МОДЕЛИРОВАНИЕ ВИРУСНОГО ГЕПАТИТА Е НА КАРЛИКОВЫХ ДОМАШНИХ СВИНЬЯХ

Q3 Medicine

Zhurnal mikrobiologii, epidemiologii, i immunobiologii Pub Date : 2017-08-28 DOI:10.36233/0372-9311-2017-4-48-54

S. A. Gulyaev, I. A. Potemkin, V. S. Kichatova, A. A. Karlsen, O. V. Isaeva, T. V. Guiyaeva, M. A. Vannus, I. V. Gordeichuk, K. K. Kyuregyan, M. I. Mikhailov

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引用次数: 2

Abstract

Aim. Experimental reproduction of HEV-infection in mini-pigs and comparative analysis of virological and immunological characteristics of experimental infection. Materials and methods. Wiesenauer minipigs (2 females and 4 males, age 50 - 60 days, weight 5-10 kg) were infected by HEV genotype 3 strain isolated from swine feces in Belgorod region in 2013. The virus was administered as a 10% clarified feces extract (800 pi). Blood (weekly) and feces (daily) were sampled from the animals for 49 days. Anti-HEV IgG were determined in sera samples using DS- ElA-ANTI-HEV-G (Diagnostic Systems) system, HEV RNA in samples of feces extracts and blood sera - RT-PCR. Results. Wiesenauer minipigs were sensitive to HEV genotype 3 infection, infection developed in all the animals after administration of the virus, that was accompanied by the presence of HEV RNA in feces for 2 weeks and seroconversion by anti-HEV. Comparative analysis of virological characteristics of experimental H EV-infection in mini- and standard pigs has shown, that the duration of the infection in mini-pigs is shorter, and seroconversion by anti-HEV occurs earlier. Conclusion. Sensitivity to HEV genotype 3 and lower weight make mini-pigs a comfortable alternative to standard swine and primates for modelling HEV infection in vivo.

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侏儒家畜病毒型肝炎模拟

的目标。小型猪hev感染的实验繁殖及实验感染的病毒学和免疫学特征的比较分析。材料和方法。2013年，从别尔哥罗德地区猪粪中分离出HEV基因3型毒株，感染了Wiesenauer小型猪(2母4公，50 ~ 60日龄，体重5 ~ 10 kg)。该病毒以10%澄清粪便提取物(800 pi)的形式施用。采集动物血液(每周一次)和粪便(每天一次)，为期49天。采用DS- ElA-ANTI-HEV-G (Diagnostic Systems)系统检测血清中抗HEV IgG、粪便提取物和血清中HEV RNA。结果。Wiesenauer小型猪对HEV基因3型感染敏感，所有动物在给药后均出现感染，并伴有粪便中存在HEV RNA 2周，并通过抗HEV血清转化。对小型猪和标准猪实验型hev感染的病毒学特征进行对比分析，发现小型猪感染持续时间较短，抗hev血清转化发生时间较早。结论。对HEV基因型3的敏感性和较低的体重使迷你猪成为标准猪和灵长类动物在体内模拟HEV感染的舒适选择。

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