Phytochemical screening and high-performance thin-layer chromatography profile of Sargassum wightii and its antioxidant activity

Abstract

Objective: The present study was aimed to screening the phytochemical constituents present in various solvent extracts such as hexane, chloroform, acetone, ethyl acetate, and methanol of Sargassum wightii. Selected extract was subjected to high-performance thin-layer chromatography (HPTLC) profile and its antioxidant activity. Materials and Methods: All extracts were screening of phytochemicals using standard procedures as described by Harborne. The maximum constituents contained in extract were subjected to identify the functional groups using FT-IR and also subjected for HPTLC screening with suitable mobile phase as hexane:toluene:chloroform:ethyl acetate. The HPTLC fingerprint exhibited the bands of phytochemical constituents and was visualized under UV 254 nm and 366 nm. TLC spots were scanned by CAMAG TLC scanner. Anti-oxidant activity of ethyl acetate extract was determined by DPPH free-radical scavenging activity. Results: Ethyl acetate extract was contained maximum number of components such as alkaloids, phenols, steroids, saponins, flavonoids, and tannins. HPTLC chromatogram of Ethyl acetate extract recorded seven bands at 254 nm with different Rf values whereas at 366 nm, 12 bands were recorded with maximum Rf values. The DPPH assay indicates S. wightii as a potential antioxidant. IC50 value of S. wightii is 320.05 µg/ml. Conclusion: The results suggest that S. wightii has an edible source for many nutrients show potential antioxidant activity