Immune Responses Are Differentially Regulated by Root, Stem, Leaf, and Flower Extracts of Female and Male CBD Hemp (Cannabis sativa L.) Plants

Immuno-Analyse & Biologie Specialisee Pub Date : 2021-10-22 DOI:10.3390/immuno1040025

Laura G. A. Esposito, E. Overbaugh, J. Xiong, Thirumurugan Rathinasabapathy, S. Komarnytsky, D. D. da Silva, D. Esposito

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引用次数: 3

Abstract

Industrial hemp (Cannabis sativa L.) has many applications, including the production of textiles, agricultural extracts, nutritional products, and botanicals enriched with cannabinoids and full-spectrum terpenes naturally present in the plant. In this study, the dynamics of distribution and accumulation of 10 main cannabinoids in hemp were quantified. Hemp bioactive compounds were evaluated for anti-inflammatory activity in lipopolysaccharide-induced RAW 264.7 macrophage cells. While all tissues of hemp showed moderate anti-inflammatory properties, female flowers demonstrated the highest activity. CBD showed the strongest anti-inflammatory activity with suppression of nitric oxide production at 2 μg/mL and the reduced expressions of the pro-inflammatory genes COX-2, IL-6, and TNF-α at as low as 2 ng/mL. The topical hemp inflorescences (1–50 μg/mL) and CBD alone (20–200 ng/mL) also improved mitochondrial respiration. These data contribute to the future development of agricultural and plant management techniques to produce hemp with specific metabolite profiles to selectively support immune health.

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雌雄CBD大麻(Cannabis sativa L.)根、茎、叶和花提取物对免疫反应的差异调节植物

工业大麻(Cannabis sativa L.)有许多用途，包括生产纺织品、农业提取物、营养品和富含大麻素和天然存在于植物中的全谱萜烯的植物药。本研究对大麻中10种主要大麻素的分布和积累动态进行了定量研究。研究了大麻生物活性化合物对脂多糖诱导的RAW 264.7巨噬细胞的抗炎活性。大麻各组织均表现出中等抗炎活性，以雌花活性最高。CBD表现出最强的抗炎活性，在2 μg/mL时抑制一氧化氮的产生，低至2 ng/mL时降低促炎基因COX-2、IL-6和TNF-α的表达。外用大麻花序(1 ~ 50 μg/mL)和单独使用CBD (20 ~ 200 ng/mL)也能改善线粒体呼吸。这些数据有助于未来农业和植物管理技术的发展，以生产具有特定代谢物谱的大麻，以选择性地支持免疫健康。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immuno-Analyse & Biologie Specialisee 医学-医学实验技术

自引率

0.00%

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审稿时长

6-12 weeks