Comparative reproductive biology of two sympatric Hypostomus in a Neotropical river

Abstract

Congeneric species often coexist in sympatry using behavioral and morphological adaptations to reduce competition and interspecific interference, but reproductive patterns behind coexistence remain unknown. We analyzed the gonadal morphology and development, reproductive cycle, and population structure of two sympatric congeneric fishes to evaluate the degree of overlap and differentiation of the reproductive biology between species in a Neotropical river. Development of testes and ovaries were similar between species, both showing asynchronous gonadal development, large diameter of gametes and synthesis of mucosubstances by follicle cells to form adhesive eggs. Although the morphometry of germ cells did not present differences, the zona radiata of mature eggs in Hypostomus garmani was markedly thicker than H. francisci, which suggests different spawning habitats. Both species have greater reproductive activity in the rainy season, concomitant with increase in water temperature, however H. garmani initiates and ends its reproduction earlier than H. francisci, indicating a differentiation of reproductive periods. Sexually mature males and females of H. francisci reproduced at a larger mean size then H. garmani. The two congeneric species had a similar abundance and sex ratios in the study area. Results show that although the species exhibited broad overlap of reproductive traits, a spatial and temporal differentiation of the reproductive biology was present. This study contributes to understanding reproductive mechanisms that may facilitate coexistence between congeneric sympatric species.