和甲基丙烯酸甲酯组成的丙烯酸聚合物珠。在这项研究中，我们研究了聚合物结构性质和介质条件对血浆蛋白(即牛血清白蛋白(BSA)和纤维蛋白原)吸附的影响。研究是在稀释溶液中进行的，吸附蛋白的测量是通过荧光光谱实现的。结果表明，蛋白质的吸附程度取决于聚合物表面和蛋白质的亲水性和电荷。此外，环境条件通过改变蛋白质分子的结构来影响吸附行为。聚HEMA-DMAE对BSA和纤维蛋白原的吸附均达到最大。该聚合物结构在0.1 ~ 0.5 mg/ml溶液浓度范围内对BSA有多层吸附。纤维蛋白原在各表面的吸附量均大于BSA。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Reactive Polymers

自引率

0.00%

发文量