Construction of Plant Regeneration System of Glycyrrhiza mongolicum and Glycyrrhiza Xinjiang

Gao Xinxin, Tian Yu, Guoliang Chen, Wu Jiawen, Bai Zhenqing
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Abstract

In order to establish the plant regeneration system of  Glycyrrhiza mongolicum  and  Glycyrrhiza  Xinjiang, this study used hypocotyls, leaves, cotyledons and stems to inducing callus and regenerated plant, and it screen out the most suitable explants and the best medium for plant regeneration. The experimental results showed that MS+2.0 mg/L NAA+2.0 mg/L 6-BA was the best medium for callus induction and plant regeneration. Among these, hypocotyls and cotyledons are the best explants for callus induction. The tissue induction rate is higher than 90%. The rapid propagation system established in this study can provide the theoretical reference and practical guidance for the breeding and production of fine  Glycyrrhiza uralensis  Fisch. varieties.
蒙古甘草与新疆甘草植株再生体系的构建
为了建立蒙古甘草和新疆甘草的植株再生体系,本研究利用下胚轴、叶片、子叶和茎诱导愈伤组织和再生植株,筛选出最适合植株再生的外植体和最佳培养基。结果表明,MS+2.0 mg/L NAA+2.0 mg/L 6-BA是愈伤组织诱导和植株再生的最佳培养基。其中,下胚轴和子叶是诱导愈伤组织的最佳外植体。组织诱导率大于90%。本研究建立的快速繁殖体系可为优质乌拉尔甘草的选育和生产提供理论参考和实践指导。品种。
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