Endogenous Levels of Abscisic Acid in Embryogenic Cells, Nonembryogenic Cells and Somatic Embryos of Carrot (Daucus carota L.)

Tomohiro Kiyosue , Masatoshi Nakajima , Isomaro Yamaguchi , Shinobu Satoh , Hiroshi Kamada , Hiroshi Harada
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引用次数: 32

Abstract

The levels of endogenous ABA were measured by ELISA in embryogenic cells, nonembryogenic cells and somatic embryos of carrot. Embryogenic cells were estimated to contain a 67.2 times higher level of ABA than non-embryogenic cells, and a 2.5 times higher level than somatic embryos. The correlation between the endogenous level of ABA and embryogenic competence of carrot cultured cells is discussed.

胡萝卜胚性细胞、非胚性细胞和体胚中脱落酸内源水平的研究
采用ELISA法测定了胡萝卜胚性细胞、非胚性细胞和体胚中内源ABA的含量。据估计,胚性细胞的ABA含量比非胚性细胞高67.2倍,比体细胞胚胎高2.5倍。探讨了内源ABA水平与胡萝卜培养细胞胚发生能力的关系。
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