Determination of Copper Tripeptide-1 in Cosmetics by High Performance Liquid Chromatography

Abstract

A method for the determination of copper tripeptide-1 in cosmetics by HPLC was established. The pretreatment of samples and chromatographic conditions were optimized respectively. It was found that demulsifiers were not necessary for aqueous cosmetics, and demulsifiers were needed for emulsified cosmetics to extract the target substances completely. The results of methodology investigation showed that the copper content of tripeptide-1 was linear within the range of 5-500 mg/L, the correlation coefficient was 0.9999, the recovery was 92.5%~99.6%, the relative standard deviation (RSD) was 2.27%~3.25%, and the detection limit was 10 mg/kg. The experimental