Neuronal and astrocytic protein degradation are critical for fear memory formation.

IF 1.8 4区医学 Q4 NEUROSCIENCES

Learning & memory Pub Date : 2023-03-15 Print Date: 2023-03-01 DOI:10.1101/lm.053716.122

Kayla Farrell, Taylor McFadden, Timothy J Jarome

引用次数: 0

Abstract

Strong evidence has implicated proteasome-mediated protein degradation in the memory consolidation process. However, due to the use of pharmacological approaches, the cell type specificity of this remains unknown. Here, we used neuron-specific and novel astrocyte-specific CRISPR-dCas9-KRAB-MECP2 plasmids to inhibit protein degradation in a cell type-specific manner in the amygdala of male rats. We found that while inhibition of neuronal, but not astrocytic, protein degradation impaired performance during the training session, both resulted in impaired contextual fear memory retention. Together, these data provide the first evidence of a cell type-specific role for protein degradation in the memory consolidation process.

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神经元和星形胶质细胞的蛋白质降解对恐惧记忆的形成至关重要。

大量证据表明，蛋白酶体介导的蛋白质降解与记忆巩固过程有关。然而，由于药理学方法的使用，其细胞类型特异性仍然未知。在这里，我们使用神经元特异性和新型星形胶质细胞特异性 CRISPR-dCas9-KRAB-MECP2 质粒，以细胞类型特异性的方式抑制雄性大鼠杏仁核中的蛋白质降解。我们发现，抑制神经元而非星形胶质细胞的蛋白降解会损害训练过程中的表现，但两者都会导致情境恐惧记忆的保留受损。这些数据首次证明了蛋白质降解在记忆巩固过程中具有细胞类型特异性的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Learning & memory 医学-神经科学

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The neurobiology of learning and memory is entering a new interdisciplinary era. Advances in neuropsychology have identified regions of brain tissue that are critical for certain types of function. Electrophysiological techniques have revealed behavioral correlates of neuronal activity. Studies of synaptic plasticity suggest that some mechanisms of memory formation may resemble those of neural development. And molecular approaches have identified genes with patterns of expression that influence behavior. It is clear that future progress depends on interdisciplinary investigations. The current literature of learning and memory is large but fragmented. Until now, there has been no single journal devoted to this area of study and no dominant journal that demands attention by serious workers in the area, regardless of specialty. Learning & Memory provides a forum for these investigations in the form of research papers and review articles.