Mouse vaginal development with lateral enlargement at late embryonic stages and caudal elongation after birth

IF 1.3 4区 医学 Q3 PEDIATRICS
Masayo Harada, Keiichi Akita
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Abstract

Müllerian ducts give rise to the oviducts, uterus, cervix, and vagina. During female reproductive tract development in mice, the bilateral Müllerian duct epithelium grows caudally until reaching the urogenital sinus epithelium. This is followed by further caudal growth with the reduction of the urogenital sinus epithelium. Finally, the vaginal epithelium of adult mice is entirely derived from the Müllerian duct epithelium. Here, we explored the mechanisms underlying mouse vaginal development via cell proliferation, apoptosis, and lineage analyses. We found that at the late embryonic stages, apoptosis occurred at the attachment site of bilateral Müllerian duct epithelia below the cervix, resulting in bilateral lumen traffic. The Müllerian duct epithelium was enclosed by the urogenital sinus epithelium at their boundary region on embryonic day (E) 16.5, whereas the Müllerian duct epithelium encased the urogenital sinus epithelium at postnatal day (P) 0 through lateral enlargement. Lateral Müllerian duct enlargement was accompanied by focal ERK activation within the curved epithelial tips and the specific localization of mitotic nuclei on the luminal side of the Müllerian duct epithelial layer at E17.5. Descent of the Müllerian duct epithelium and shortening of the urogenital sinus epithelium occurred rapidly after birth, accompanied by cell proliferation in the Müllerian duct epithelium and its peripheral mesenchymal tissues as well as intense apoptosis in the urogenital sinus epithelium around their boundary region. Urogenital sinus epithelium was localized at the base of the vagina at P7. In conclusion, the mouse vagina develops laterally at the late embryonic stages and caudally after birth.

Abstract Image

小鼠阴道发育,胚胎后期侧边增大,出生后尾端伸长
输卵管由输卵管、子宫、子宫颈和阴道组成。在小鼠雌性生殖道发育过程中,双侧肋管上皮向尾侧生长,直至到达泌尿生殖窦上皮。随后是进一步的尾端生长,伴有泌尿生殖窦上皮的减少。最后,成年小鼠阴道上皮完全来源于勒管上皮。在这里,我们通过细胞增殖、细胞凋亡和谱系分析探讨了小鼠阴道发育的机制。我们发现,在胚胎晚期,子宫颈下双侧勒管上皮附着部位发生细胞凋亡,导致双侧管腔交通。胚胎日(E) 16.5时,腰勒氏管上皮在其边界区域被泌尿生殖窦上皮包围,而出生后(P) 0日,腰勒氏管上皮通过外侧增大包裹了泌尿生殖窦上皮。在E17.5时,腰勒管增大伴随着弯曲上皮尖端内的局灶性ERK激活,有丝分裂核特异性定位于腰勒管上皮层管腔侧。出生后,臀勒氏管上皮迅速下降,泌尿生殖窦上皮缩短,伴随着臀勒氏管上皮及其周围间质组织的细胞增殖,以及其交界区周围的泌尿生殖窦上皮细胞强烈凋亡。尿生殖窦上皮位于阴道底部P7。总之,小鼠阴道在胚胎后期向外侧发育,出生后向尾侧发育。
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来源期刊
Congenital Anomalies
Congenital Anomalies PEDIATRICS-
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Congenital Anomalies is the official English language journal of the Japanese Teratology Society, and publishes original articles in laboratory as well as clinical research in all areas of abnormal development and related fields, from all over the world. Although contributions by members of the teratology societies affiliated with The International Federation of Teratology Societies are given priority, contributions from non-members are welcomed.
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