Selectin P (PADGEM GMP-140)—Mediated Adhesion of Human Platelets to Neutrophils in Vitro and the Immune Complex-induced Peritoneitis in Rats is Influenced by Interleukin-8

Endothelium-journal of Endothelial Cell Research Pub Date : 1995-01-01 DOI:10.3109/10623329509024669

A. Dembińska-kieć, O. Wenhrynowicz, M. Telesz, A. Zmuda, J. Stachura, M. Burchert, B. Peskar, R. Gryglewski

{"title":"Selectin P (PADGEM GMP-140)—Mediated Adhesion of Human Platelets to Neutrophils in Vitro and the Immune Complex-induced Peritoneitis in Rats is Influenced by Interleukin-8","authors":"A. Dembińska-kieć, O. Wenhrynowicz, M. Telesz, A. Zmuda, J. Stachura, M. Burchert, B. Peskar, R. Gryglewski","doi":"10.3109/10623329509024669","DOIUrl":null,"url":null,"abstract":"The expression of Selectin-P was measured in terms of formation of “rosettes” by human gell-filtrated, thrombin (30-50 mU)—stimulated platelets on the surface of isolated homologous neutrophilic leukocytes (PMNs) according to Jungi (1986). The monoclonal anti-Selectin P-antibody completely prevented formation of “rosettes” proving the specificity of Selectin-P mediation of adhesion. IL-8 (50–200 ng/ml) concentration-dependently inhibited the adhesion of platelets to PMNs. Neither Aspirin nor L-NO2Arg, modified the inhibitory activity of IL-8. The Ova-antyOva-complex-induced peritoneitis in rats was associated with the accumulation of PMNs and protein in abdominal cavity as soon as after 2 hours after i.p. injection of complexes. The pretreatment of rats with IL-8 (1 μg/300g) decreased the number of PMNs migrating to abdominal cavity, and tended to decrease their ability to synthetise NO. The suggested by Gimbrone et al. (1989) anti-inflammatory properties of a circulating form of IL-8 seems to be related ...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"31 2 1","pages":"235-241"},"PeriodicalIF":0.0000,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Endothelium-journal of Endothelial Cell Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10623329509024669","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

The expression of Selectin-P was measured in terms of formation of “rosettes” by human gell-filtrated, thrombin (30-50 mU)—stimulated platelets on the surface of isolated homologous neutrophilic leukocytes (PMNs) according to Jungi (1986). The monoclonal anti-Selectin P-antibody completely prevented formation of “rosettes” proving the specificity of Selectin-P mediation of adhesion. IL-8 (50–200 ng/ml) concentration-dependently inhibited the adhesion of platelets to PMNs. Neither Aspirin nor L-NO2Arg, modified the inhibitory activity of IL-8. The Ova-antyOva-complex-induced peritoneitis in rats was associated with the accumulation of PMNs and protein in abdominal cavity as soon as after 2 hours after i.p. injection of complexes. The pretreatment of rats with IL-8 (1 μg/300g) decreased the number of PMNs migrating to abdominal cavity, and tended to decrease their ability to synthetise NO. The suggested by Gimbrone et al. (1989) anti-inflammatory properties of a circulating form of IL-8 seems to be related ...

查看原文本刊更多论文

白细胞介素-8对选择素P (PADGEM GMP-140)介导的人血小板体外粘附中性粒细胞和免疫复合物诱导的大鼠腹膜炎的影响

根据Jungi(1986)的研究，通过人凝胶过滤凝血酶(30-50 mU)刺激的血小板在分离的同源中性粒细胞(PMNs)表面形成“玫瑰花”来测量选择素- p的表达。单克隆抗选择素p抗体完全阻止了“玫瑰花”的形成，证明了选择素- p介导粘附的特异性。IL-8 (50-200 ng/ml)浓度依赖性地抑制血小板对PMNs的粘附。阿司匹林和L-NO2Arg均未改变IL-8的抑制活性。腹腔注射ova - antiyova复合物2小时后，大鼠腹膜炎的发生与腹腔内pmn和蛋白的积累有关。IL-8 (1 μg/300g)预处理大鼠可减少PMNs向腹腔迁移的数量，并有降低其合成NO能力的趋势。Gimbrone等人(1989)认为循环形式的IL-8的抗炎特性似乎与…

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Endothelium-journal of Endothelial Cell Research

自引率

0.00%

发文量