T. Mahmood, H. Arshad, W. Akhtar, M. Ilyas, Tariq Habib, Z. Shinwari
{"title":"Tissue culture optimization for Lallementia royleana L. an important medicinal plant","authors":"T. Mahmood, H. Arshad, W. Akhtar, M. Ilyas, Tariq Habib, Z. Shinwari","doi":"10.21826/2446-82312021V76E2021009","DOIUrl":null,"url":null,"abstract":"This study was designed to develop an efficient protocol for Lallementia royleana in vitro culturing and to analyze the effect of various hormones on its culturing ability. The indirect organogenesis and its callogenetic ability were evaluated; with the best (100%) results with 1 mg L-1 of benzyl aminopurine (BAP) and 2 mg L-1 of indole acetic acid (IAA) combination. In direct regeneration, leave petioles, nodes and internodes explants were cultured for shooting on various concentrations of BAP and kinetin (KIN). Maximum shooting (80%) was obtained from nodal parts with 1.0 mg L-1 of BAP and 0.5 mg L-1 of KIN. Similarly, the best in vitro rooting (24 roots) was achieved with 1 mg L-1 naphthalene acetic acid (NAA). Lallementia royleana showed higher regeneration through direct regeneration than callogenesis. Significant variability (p = 0.002625) among callus induction, plant parts and hormonal combinations were observed. The node and internode explants showed observable callus responses in combinations of 2, 4-Dichlorophenoxyacetic acid (2,4-D) with NAA, and BAP with IAA.","PeriodicalId":54998,"journal":{"name":"Iheringia Serie Botanica","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iheringia Serie Botanica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.21826/2446-82312021V76E2021009","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
This study was designed to develop an efficient protocol for Lallementia royleana in vitro culturing and to analyze the effect of various hormones on its culturing ability. The indirect organogenesis and its callogenetic ability were evaluated; with the best (100%) results with 1 mg L-1 of benzyl aminopurine (BAP) and 2 mg L-1 of indole acetic acid (IAA) combination. In direct regeneration, leave petioles, nodes and internodes explants were cultured for shooting on various concentrations of BAP and kinetin (KIN). Maximum shooting (80%) was obtained from nodal parts with 1.0 mg L-1 of BAP and 0.5 mg L-1 of KIN. Similarly, the best in vitro rooting (24 roots) was achieved with 1 mg L-1 naphthalene acetic acid (NAA). Lallementia royleana showed higher regeneration through direct regeneration than callogenesis. Significant variability (p = 0.002625) among callus induction, plant parts and hormonal combinations were observed. The node and internode explants showed observable callus responses in combinations of 2, 4-Dichlorophenoxyacetic acid (2,4-D) with NAA, and BAP with IAA.
期刊介绍:
The Iheringia, Série Botânica is a publication edited by the Museu de Ciências Naturais, Fundação Zoobotânica do Rio Grande do Sul, since 1958. The name Iheringia is actually a tribute to Hermann von Ihering (a German researcher who became a Brazilian citizen) and his important studies in the Natural Sciences.The journal has the mission of publishing articles, reviews and original scientific notes that cover diverse areas in Botany, and may be written in Portuguese, Spanish and English.
The journal is widely available in both international and national scientific community. The issues are published and distributed twice a year to 118 national institutions and 152 international institutions. The distribution is either free of charge or by exchange.
Impac Factor JCR 2014/2015 = 0.263
Qualis CAPES 2015: B 4 (Biodiversity) B1 (Agricultural Science I) B1 (Environment)