Phloretin-sensitive lithium transport in erythrocytes of affectively ill patients: Intra-individual reproducibility

Eva S. Werstiuk, Michel P. Rathbone, Paul Grof
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引用次数: 10

Abstract

  • 1.

    1. The phloretin-sensitive component of lithium (Li+) efflux from erythrocytes has been reported to be significantly reduced in patients with bipolar affective disorders. The value of this finding depends on how reproducible the efflux measurements are. We have therefore investigated the intra-individual day-to-day reproducibility of this parameter, and total erythrocyte Li+ efflux in patients with bipolar affective disorders and in age and sex matched healthy controls.

  • 2.

    2. Six carefully selected, typical bipolar patients and age and sex matched healthy volunteers were studied. These experiments were repeated under identical conditions on 3 occasions within a brief time period (21 days).

  • 3.

    3. We have found that both the total erythrocyte Li+ efflux and the phloretin-sensitive Li+ efflux exhibited considerable day-to-day intra-individual variability in both patients and healthy controls. The standard deviation of total efflux values was as large as ± 31.1% of the mean in some subjects. Mean phloretin-sensitive Li+ efflux for patients was found to be 0.066 ± 0.02 mEq/1 RBC, and for controls 0.048 ± 0.01 mEq/1 RBC, in agreement with values reported in the literature. However, in some individuals standard deviation of this parameter, was as high as ± 50% of the mean. This variation was not the result of the biochemical technique, since all measurements were carried out in duplicate, and these differed less than 5%.

  • 4.

    4. These findings suggest, that in view of the small absolute values of the phloretin-sensitive Li+ efflux, and its considerable day-to-day variation, the relevance of this biochemical parameter to the postulated cell membrane defect in affective disorders should be interpreted with some caution and needs further evaluation.

患病患者红细胞中酞菁醛敏感锂转运:个体内可重复性
1.1. 据报道,在双相情感障碍患者中,红细胞外排锂(Li+)的内皮素敏感成分显着减少。这一发现的价值取决于外排测量的可重复性。因此,我们研究了该参数的个体内部日常再现性,以及双相情感障碍患者和年龄和性别匹配的健康对照组的红细胞总Li+外排。2.2。六名精心挑选的典型双相情感障碍患者以及年龄和性别匹配的健康志愿者进行了研究。在短时间内(21 d),在相同条件下重复3次实验。我们发现,在患者和健康对照中,红细胞总Li+外排和对韧皮素敏感的Li+外排都表现出相当大的日常个体内变异性。部分受试者总流出值的标准差高达平均值的±31.1%。患者皮绿素敏感Li+外排平均值为0.066±0.02 mEq/1个RBC,对照组为0.048±0.01 mEq/1个RBC,与文献报道值一致。然而,在某些个体中,该参数的标准差高达平均值的±50%。这种差异不是生化技术的结果,因为所有的测量都是重复进行的,这些差异小于5%。这些发现表明,鉴于皮绿素敏感的Li+外排的绝对值很小,而且它的日常变化很大,这一生化参数与情感性障碍中假定的细胞膜缺陷的相关性应该谨慎解释,需要进一步评估。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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