Isolation and Characterization of Two Genes Encoding Ubiquitin Fused to a Ribosomal Protein of 53 Amino Acids in Rice

A. Kato, Ritsuko Nishi And, Masahiko Ozak1
{"title":"Isolation and Characterization of Two Genes Encoding Ubiquitin Fused to a Ribosomal Protein of 53 Amino Acids in Rice","authors":"A. Kato, Ritsuko Nishi And, Masahiko Ozak1","doi":"10.3109/10425170109042050","DOIUrl":null,"url":null,"abstract":"We isolated and determined the nucleotide sequences of two genes encoding ubiquitin fused to a ribosomal protein, Ub-CEP52, from rice (Oryza saliva L.). The deduced amino-acid sequences of the two genes were found to be completely identical. The N-terminal region of 76 residues corresponds to ubiquitin, and the C-terminal region of 53 residues corresponds to ribosomal protein L40. A putative TATA-like sequence, a polypyrimidine sequence, and a similar sequence to telo-box were found in the promoter regions of the two genes. Furthermore, the putative tRNA ro gene was found in the 5′-upstream region of one of them.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"1 1","pages":"53 - 58"},"PeriodicalIF":0.0000,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"DNA Sequence","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10425170109042050","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3

Abstract

We isolated and determined the nucleotide sequences of two genes encoding ubiquitin fused to a ribosomal protein, Ub-CEP52, from rice (Oryza saliva L.). The deduced amino-acid sequences of the two genes were found to be completely identical. The N-terminal region of 76 residues corresponds to ubiquitin, and the C-terminal region of 53 residues corresponds to ribosomal protein L40. A putative TATA-like sequence, a polypyrimidine sequence, and a similar sequence to telo-box were found in the promoter regions of the two genes. Furthermore, the putative tRNA ro gene was found in the 5′-upstream region of one of them.
水稻53个氨基酸核糖体蛋白泛素编码基因的分离与鉴定
我们从水稻(Oryza唾液L.)中分离并测定了编码泛素融合到核糖体蛋白Ub-CEP52的两个基因的核苷酸序列。推断出的两个基因的氨基酸序列完全相同。76个残基的n端区域对应泛素,53个残基的c端区域对应核糖体蛋白L40。在两个基因的启动子区域发现了一个推测的TATA-like序列、一个聚嘧啶序列和一个类似于telo-box的序列。此外,在其中一个的5 '上游区域发现了假定的tRNA ro基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信