Chemical composition and antioxidant activity of immortelle ('Helichrysum italicum') (Roth) G. Don) and yarrow ('Achillea millefolium' L.) essential oils

J. Stanojević, L. Stanojević, V. Bulatović, J. Zvezdanović, A. Milenković, Nataša Simonović, D. Cvetković
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引用次数: 2

Abstract

The present study aimed to determine the chemical composition and antioxidant activity of immortelle (Helichrysum italicum (Roth) G. Don) and yarrow (Achillea millefolium L.) essential oils isolated from the aerial plant parts on a semi-industrial scale by steam and water-steam distillation, respectively. The qualitative composition of the essential oils obtained was determined by GC/MS and their quantitative composition by GC/FID method. Their antioxidant activity was estimated by using DPPH, ABTS and FRAP assays. The most abundant components in the immortelle essential oil were a-pinene (22.4%), g-curcumene (13.2%), neryl acetate (9.8%) and b-selinene (8.7%), while yarrow essential oil contained 1,8-cin-eole (25.5%), CIS-thujone (10.8%), camphor (7.3%) and artemisia ketone (6.9%) in the highest percentage. According to the EC 50 values yarrow essential oil showed better radical (DPPH and ABTS) scavenging activity than immortelle essential oil (7.71 mg/cm 3 vs. 8.94 mg/cm 3 after 20 minutes of incubation with DPPH radical ; and 26.03 mg/cm 3 vs. 88.52 mg/cm 3 after 24 minutes incubation with ABTS radical, respectively). On the other hand, immortelle essential oil showed better ferric ion reducing power (7.16 mgEFe2+/g vs. 5.72 mgEFe2+/g essential oil) in comparison to yarrow essential oil. However, the results obtained indicated a low antioxidant activity of both essential oils studied.
不朽草('Helichrysum italicum') (Roth) G. Don)和蓍草('Achillea millefolium' L.)精油的化学成分和抗氧化活性
采用蒸汽蒸馏法和水-蒸汽蒸馏法对半工业条件下从空中植物部位分离得到的长青草(Helichrysum italicum (Roth) G. Don)和蓍草(Achillea millefolium L.)精油的化学成分和抗氧化活性进行了研究。采用气相色谱/质谱法对所得精油进行定性分析,采用气相色谱/FID法对所得精油进行定量分析。采用DPPH、ABTS和FRAP测定其抗氧化活性。长青挥发油中含量最高的成分为a-蒎烯(22.4%)、g-姜黄烯(13.2%)、neryl acetate(9.8%)和b-selinene(8.7%),而蓍草挥发油中含量最高的成分为1,8- cine -eole(25.5%)、顺子图琼(10.8%)、樟脑(7.3%)和蒿酮(6.9%)。根据EC 50值,蓍草精油对DPPH和ABTS自由基的清除能力优于长青精油(DPPH自由基作用20 min后,分别为7.71 mg/cm 3和8.94 mg/cm 3);与ABTS自由基孵育24分钟后分别为26.03 mg/ cm3和88.52 mg/ cm3)。另一方面,与蓍草精油相比,不朽精油具有更好的铁离子还原能力(7.16 mgEFe2+/g比5.72 mgEFe2+/g)。然而,得到的结果表明,这两种精油的抗氧化活性都很低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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