Recomendaciones preanalíticas para la obtención y análisis de ADN circulante a partir de sangre periférica. Recomendación (2018)

Revista del Laboratorio Clínico Pub Date : 2019-07-01 DOI:10.1016/j.labcli.2018.11.002

Álvaro González , Clara Pérez Barrios , Hada Macher , Marta Sánchez-Carbayo , Antonio Barco Sánchez , Antonio Fernández Suárez , Antonio Martínez Peinado , Blanca Ortiz Muñoz , Jaume Trapé Pujol , José Diego Santotoribio Camacho , Josep María Augé Fradera , María Jesús Gaspar Blázquez , Rosa Carbonell Muñoz , Xavier Filella Pla , Comité Científico de la Sociedad Española de Medicina de Laboratorio (SEQCML)

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Abstract

Cell-free DNA analysis in peripheral blood has been shown to be useful in oncology, organ transplantation, and prenatal screening. For its introduction into the clinical laboratory, it is necessary to ensure appropriate, reproducible and standardised pre-analytical protocols are in place. The aim of this document is to provide pre-analytical recommendations for obtaining of cell free DNA from peripheral blood. These recommendations include the type of sample and extraction tube, the method of centrifugation, the method for cell free DNA extraction, and measurement and storage conditions.

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从外周血中获取和分析循环dna的分析前建议。建议(2018年)

游离DNA分析已被证明在肿瘤学、器官移植和产前筛查中是有用的。为了将其引入临床实验室，有必要确保有适当的、可重复的和标准化的分析前方案。本文的目的是为从外周血中获得游离细胞DNA提供分析前建议。这些建议包括样品类型和提取管，离心方法，细胞游离DNA提取方法，以及测量和储存条件。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Revista del Laboratorio Clínico

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