Nitric Oxide Inhibitory Activity of Crude Extracts and Extraction Time and Temperature Optimization of Pectin from Cissampelos pareira Leaves

IF 0.6 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

Chiang Mai Journal of Science Pub Date : 2023-07-31 DOI:10.12982/cmjs.2023.043

La-or Somsakeesit, Watcharin Chumjan, S. Suwannapan, Acharaporn Ratanamanee

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引用次数: 0

Abstract

This research studied crude extracts of Cissampelos pareira leaves using fi ve solvents as hexane, dichloromethane, ethyl acetate, methanol, and ethanol for nitric oxide (NO) inhibitory activity and maximize pectin yields from facile green extraction. Five crude hexane, dichloromethane, ethyl acetate, methanol and ethanol extracts were obtained from the leaves of C. pareira at 0.4%, 0.5%, 0.3%, 3%, and 1%, respectively. The hexane, dichloromethane, and ethyl acetate extracts showed the most potent decrease in NO production with half-maximal inhibitory concentration (IC50) values of 49.85±5.07, 56.26±1.49, and 64.55±2.37 (μg/mL), respectively. Concentrations of all crude extracts of C. pareira indicated no cellular toxicity. Pectin was aqueously extracted at 40 to 90 °C for 15 to 120 min and yields and quality were examined. Highest pectin yield (36.12%) was obtained after 60 min at 60 °C, with quality meeting the criteria established by the International Pectin Producers Association (IPPA) and categorized as high methoxyl pectin.

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山梨叶果胶粗提物抑制一氧化氮活性及提取时间和温度优化

采用正己烷、二氯甲烷、乙酸乙酯、甲醇和乙醇5种溶剂，研究了西桑叶粗提物对一氧化氮(NO)的抑制活性，并最大限度地提高了绿色提取中果胶的产量。分别以0.4%、0.5%、0.3%、3%和1%的浓度从白鹤叶中提取粗己烷、二氯甲烷、乙酸乙酯、甲醇和乙醇5种提取物。正己烷、二氯甲烷和乙酸乙酯提取物对NO的抑制作用最强，半抑制浓度(IC50)分别为49.85±5.07、56.26±1.49和64.55±2.37 (μg/mL)。所有粗提物的浓度均显示无细胞毒性。在40 ~ 90℃水浸15 ~ 120 min，考察果胶的收率和质量。在60℃下发酵60 min，果胶收率最高(36.12%)，质量符合国际果胶生产者协会(IPPA)制定的标准，属于高甲氧基果胶。

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来源期刊

Chiang Mai Journal of Science MULTIDISCIPLINARY SCIENCES-

CiteScore

1.00

自引率

25.00%

发文量

103

审稿时长

3 months

期刊介绍： The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.